LOCUS       sequence-230412-        9193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express sgZFP64e2 resistant cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9193)
  AUTHORS   Lu B, Klingbeil O, Tarumoto Y, Somerville TDD, Huang YH, Wei Y, Wai 
            DC, Low JKK, Milazzo JP, Wu XS, Cao Z, Yan X, Demerdash OE, Huang G,
            Mackay JP, Kinney JB, Shi J, Vakoc CR
  TITLE     A Transcription Factor Addiction in Leukemia Imposed by the MLL 
            Promoter Sequence.
  JOURNAL   Cancer Cell. 2018 Dec 10;34(6):970-981.e8. doi: 
            10.1016/j.ccell.2018.10.015. Epub 2018 Nov 29.
  PUBMED    30503706
REFERENCE   2  (bases 1 to 9193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.10.015"; journalName: "Cancer Cell"; date: 
            "2018-12-10- 10"; volume: "34"; issue: "6"; pages: "970-981.e8"
COMMENT     Sequence Label: Lenti_Neo_WT_ZFP64_CR
FEATURES             Location/Qualifiers
     source          1..9193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..212
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2290..2346
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2365..3159
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2419..2438)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3029..3048
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    3175..3763
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3228..3248)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3646..3657)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3835..4068
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4146..4267
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4183..4202)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4237..4256
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4429..4448)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4430..4448)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4458..4475)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4458..4474)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4467..4489)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4616..5071
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4703..4722)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4913..4934
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5097..5201
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5202..5270,5271..6062)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5420..5439)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6233..6821
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6722..6741
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6975..6992
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7109..7130
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7145..7150,7151..7168,7169..7175)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7183..7199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7188..7210
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7207..7223
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7207..7223
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7242..7262
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7244..7262
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7290..7516
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7517..7697
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7744..7869
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8362..8595
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8780..8824
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9008..9125
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
       61 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
      121 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
      181 tttttcgcaa cgggtttgcc gccagaacac aggtgtcgtg acgcgggatc cgccaccatg
      241 aacgcgagca gcgagggcga gagcttcgcg ggctcggtgc aaattccagg tggcacaacg
      301 gtgctggtgg agctgactcc cgacatccac atttgtggaa tttgtaaaca gcagtttaac
      361 aacctggatg cctttgtagc tcacaagcaa agtggctgcc agctgacagg cacatccgca
      421 gcagccccca gcacggtcca gtttgtatcg gaggaaacag tgcctgccac ccagactcag
      481 accaccacca gaaccatcac ctcggagacc cagacaatca cagtttcagc tccagaattt
      541 gtttttgaac atggctatca aacttacctg cccacggaaa gtaatgaaaa ccagacagcc
      601 actgtcatct ctctccctgc caagtcacgc accaaaaagc ccacaacacc acctgctcag
      661 aaaaggctta actgttgcta tccaggttgc caattcaaga ctgcttatgg catgaaggac
      721 atggagcggc atttaaaaat tcacacggga gacaaacccc ataagtgtga agtctgtggc
      781 aagtgcttta gccggaaaga caagctgaaa actcacatgc ggtgccacac gggcgtgaag
      841 ccctacaagt gtaagacgtg tgactacgcc gctgccgaca gcagcagcct caacaagcac
      901 ctgaggatcc actcggacga gcggcccttc aaatgccaga tctgccccta cgccagccgc
      961 aactccagcc agctcactgt ccacctgcga tcccacacgg gggacgcccc cttccagtgc
     1021 tggctctgta gcgccaagtt caaaatcagc tcggacttga aaaggcacat gcgggtgcac
     1081 tcgggggaga agcctttcaa gtgcgagttc tgcaatgtcc gctgcaccat gaaggggaac
     1141 ctcaagtcgc acatccgtat caagcacagc gggaataact tcaagtgtcc tcattgcgac
     1201 ttcctgggtg acagcaaagc caccctccgg aagcacagcc gcgtgcacca gtcggagcat
     1261 cctgagaagt gctcggaatg cagctactcc tgctccagca aggccgccct gcgcatccac
     1321 gagcgtatcc actgcaccga ccgccctttc aagtgcaact actgcagctt cgacaccaaa
     1381 cagcccagca acctgagcaa gcacatgaag aagttccacg gggacatggt taagactgag
     1441 gctctagaga ggaaggacac cggcaggcag agcagccggc aggtggccaa gctggatgcc
     1501 aagaagagtt tccactgcga tatatgcgat gcctccttca tgcgggagga ctcgctccgc
     1561 agccacaaga gacagcacag tgagtacagt gagagtaaga actcggacgt gaccgttctc
     1621 cagtttcaga tcgaccccag caagcagccc gccacgcccc tcactgtggg acacctccag
     1681 gtgcccctcc agcccagcca agtgccccag ttcagcgagg gaagagtcaa aatcatcgtt
     1741 gggcatcagg tgccccaggc gaacaccatc gtccaggctg ccgctgctgc agtgaacatc
     1801 gtcccgcctg ccttggtggc ccagaaccca gaggaactcc cagggaacag ccggctgcag
     1861 atcctgcgcc aggtcagtct gatcgccccc cctcagtcct cgcggtgtcc gagcgaggcg
     1921 ggcgcaatga cccagccggc tgtcctgctg accacccacg agcagacgga cggagccact
     1981 ctgcaccaga ctctcatccc cacggcctca ggtggccccc aggaaggctc tggcaatcaa
     2041 actttcatta ccagttcggg tattacttgc actgactttg aaggcctaaa cgccttgatt
     2101 caggagggga cagcagaagt gacagtggtg agcgatggag gccagaacat cgcagtggcc
     2161 accacagcgc caccggtctt ctcctcctct tcccagcaag aactacccaa gcagacctac
     2221 tccatcattc aaggggcagc ccatccagct ttgctctgtc ccgccgactc cattccagat
     2281 ggcagcggcg ccaccaactt cagcctgctg aagcaggccg gcgacgtgga ggagaacccc
     2341 ggccccatga ccgagtacaa gcccatgatt gaacaagatg gattgcacgc aggttctccg
     2401 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     2461 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     2521 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     2581 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     2641 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     2701 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     2761 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     2821 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     2881 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     2941 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     3001 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     3061 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     3121 cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa cgcgttaagt cgacaatcaa
     3181 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3241 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3301 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     3361 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     3421 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     3481 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3541 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     3601 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     3661 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     3721 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     3781 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     3841 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     3901 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     3961 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     4021 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     4081 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     4141 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     4201 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     4261 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgccccatgg
     4321 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     4381 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     4441 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     4501 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     4561 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     4621 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4681 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4741 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4801 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4861 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4921 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     4981 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5041 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     5101 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5161 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5221 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5281 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5341 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5401 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     5461 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5521 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5581 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     5641 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     5701 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     5761 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     5821 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     5881 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     5941 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6001 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     6061 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6121 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6181 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6241 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6301 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6361 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6421 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6481 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6541 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6601 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6661 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6721 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6781 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6841 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6901 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     6961 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     7021 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     7081 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     7141 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     7201 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     7261 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     7321 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
     7381 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
     7441 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
     7501 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7561 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7621 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7681 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     7741 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     7801 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     7861 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     7921 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     7981 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     8041 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     8101 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     8161 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     8221 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     8281 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     8341 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     8401 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     8461 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     8521 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     8581 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     8641 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     8701 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     8761 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     8821 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     8881 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     8941 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccag
     9001 accacaattt taaaagaaaa ggggggattg ggaggtacag tgcaggggaa agaatagtag
     9061 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     9121 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gagtggctcc
     9181 ggtgcccgtc agt
//
