LOCUS       sequence-230418-        1266 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral transduction and expression of a CRISPR/Cas9-resistant 
            PDHA2 into any mammalian cell (when using sgRNA 
            5'-ATGTAATGACGTGATCCGAG-3').
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Moutaoufik MT, Malty R, Amin S, Zhang Q, Phanse S, Gagarinova A, 
            Zilocchi M, Hoell L, Minic Z, Gagarinova M, Aoki H, Stockwell J, 
            Jessulat M, Goebels F, Broderick K, Scott NE, Vlasblom J, Musso G, 
            Prasad B, Lamantea E, Garavaglia B, Rajput A, Murayama K, Okazaki Y,
            Foster LJ, Bader GD, Cayabyab FS, Babu M
  TITLE     Rewiring of the Human Mitochondrial Interactome during Neuronal 
            Reprogramming Reveals Regulators of the Respirasome and 
            Neurogenesis.
  JOURNAL   iScience. 2019 Sep 4;19:1114-1132. doi: 10.1016/j.isci.2019.08.057.
  PUBMED    31536960
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2019-09-4"; volume: "19:1114-1132. doi"; pages: " 
            10.1016/j.isci.2019.08.057"
COMMENT     Sequence Label: pLEX307_CR-PDHA2
FEATURES             Location/Qualifiers
     source          1..1266
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             252..263
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 crraaatttc tctttmmgtg tcgtgaggct agcatgctgg ccgccttcat ctcccgcgtg
       61 ttgaggcgag ttgcccagaa atcagctcgc agagtgctgg tggcatcccg taactcctca
      121 aatgacgcta catttgaaat taagaaatgt gatctttatc tgttggaaga gggtccccct
      181 gtcactacag tgctcactag ggcggagggg cttaaatact acaggatgat gctgactgtt
      241 cgccgcatgg aattgaaggc agatcagctg tacaaacaga aattcattcg cggtttctgt
      301 cacctgtgcg atggtcagga agcttgttgc gtgggccttg aggccggcat aaatccctcg
      361 gatcacgtca ttacatccta tagggctcat ggtgtgtgct atactcgggg actttctgtc
      421 cgatccattc tcgcagagct gacgggaaga agaggaggtt gtgctaaagg aaaaggagga
      481 tcgatgcata tgtataccaa gaacttctat gggggcaatg gcatcgtcgg tgcacagggc
      541 cccctgggcg ctggcattgc tctggcctgt aaatataaag gaaacgatga gatctgtttg
      601 actttatatg gggatggcgc tgcgaatcag gggcagatag ccgaagcttt caatatggca
      661 gctttatgga aattaccttg tgttttcatc tgtgagaata acctatatgg aatgggaaca
      721 tctactgaga gagcagcagc cagccctgat tactacaaga ggggcaattt tatccctggg
      781 ctaaaggtcg atggaatgga tgttctgtgt gttcgtgagg caacaaaatt tgcagctaac
      841 tactgtagat ctggaaaggg gcccatactg atggagctgc aaacctaccg ttatcatgga
      901 cacagtatga gtgatcctgg agtcagttat cgtacacgag aagaaattca ggaagtaaga
      961 agtaagaggg atcctataat aattctccaa gatagaatgg taaacagcaa gctcgccact
     1021 gtgaagaatt aaggaaattg gggctgagtg agaagaattg atgatgctgc cagttgctac
     1081 mctgatcctg agcmcattgg agattaggcc atcacatcta cgcagtgatc atctttgagt
     1141 cgtggtgcaa tcatgatcag ttagtcgtca gtactagtac cgcgtagtcg acatcaccty
     1201 gatamaattg agatgactgg aatyctacwg tgctctttac gcarggatac gccgygcctt
     1261 aakgcc
//
