LOCUS       sequence-230425-        1273 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral transduction and expression of CRISPR/Cas9-resistant 
            PDHA2S291D/S293D into any mammalian cell (when using sgRNA 
            5'-ATGTAATGACGTGATCCGAG-3').
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Jessulat M, Amin S, Hooshyar M, Malty R, Moutaoufik MT, Zilocchi M, 
            Istace Z, Phanse S, Aoki H, Omidi K, Burnside D, Samanfar B, Aly KA,
            Golshani A, Babu M
  TITLE     The conserved Tpk1 regulates non-homologous end joining 
            double-strand break repair by phosphorylation of Nej1, a homolog of 
            the human XLF.
  JOURNAL   Nucleic Acids Res. 2021 Jul 9. pii: 6318499. doi: 
            10.1093/nar/gkab585.
  PUBMED    34244791
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Jul 9. pii: 6318499. doi: 10.1093/nar/gkab585."
COMMENT     Sequence Label: pLEX307_CR-PDHA2S291D/S293D
FEATURES             Location/Qualifiers
     source          1..1273
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             252..263
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 araastttct tatttmtgtg tcgtgaggct agcatgctgg ccgccttcat ctcccgcgtg
       61 ttgaggcgag ttgcccagaa atcagctcgc agagtgctgg tggcatcccg taactcctca
      121 aatgacgcta catttgaaat taagaaatgt gatctttatc tgttggaaga gggtccccct
      181 gtcactacag tgctcactag ggcggagggg cttaaatact acaggatgat gctgactgtt
      241 cgccgcatgg aattgaaggc agatcagctg tacaaacaga aattcattcg cggtttctgt
      301 cacctgtgcg atggtcagga agcttgttgc gtgggccttg aggccggcat aaatccctcg
      361 gatcacgtca ttacatccta tagggctcat ggtgtgtgct atactcgggg actttctgtc
      421 cgatccattc tcgcagagct gacgggaaga agaggaggtt gtgctaaagg aaaaggagga
      481 tcgatgcata tgtataccaa gaacttctat gggggcaatg gcatcgtcgg tgcacagggc
      541 cccctgggcg ctggcattgc tctggcctgt aaatataaag gaaacgatga gatctgtttg
      601 actttatatg gggatggcgc tgcgaatcag gggcagatag ccgaagcttt caatatggca
      661 gctttatgga aattaccttg tgttttcatc tgtgagaata acctatatgg aatgggaaca
      721 tctactgaga gagcagcagc cagccctgat tactacaaga ggggcaattt tatccctggg
      781 ctaaaggtcg atggaatgga tgttctgtgt gttcgtgagg caacaaaatt tgcagctaac
      841 tactgtagat ctggaaaggg gcccatactg atggagctgc aaacctaccg ttatcatgga
      901 cacgacatgg acgatcctgg agtcagttat cgtacacgag aagaaattca cgaagtaaga
      961 agtaagaggg atcctataat aattctccaa gatagaatgg taaacagcaa gctcgccact
     1021 gtgaagaatt aaaggaaatt ggggctgagt gaggaaagaa attgatgatg ctgccagttt
     1081 gctaccactg atcctgagcm cattggagat aggcatcaca tctacagcag tgatcatctt
     1141 tgagtcgtgt gcaatccatg atcagttagt cgtcagtaac tagtacgcgt agtcgacatc
     1201 acctctgata caattgggag atgactgatt ctaccwgtgc tctttascag tggaacgcck
     1261 ccttattgcc tkg
//
