LOCUS       sequence-230427-        1271 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral transduction and expression of CRISPR/Cas9-resistant 
            PDHA2S291A into any mammalian cell (when using sgRNA 
            5'-ATGTAATGACGTGATCCGAG-3').
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Moutaoufik MT, Malty R, Amin S, Zhang Q, Phanse S, Gagarinova A, 
            Zilocchi M, Hoell L, Minic Z, Gagarinova M, Aoki H, Stockwell J, 
            Jessulat M, Goebels F, Broderick K, Scott NE, Vlasblom J, Musso G, 
            Prasad B, Lamantea E, Garavaglia B, Rajput A, Murayama K, Okazaki Y,
            Foster LJ, Bader GD, Cayabyab FS, Babu M
  TITLE     Rewiring of the Human Mitochondrial Interactome during Neuronal 
            Reprogramming Reveals Regulators of the Respirasome and 
            Neurogenesis.
  JOURNAL   iScience. 2019 Sep 4;19:1114-1132. doi: 10.1016/j.isci.2019.08.057.
  PUBMED    31536960
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2019-09-4"; volume: "19:1114-1132. doi"; pages: " 
            10.1016/j.isci.2019.08.057"
COMMENT     Sequence Label: pLEX307_CR-PDHA2S291A
FEATURES             Location/Qualifiers
     source          1..1271
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             251..262
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aracttttct atttmtgtgt cgtgaggcta gcatgctggc cgccttcatc tcccgcgtgt
       61 tgaggcgagt tgcccagaaa tcagctcgca gagtgctggt ggcatcccgt aactcctcaa
      121 atgacgctac atttgaaatt aagaaatgtg atctttatct gttggaagag ggtccccctg
      181 tcactacagt gctcactagg gcggaggggc ttaaatacta caggatgatg ctgactgttc
      241 gccgcatgga attgaaggca gatcagctgt acaaacagaa attcattcgc ggtttctgtc
      301 acctgtgcga tggtcaggaa gcttgttgcg tgggccttga ggccggcata aatccctcgg
      361 atcacgtcat tacatcctat agggctcatg gtgtgtgcta tactcgggga ctttctgtcc
      421 gatccattct cgcagagctg acgggaagaa gaggaggttg tgctaaagga aaaggaggat
      481 cgatgcatat gtataccaag aacttctatg ggggcaatgg catcgtcggt gcacagggcc
      541 ccctgggcgc tggcattgct ctggcctgta aatataaagg aaacgatgag atctgtttga
      601 ctttatatgg ggatggcgct gcgaatcagg ggcagatagc cgaagctttc aatatggcag
      661 ctttatggaa attaccttgt gttttcatct gtgagaataa cctatatgga atgggaacat
      721 ctactgagag agcagcagcc agccctgatt actacaagag gggcaatttt atccctgggc
      781 taaaggtcga tggaatggat gttctgtgtg ttcgtgaggc aacaaaattt gcagctaact
      841 actgtagatc tggaaagggg cccatactga tggagctgca aacctaccgt tatcatggac
      901 acgccatgag tgatcctgga gtcagttatc gtacacgaga agaaattcag gagtaagaag
      961 taagagggat cctataataa ttctccaaga tagaatggta aacagcaagc tcgccactgt
     1021 ggaagaatta aaggaaattg gggctgagtg agaagaatga tgatgctgcc agttgctacc
     1081 actgatctga gcacatttga gatagcatca catctacagc atgatcatct tttgagtcgt
     1141 ggtgcaatcc atgatcagtt agtcgtcagt aaactagtac scgtagtcga catcacctct
     1201 gatmaatgga gaatgactgg aatctamcaa tgtgtccttt acgctatgtg gaacgccygc
     1261 tttatagkgc c
//
