LOCUS       sequence-230428-        1258 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral transduction and expression of CRISPR/Cas9-resistant 
            PDHA2S293A into any mammalian cell (when using sgRNA 
            5'-ATGTAATGACGTGATCCGAG-3').
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Moutaoufik MT, Malty R, Amin S, Zhang Q, Phanse S, Gagarinova A, 
            Zilocchi M, Hoell L, Minic Z, Gagarinova M, Aoki H, Stockwell J, 
            Jessulat M, Goebels F, Broderick K, Scott NE, Vlasblom J, Musso G, 
            Prasad B, Lamantea E, Garavaglia B, Rajput A, Murayama K, Okazaki Y,
            Foster LJ, Bader GD, Cayabyab FS, Babu M
  TITLE     Rewiring of the Human Mitochondrial Interactome during Neuronal 
            Reprogramming Reveals Regulators of the Respirasome and 
            Neurogenesis.
  JOURNAL   iScience. 2019 Sep 4;19:1114-1132. doi: 10.1016/j.isci.2019.08.057.
  PUBMED    31536960
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2019-09-4"; volume: "19:1114-1132. doi"; pages: " 
            10.1016/j.isci.2019.08.057"
COMMENT     Sequence Label: pLEX307_CR-PDHA2S293A
FEATURES             Location/Qualifiers
     source          1..1258
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             250..261
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agactttcta tttmtgtgtc gtgaggctag catgctggcc gccttcatct cccgcgtgtt
       61 gaggcgagtt gcccagaaat cagctcgcag agtgctggtg gcatcccgta actcctcaaa
      121 tgacgctaca tttgaaatta agaaatgtga tctttatctg ttggaagagg gtccccctgt
      181 cactacagtg ctcactaggg cggaggggct taaatactac aggatgatgc tgactgttcg
      241 ccgcatggaa ttgaaggcag atcagctgta caaacagaaa ttcattcgcg gtttctgtca
      301 cctgtgcgat ggtcaggaag cttgttgcgt gggccttgag gccggcataa atccctcgga
      361 tcacgtcatt acatcctata gggctcatgg tgtgtgctat actcggggac tttctgtccg
      421 atccattctc gcagagctga cgggaagaag aggaggttgt gctaaaggaa aaggaggatc
      481 gatgcatatg tataccaaga acttctatgg gggcaatggc atcgtcggtg cacagggccc
      541 cctgggcgct ggcattgctc tggcctgtaa atataaagga aacgatgaga tctgtttgac
      601 tttatatggg gatggcgctg cgaatcaggg gcagatagcc gaagctttca atatggcagc
      661 tttatggaaa ttaccttgtg ttttcatctg tgagaataac ctatatggaa tgggaacatc
      721 tactgagaga gcagcagcca gccctgatta ctacaagagg ggcaatttta tccctgggct
      781 aaaggtcgat ggaatggatg ttctgtgtgt tcgtgaggca acaaaatttg cagctaacta
      841 ctgtagatct ggaaaggggc ccatactgat ggagctgcaa acctaccgtt atcatggaca
      901 cagtatggcc gatcctggag tcagttatcg tacacgagaa gaaattcaga agtaagaagt
      961 aagagggatc ctataataat tctccaagat agaatggtaa acagcaagct cgccactgtg
     1021 gaagattaaa ggaaattggg gctgagtgag aagaatgatg atgctgccag tttgctacac
     1081 tgatctgagc acatttgaga tagcatcaca tctacagcag tgatcatctt tgagtcgkgt
     1141 gcaatcatga tcagtagtcg tcagtaacta gtaccgtaag tcgamatcaa ctctgatmaa
     1201 tgagatgact gatctaacwg tgctctacgc wwtgtggata cgcygcctta atgccgta
//
