LOCUS       sequence-230431-        1258 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral transduction and expression of CRISPR/Cas9-resistant 
            PDHA2S291A/S293A into any mammalian cell (when using sgRNA 
            5'-ATGTAATGACGTGATCCGAG-3').
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Moutaoufik MT, Malty R, Amin S, Zhang Q, Phanse S, Gagarinova A, 
            Zilocchi M, Hoell L, Minic Z, Gagarinova M, Aoki H, Stockwell J, 
            Jessulat M, Goebels F, Broderick K, Scott NE, Vlasblom J, Musso G, 
            Prasad B, Lamantea E, Garavaglia B, Rajput A, Murayama K, Okazaki Y,
            Foster LJ, Bader GD, Cayabyab FS, Babu M
  TITLE     Rewiring of the Human Mitochondrial Interactome during Neuronal 
            Reprogramming Reveals Regulators of the Respirasome and 
            Neurogenesis.
  JOURNAL   iScience. 2019 Sep 4;19:1114-1132. doi: 10.1016/j.isci.2019.08.057.
  PUBMED    31536960
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2019-09-4"; volume: "19:1114-1132. doi"; pages: " 
            10.1016/j.isci.2019.08.057"
COMMENT     Sequence Label: pLEX307_CR-PDHA2S291A/S293A
FEATURES             Location/Qualifiers
     source          1..1258
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             251..262
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gggactttct atttmggtgt cgtgaggcta gcatgctggc cgccttcatc tcccgcgtgt
       61 tgaggcgagt tgcccagaaa tcagctcgca gagtgctggt ggcatcccgt aactcctcaa
      121 atgacgctac atttgaaatt aagaaatgtg atctttatct gttggaagag ggtccccctg
      181 tcactacagt gctcactagg gcggaggggc ttaaatacta caggatgatg ctgactgttc
      241 gccgcatgga attgaaggca gatcagctgt acaaacagaa attcattcgc ggtttctgtc
      301 acctgtgcga tggtcaggaa gcttgttgcg tgggccttga ggccggcata aatccctcgg
      361 atcacgtcat tacatcctat agggctcatg gtgtgtgcta tactcgggga ctttctgtcc
      421 gatccattct cgcagagctg acgggaagaa gaggaggttg tgctaaagga aaaggaggat
      481 cgatgcatat gtataccaag aacttctatg ggggcaatgg catcgtcggt gcacagggcc
      541 ccctgggcgc tggcattgct ctggcctgta aatataaagg aaacgatgag atctgtttga
      601 ctttatatgg ggatggcgct gcgaatcagg ggcagatagc cgaagctttc aatatggcag
      661 ctttatggaa attaccttgt gttttcatct gtgagaataa cctatatgga atgggaacat
      721 ctactgagag agcagcagcc agccctgatt actacaagag gggcaatttt atccctgggc
      781 taaaggtcga tggaatggat gttctgtgtg ttcgtgaggc aacaaaattt gcagctaact
      841 actgtagatc tggaaagggg cccatactga tggagctgca aacctaccgt tatcatggac
      901 acgccatggc cgatcctgga gtcagttatc gtacacgaga agaaattcag gaagtaagaa
      961 gtaagaggga tcctataata attctccaag atagaatggt aaacagcaag ctcgcactgt
     1021 gagattaaag gaaattgggg ctgagtgaga agaattgatg atgctgccag tttgctacac
     1081 tgatctgagc acatttgaga tagcatcmat ctacagcagt gatcatcttt gagtcgtggt
     1141 gcaatcatga tcagtagtcg tcagtaaact agtacgcgtt agtcgacatc aacctctgga
     1201 tacaattggg agatgactgg tatctacwgt gcyctttacs cwgkgaatcc cgcttatc
//
