LOCUS       sequence-23044-e         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Zawel L, Yu J, Torrance CJ, Markowitz S, Kinzler KW, Vogelstein B, 
            Zhou S
  TITLE     DEC1 is a downstream target of TGF-beta with sequence-specific 
            transcriptional repressor activities.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Mar 5. 99(5):2848-53.
  PUBMED    11880636
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2002 Mar 5. 99(5):2848-53."
COMMENT     Sequence Label: SMAD4 KO Neo
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     132..154
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     polyA_signal    297..431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(334..353)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     388..407
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(449..475)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YAYDVPDYA"
ORIGIN
        1 ggcggccgct ctagcgggcc ccccctcgat cgacctcatg gctgcgcccc gacacccgcc
       61 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
      121 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
      181 gaggcagccg gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
      241 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
      301 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
      361 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
      421 atgtctggat cggccgctat ccttagctag cgtaatctgg aacatcgtaa gcgtaatctg
      481 gaacatcgta ctctttatcg cccagcagaa cggattccag tgcgattttg atcatgtcgt
      541 tgaaggtggt ctgacgctca gcggcagtgg tctgctcgtg agtgcggatg tggtcagaca
      601 cggtgcagat ggtcagggct ttcacgccaa actctgcagc gacgccgtag ataccagccg
      661 cttccatttc cacgccgagg atgccgtatt ttt
//
