LOCUS       sequence-230441-       16382 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The F40-based human desmoplakin I tension sensor detects forces in 
            the range of 1-6 pN by changes in FRET between YPet(short) and 
            mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16382)
  AUTHORS   Price AJ, Cost AL, Ungewiss H, Waschke J, Dunn AR, Grashoff C
  TITLE     Mechanical loading of desmosomes depends on the magnitude and 
            orientation of external stress.
  JOURNAL   Nat Commun. 2018 Dec 11;9(1):5284. doi: 10.1038/s41467-018-07523-0.
  PUBMED    30538252
REFERENCE   2  (bases 1 to 16382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-11"; volume: "9(1):5284. doi"; pages: " 
            10.1038/s41467-018-07523-0"
COMMENT     Sequence Label: Desmoplakin I tension sensor (F40-based)
FEATURES             Location/Qualifiers
     source          1..16382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             414..589
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    652..851
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1052..1468
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1398..1420
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1436..1452
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1565..2164
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1565..1584)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2061..2081
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     enhancer        2339..2642
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2643..2846
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2797..2817
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(9089..9108)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             9599..10306
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9744..9762)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     10012..10031
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             13121..13714
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(13817..13839)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    13967..14000
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    14060..14093
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     14113..14132
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(14213..14230)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14384..14972)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14464..14483)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15143..15934,15935..16003))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15766..15785
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16004..16108)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     16176..16194
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
     1441 tccagccctc actccttctc taggcgccgg aatgatcccc cgggctgcag gtcggccgcc
     1501 acgaccggtg ccgccaccat cccctgaccc acgcccctga cccctcacaa ggagacgacc
     1561 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     1621 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     1681 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     1741 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     1801 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     1861 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     1921 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     1981 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     2041 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     2101 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     2161 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatggctc
     2221 cgaccgaagc cacccggggc ggccccgccg accccgcacc cgcccccgag gcccaccgac
     2281 tctagtaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     2341 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     2401 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     2461 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     2521 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     2581 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     2641 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     2701 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     2761 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     2821 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
     2881 ggactcagat ctgggcccat aaagcttata cgaattcacc atgagctgca acggaggctc
     2941 ccacccgcgg atcaacactc tgggccgcat gatccgcgcc gagtctggcc cggacctgcg
     3001 ctacgaggtg accagcggcg gcgggggcac cagcaggatg tactattctc ggcgcggcgt
     3061 gatcaccgac cagaactcgg acggctactg tcaaaccggc acgatgtcca ggcaccagaa
     3121 ccagaacacc atccaggagc tgctgcagaa ctgctccgac tgcttgatgc gagcagagct
     3181 catcgtgcag cctgaattga agtatggaga tggaatacaa ctgactcgga gtcgagaatt
     3241 ggatgagtgt tttgcccagg ccaatgacca aatggaaatc ctcgacagct tgatcagaga
     3301 gatgcggcag atgggccagc cctgtgatgc ttaccagaaa aggcttcttc agctccaaga
     3361 gcaaatgcga gccctttata aagccatcag tgtcccacga gtccgcaggg ccagctccaa
     3421 gggtggtgga ggctacactt gtcagagtgg ctctggctgg gatgagttca ccaaacatgt
     3481 caccagtgaa tgtttggggt ggatgaggca gcaaagggcg gagatggaca tggtggcctg
     3541 gggtgtggac ctggcctcag tggagcagca cattaacagc caccggggca tccacaactc
     3601 catcggcgac tatcgctggc agctggacaa aatcaaagcc gacctgcgcg agaaatctgc
     3661 gatctaccag ttggaggagg agtatgaaaa cctgctgaaa gcgtcctttg agaggatgga
     3721 tcacctgcga cagctgcaga acatcattca ggccacgtcc agggagatca tgtggatcaa
     3781 tgactgcgag gaggaggagc tgctgtacga ctggagcgac aagaacacca acatcgctca
     3841 gaaacaggag gccttctcca tacgcatgag tcaactggaa gttaaagaaa aagagctcaa
     3901 taagctgaaa caagaaagtg accaacttgt cctcaatcag catccagctt cagacaaaat
     3961 tgaggcctat atggacactc tgcagacgca gtggagttgg attcttcaga tcaccaagtg
     4021 cattgatgtt catctgaaag aaaatgctgc ctactttcag ttttttgaag aggcgcagtc
     4081 tactgaagca tacctgaagg ggctccagga ctccatcagg aagaagtacc cctgcgacaa
     4141 gaacatgccc ctgcagcacc tgctggaaca gatcaaggag ctggagaaag aacgagagaa
     4201 aatccttgaa tacaagcgtc aggtgcagaa cttggtaaac aagtctaaga agattgtaca
     4261 gctgaagcct cgtaacccag actacagaag caataaaccc attattctca gagctctctg
     4321 tgactacaaa caagatcaga aaatcgtgca taagggggat gagtgtatcc tgaaggacaa
     4381 caacgagcgc agcaagtggt acgtgacggg cccgggaggc gttgacatgc ttgttccctc
     4441 tgtggggctg atcatccctc ctccgaaccc actggccgtg gacctctctt gcaagattga
     4501 gcagtactac gaagccatct tggctctgtg gaaccagctc tacatcaaca tgaagagcct
     4561 ggtgtcctgg cactactgca tgattgacat agagaagatc agggccatga caatcgccaa
     4621 gctgaaaaca atgcggcagg aagattacat gaagacgata gccgaccttg agttacatta
     4681 ccaagagttc atcagaaata gccaaggctc agagatgttt ggagatgatg acaagcggaa
     4741 aatacagtct cagttcaccg atgcccagaa gcattaccag accctggtca ttcagctccc
     4801 tggctatccc cagcaccaga cagtgaccac aactgaaatc actcatcatg gaacctgcca
     4861 agatgtcaac cataataaag taattgaaac caacagagaa aatgacaagc aagaaacatg
     4921 gatgctgatg gagctgcaga agattcgcag gcagatagag cactgcgagg gcaggatgac
     4981 tctcaaaaac ctccctctag cagaccaggg gtcttctcac cacatcacag tgaaaattaa
     5041 cgagcttaag agtgtgcaga atgattcaca agcaattgct gaggttctca accagcttaa
     5101 agatatgctt gccaacttca gaggttctga aaagtactgc tatttacaga atgaagtatt
     5161 tggactattt cagaaactgg aaaatatcaa tggtgttaca gatggctact taaatagctt
     5221 atgcacagta agggcactgc tccaggctat tctccaaaca gaagacatgt taaaggttta
     5281 tgaagccagg ctcactgagg aggaaactgt ctgcctggac ctggataaag tggaagctta
     5341 ccgctgtgga ctgaagaaaa taaaaaatga cttgaacttg aagaagtcgt tgttggccac
     5401 tatgaagaca gaactacaga aagcccagca gatccactct cagacttcac agcagtatcc
     5461 actttatgat ctggacttgg gcaagttcgg tgaaaaagtc acacagctga cagaccgctg
     5521 gcaaaggata gataaacaga tcgactttag attatgggac ctggagaaac aaatcaagca
     5581 attgaggaat tatcgtgata actatcaggc tttctgcaag tggctctatg atgctaaacg
     5641 ccgccaggat tccttagaat ccatgaaatt tggagattcc aacacagtca tgcggttttt
     5701 gaatgagcag aagaacttgc acagtgaaat atctggcaaa cgagacaaat cagaggaagt
     5761 acaaaaaatt gctgaacttt gcgccaattc aattaaggat tatgagctcc agctggcctc
     5821 atacacctca ggactggaaa ctctgctgaa catacctatc aagaggacca tgattcagtc
     5881 cccttctggg gtgattctgc aagaggctgc agatgttcat gctcggtaca ttgaactact
     5941 tacaagatct ggagactatt acaggttctt aagtgagatg ctgaagagtt tggaagatct
     6001 gaagctgaaa aataccaaga tcgaagtttt ggaagaggag ctcagactgg cccgagatgc
     6061 caactcggaa aactgtaata agaacaaatt cctggatcag aacctgcaga aataccaggc
     6121 agagtgttcc cagttcaaag cgaagcttgc gagcctggag gagctgaaga gacaggctga
     6181 gctggatggg aagtcggcta agcaaaatct agacaagtgc tacggccaaa taaaagaact
     6241 caatgagaag atcacccgac tgacttatga gattgaagat gaaaagagaa gaagaaaatc
     6301 tgtggaagac agatttgacc aacagaagaa tgactatgac caactgcaga aagcaaggca
     6361 atgtgaaaag gagaaccttg gttggcagaa attagagtct gagaaagcca tcaaggagaa
     6421 ggagtacgag attgaaaggt tgagggttct actgcaggaa gaaggcaccc ggaagagaga
     6481 atatgaaaat gagctggcaa aggtaagaaa ccactataat gaggagatga gtaatttaag
     6541 gaacaagtat gaaacagaga ttaacattac gaagaccacc atcaaggaga tatccatgca
     6601 aaaagaggat gattccaaaa atcttagaaa ccagcttgat agactttcaa gggaaaatcg
     6661 agatctgaag gatgaaattg tcaggctcaa tgacagcatc ttgcaggcca ctgagcagcg
     6721 aaggcgagct gaagaaaacg cccttcagca aaaggcctgt ggctctgaga taatgcagaa
     6781 gaagcagcat ctggagatag aactgaagca ggtcatgcag cagcgctctg aggacaatgc
     6841 ccggcacaag cagtccctgg aggaggctgc caagaccatt caggacaaaa ataaggagat
     6901 cgagagactc aaagctgagt ttcaggagga ggccaagcgc cgctgggaat atgaaaatga
     6961 actgagtaag gtaagaaaca attatgatga ggagatcatt agcttaaaaa atcagtttga
     7021 gaccgagatc aacatcacca agaccaccat ccaccagctc accatgcaga aggaagagga
     7081 taccagtggc taccgggctc agatagacaa tctcacccga gaaaacagga gcttatctga
     7141 agaaataaag aggctgaaga acactctaac ccagaccaca gagaatctca ggagggtgga
     7201 agaagacatc caacagcaaa aggccactgg ctctgaggtg tctcagagga aacagcagct
     7261 ggaggttgag ctgagacaag tcactcagat gcgaacagag gagagcgtaa gatataagca
     7321 atctcttgat gatgctgcca aaaccatcca ggataaaaac aaggagatag aaaggttaaa
     7381 acaactgatc gacaaagaaa caaatgaccg gaaatgcctg gaagatgaaa acgcgagatt
     7441 acaaagggtc cagtatgacc tgcagaaagc aaacagtagt gcgacggaga caataaacaa
     7501 actgaaggtt caggagcaag aactgacacg cctgaggatc gactatgaaa gggtttccca
     7561 ggagaggact gtgaaggacc aggatatcac gcggttccag aactctctga aagagctgca
     7621 gctgcagaag cagaaggtgg aagaggagct gaatcggctg aagaggaccg cgtcagaaga
     7681 ctcctgcaag aggaagaagc tcgaggaaga gctggaaggc atgaggaggt cgctgaagga
     7741 gcaagccatc aaaatcacca acctgaccca gcagctggag caggcatcca ttgttaagaa
     7801 gaggagtgag gatgacctcc ggcagcagag ggacgtgctg gatggccacc tgagggaaaa
     7861 gcagaggacc caggaagagc tgaggaggct ctcttctgag gtcgaggccc tgaggcggca
     7921 gttactccag gaacaggaaa gtgtcaaaca agctcacttg aggaatgagc atttccagaa
     7981 ggcgatagaa gataaaagca gaagcttaaa tgaaagcaaa atagaaattg agaggctgca
     8041 gtctctcaca gagaacctga ccaaggagca cttgatgtta gaagaagaac tgcggaacct
     8101 gaggctggag tacgatgacc tgaggagagg acgaagcgaa gcggacagtg ataaaaatgc
     8161 aaccatcttg gaactaagga gccagctgca gatcagcaac aaccggaccc tggaactgca
     8221 ggggctgatt aatgatttac agagagagag ggaaaatttg agacaggaaa ttgagaaatt
     8281 ccaaaagcag gctttagagg catctaatag gattcaggaa tcaaagaatc agtgtactca
     8341 ggtggtacag gaaagagaga gccttctggt gaaaatcaaa gtcctggagc aagacaaggc
     8401 aaggctgcag aggctggagg atgagctgaa tcgtgcaaaa tcaactctag aggcagaaac
     8461 cagggtgaaa cagcgcctgg agtgtgagaa acagcaaatt cagaatgacc tgaatcagtg
     8521 gaagactcaa tattcccgca aggaggaggc tattaggaag atagaatcgg aaagagaaaa
     8581 gagtgagaga gagaagaaca gtcttaggag tgagatcgaa agactccaag cagagatcaa
     8641 gagaattgaa gagaggtgca ggcgtaagct ggaggattct accagggaga cacagtcaca
     8701 gttagaaaca gaacgctccc gatatcagag ggagattgat aaactcagac agcgcccata
     8761 tgggtcccat cgagaggtcg agatggtgag caaaggcgaa gagctgttca ccggcgtggt
     8821 gcccatcctg gtggagctgg acggcgacgt gaacggccac aagttcagcg tgagcggcga
     8881 gggcgagggc gacgccacct acggcaagct gaccctgaag ctgctgtgca ccaccggcaa
     8941 gctgcccgtg ccctggccca ccctggtgac caccctgggc tacggcgtgc agtgcttcgc
     9001 ccggtacccc gaccacatga agcagcacga cttcttcaag agcgccatgc ccgagggcta
     9061 cgtgcaggag cggaccatct tcttcaagga cgacggcaac tacaagaccc gggccgaggt
     9121 gaagttcgag ggcgacaccc tggtgaaccg gatcgagctg aagggcatcg acttcaagga
     9181 ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca acgtgtacat
     9241 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccggc acaacatcga
     9301 ggacggcggc gtgcagctgg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     9361 cgtgctgctg cccgacaacc actacctgag ctaccagagc gccctgttca aggaccccaa
     9421 cgagaagcgg gaccacatgg tgctgctgga gttcctgacc gccgccggat ccgggccagg
     9481 tggagcaggg ccaggtggtg cagggccagg tggtgcaggg ccaggtggtg cagggcccgg
     9541 tggtgcaggt ccaggtggtg caggtccagg tggtgctggt ccaggtggtg ctggatccat
     9601 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     9661 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     9721 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     9781 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     9841 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     9901 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     9961 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
    10021 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
    10081 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
    10141 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
    10201 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
    10261 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaaggcgg ccgctaccca
    10321 gactgagtgt gagtggaccg ttgacacctc caagctggtg tttgatgggc tgaggaagaa
    10381 ggtgacagca atgcagctct atgagtgtca gctgatcgac aaaacaacct tggacaaact
    10441 attgaagggg aagaagtcag tggaagaagt tgcttctgaa atccagccat tccttcgggg
    10501 tgcaggatct atcgctggag catctgcttc tcctaaggaa aaatactctt tggtagaggc
    10561 caagagaaag aaattaatca gcccagaatc cacagtcatg cttctggagg cccaggcagc
    10621 tacaggtggt ataattgatc cccatcggaa tgagaagctg actgtggaca gtgccatagc
    10681 tcgggacctc attgacttcg atgaccgtca gcagatatat gcagcagaaa aagctatcac
    10741 tggttttgat gatccatttt caggcaagac agtatctgtt tcagaagcca tcaagaaaaa
    10801 tttgattgat agagaaaccg gaatgcgcct gctggaagcc cagattgctt cagggggtgt
    10861 agtagaccct gtgaacagtg tctttttgcc aaaagatgtc gccttggccc gggggctgat
    10921 tgatagagat ttgtatcgtt ccctgaatga tccccgagat agtcagaaaa actttgtgga
    10981 tccagtcacc aaaaagaagg tcagttacgt gcagctgaag gaacggtgca gaatcgaacc
    11041 acatactggt ctgctcttgc tttcagtaca gaagagaagc atgtccttcc aaggaatcag
    11101 acaacctgtg accgtcactg agctagtaga ttctggtata ttgagaccgt ccactgtcaa
    11161 tgaactggaa tctggtcaga tttcttatga cgaggttggt gagagaatta aggacttcct
    11221 ccagggttca agctgcatag caggcatata caatgagacc acaaaacaga agcttggcat
    11281 ttatgaggcc atgaaaattg gcttagtccg acctggtact gctctggagt tgctggaagc
    11341 ccaagcagct actggcttta tagtggatcc tgttagcaac ttgaggttac cagtggagga
    11401 agcctacaag agaggtctgg tgggcattga gttcaaagag aagctcctgt ctgcagaacg
    11461 agctgtcact gggtataatg atcctgaaac aggaaacatc atctctttgt tccaagccat
    11521 gaataaggaa ctcatcgaaa agggccacgg tattcgctta ttagaagcac agatcgcaac
    11581 cggggggatc attgacccaa aggagagcca tcgtttacca gttgacatag catataagag
    11641 gggctatttc aatgaggaac tcagtgagat tctctcagat ccaagtgatg ataccaaagg
    11701 attttttgac cccaacactg aagaaaatct tacctatctg caactaaaag aaagatgcat
    11761 taaggatgag gaaacagggc tctgtcttct gcctctgaaa gaaaagaaga aacaggtgca
    11821 gacatcacaa aagaataccc tcaggaagcg tagagtggtc atagttgacc cagaaaccaa
    11881 taaagaaatg tctgttcagg aggcctacaa gaagggccta attgattatg aaaccttcaa
    11941 agaactgtgt gagcaggaat gtgaatggga agaaataacc atcacgggat cagatggctc
    12001 caccagggtg gtcctggtag atagaaagac aggcagtcag tatgatattc aagatgctat
    12061 tgacaagggc cttgttgaca ggaagttctt tgatcagtac cgatccggca gcctcagcct
    12121 cactcaattt gctgacatga tctccttgaa aaatggtgtc ggcaccagca gcagcatggg
    12181 cagtggtgtc agcgatgatg tttttagcag ctcccgacat gaatcagtaa gtaagatttc
    12241 caccatatcc agcgtcagga atttaaccat aaggagcagc tctttttcag acaccctgga
    12301 agaatcgagc cccattgcag ccatctttga cacagaaaac ctggagaaaa tctccattac
    12361 agaaggtata gagcggggca tcgttgacag catcacgggt cagaggcttc tggaggctca
    12421 ggcctgcaca ggtggcatca tccacccaac cacgggccag aagctgtcac ttcaggacgc
    12481 agtctcccag ggtgtgattg accaagacat ggccaccagg ctgaagcctg ctcagaaagc
    12541 cttcataggc ttcgagggtg tgaagggaaa gaagaagatg tcagcagcag aggcagtgaa
    12601 agaaaaatgg ctcccgtatg aggctggcca gcgcttcctg gagttccagt acctcacggg
    12661 aggtcttgtt gacccggaag tgcatgggag gataagcacc gaagaagcca tccggaaggg
    12721 gttcatagat ggccgcgccg cacagaggct gcaagacacc agcagctatg ccaaaatcct
    12781 gacctgcccc aaaaccaaat taaaaatatc ctataaggat gccataaatc gctccatggt
    12841 agaagatatc actgggctgc gccttctgga agccgcctcc gtgtcgtcca agggcttacc
    12901 cagcccttac aacatgtctt cggctccggg gtcccgctcc ggctcccgct cgggatctcg
    12961 ctccggatct cgctccgggt cccgcagtgg gtcccggaga ggaagctttg acgccacagg
    13021 caattcttcc tactcttatt cctactcatt tagcagtagt tctattgggc actaaatcga
    13081 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
    13141 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga
    13201 gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca
    13261 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg
    13321 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
    13381 cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc
    13441 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg
    13501 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc
    13561 tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa
    13621 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
    13681 gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac
    13741 ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggctgcctc gcgcgtttcg
    13801 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
    13861 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
    13921 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtactgataa cttcgtataa
    13981 tgtatgctat acgaagttat taggtctgaa gaggagttta cgtccagcca agctagggcc
    14041 gcgatccgga acccttaata taacttcgta taatgtatgc tatacgaagt tatcagtact
    14101 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
    14161 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
    14221 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    14281 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    14341 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    14401 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    14461 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    14521 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    14581 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    14641 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    14701 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    14761 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    14821 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    14881 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    14941 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    15001 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    15061 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    15121 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    15181 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    15241 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    15301 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    15361 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    15421 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct
    15481 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    15541 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    15601 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    15661 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    15721 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
    15781 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    15841 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    15901 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    15961 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    16021 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    16081 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
    16141 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
    16201 gtcttcaaga attgctagca attgctagca attgctagca attcatacca gatcaccgaa
    16261 aactgtcctc caaatgtgtc cccctcacac tcccaaattc gcgggcttct gcctcttaga
    16321 ccactctacc ctattcccca cactcaccgg agccaaagcc gcggcccttc cgtttctttg
    16381 ct
//
