LOCUS       sequence-230442-       14708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The donor only (YPet(short)) control for the FL-based human 
            desmoplakin II tension sensor provides the donor only lifetime used 
            in fluorescence lifetime measurements to determine FRET..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14708)
  AUTHORS   Price AJ, Cost AL, Ungewiss H, Waschke J, Dunn AR, Grashoff C
  TITLE     Mechanical loading of desmosomes depends on the magnitude and 
            orientation of external stress.
  JOURNAL   Nat Commun. 2018 Dec 11;9(1):5284. doi: 10.1038/s41467-018-07523-0.
  PUBMED    30538252
REFERENCE   2  (bases 1 to 14708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-11"; volume: "9(1):5284. doi"; pages: " 
            10.1038/s41467-018-07523-0"
COMMENT     Sequence Label: Desmoplakin II donor only control (FL-based te...
FEATURES             Location/Qualifiers
     source          1..14708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             414..589
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    652..851
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1052..1468
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1398..1420
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1436..1452
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1565..2164
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1565..1584)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2061..2081
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     enhancer        2339..2642
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2643..2846
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2797..2817
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(7289..7308)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             7673..7918
                     /codon_start=1
                     /product="mechanically labile synthetic ferredoxin-like 
                     domain (Fang et al., 2013)"
                     /label=FL
                     /note="can serve as an intracellular tension sensor (Ringer
                     et al., 2017)"
                     /translation="MGEFDIRFRTDDDEQFEKVLKEMNRRARKDAGTVTYTRDGNDFEI
                     RITGISEQNRKELAKEVERLAKEQNITVTYTERGSLE"
     CDS             7925..8632
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMLGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8070..8088)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8338..8357
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             11447..12040
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(12143..12165)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    12293..12326
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    12386..12419
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     12439..12458
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12539..12556)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12710..13298)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12790..12809)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13469..14260,14261..14329))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14092..14111
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14330..14434)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     14502..14520
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
     1441 tccagccctc actccttctc taggcgccgg aatgatcccc cgggctgcag gtcggccgcc
     1501 acgaccggtg ccgccaccat cccctgaccc acgcccctga cccctcacaa ggagacgacc
     1561 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     1621 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     1681 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     1741 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     1801 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     1861 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     1921 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     1981 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     2041 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     2101 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     2161 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatggctc
     2221 cgaccgaagc cacccggggc ggccccgccg accccgcacc cgcccccgag gcccaccgac
     2281 tctagtaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     2341 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     2401 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     2461 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     2521 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     2581 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     2641 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     2701 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     2761 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     2821 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
     2881 ggactcagat ctgggcccat aaagcttata cgaattcatg agctgcaacg gaggctccca
     2941 cccgcggatc aacactctgg gccgcatgat ccgcgccgag tctggcccgg acctgcgcta
     3001 cgaggtgacc agcggcggcg ggggcaccag caggatgtac tattctcggc gcggcgtgat
     3061 caccgaccag aactcggacg gctactgtca aaccggcacg atgtccaggc accagaacca
     3121 gaacaccatc caggagctgc tgcagaactg ctccgactgc ttgatgcgag cagagctcat
     3181 cgtgcagcct gaattgaagt atggagatgg aatacaactg actcggagtc gagaattgga
     3241 tgagtgtttt gcccaggcca atgaccaaat ggaaatcctc gacagcttga tcagagagat
     3301 gcggcagatg ggccagccct gtgatgctta ccagaaaagg cttcttcagc tccaagagca
     3361 aatgcgagcc ctttataaag ccatcagtgt cccacgagtc cgcagggcca gctccaaggg
     3421 tggtggaggc tacacttgtc agagtggctc tggctgggat gagttcacca aacatgtcac
     3481 cagtgaatgt ttggggtgga tgaggcagca aagggcggag atggacatgg tggcctgggg
     3541 tgtggacctg gcctcagtgg agcagcacat taacagccac cggggcatcc acaactccat
     3601 cggcgactat cgctggcagc tggacaaaat caaagccgac ctgcgcgaga aatctgcgat
     3661 ctaccagttg gaggaggagt atgaaaacct gctgaaagcg tcctttgaga ggatggatca
     3721 cctgcgacag ctgcagaaca tcattcaggc cacgtccagg gagatcatgt ggatcaatga
     3781 ctgcgaggag gaggagctgc tgtacgactg gagcgacaag aacaccaaca tcgctcagaa
     3841 acaggaggcc ttctccatac gcatgagtca actggaagtt aaagaaaaag agctcaataa
     3901 gctgaaacaa gaaagtgacc aacttgtcct caatcagcat ccagcttcag acaaaattga
     3961 ggcctatatg gacactctgc agacgcagtg gagttggatt cttcagatca ccaagtgcat
     4021 tgatgttcat ctgaaagaaa atgctgccta ctttcagttt tttgaagagg cgcagtctac
     4081 tgaagcatac ctgaaggggc tccaggactc catcaggaag aagtacccct gcgacaagaa
     4141 catgcccctg cagcacctgc tggaacagat caaggagctg gagaaagaac gagagaaaat
     4201 ccttgaatac aagcgtcagg tgcagaactt ggtaaacaag tctaagaaga ttgtacagct
     4261 gaagcctcgt aacccagact acagaagcaa taaacccatt attctcagag ctctctgtga
     4321 ctacaaacaa gatcagaaaa tcgtgcataa gggggatgag tgtatcctga aggacaacaa
     4381 cgagcgcagc aagtggtacg tgacgggccc gggaggcgtt gacatgcttg ttccctctgt
     4441 ggggctgatc atccctcctc cgaacccact ggccgtggac ctctcttgca agattgagca
     4501 gtactacgaa gccatcttgg ctctgtggaa ccagctctac atcaacatga agagcctggt
     4561 gtcctggcac tactgcatga ttgacataga gaagatcagg gccatgacaa tcgccaagct
     4621 gaaaacaatg cggcaggaag attacatgaa gacgatagcc gaccttgagt tacattacca
     4681 agagttcatc agaaatagcc aaggctcaga gatgtttgga gatgatgaca agcggaaaat
     4741 acagtctcag ttcaccgatg cccagaagca ttaccagacc ctggtcattc agctccctgg
     4801 ctatccccag caccagacag tgaccacaac tgaaatcact catcatggaa cctgccaaga
     4861 tgtcaaccat aataaagtaa ttgaaaccaa cagagaaaat gacaagcaag aaacatggat
     4921 gctgatggag ctgcagaaga ttcgcaggca gatagagcac tgcgagggca ggatgactct
     4981 caaaaacctc cctctagcag accaggggtc ttctcaccac atcacagtga aaattaacga
     5041 gcttaagagt gtgcagaatg attcacaagc aattgctgag gttctcaacc agcttaaaga
     5101 tatgcttgcc aacttcagag gttctgaaaa gtactgctat ttacagaatg aagtatttgg
     5161 actatttcag aaactggaaa atatcaatgg tgttacagat ggctacttaa atagcttatg
     5221 cacagtaagg gcactgctcc aggctattct ccaaacagaa gacatgttaa aggtttatga
     5281 agccaggctc actgaggagg aaactgtctg cctggacctg gataaagtgg aagcttaccg
     5341 ctgtggactg aagaaaataa aaaatgactt gaacttgaag aagtcgttgt tggccactat
     5401 gaagacagaa ctacagaaag cccagcagat ccactctcag acttcacagc agtatccact
     5461 ttatgatctg gacttgggca agttcggtga aaaagtcaca cagctgacag accgctggca
     5521 aaggatagat aaacagatcg actttagatt atgggacctg gagaaacaaa tcaagcaatt
     5581 gaggaattat cgtgataact atcaggcttt ctgcaagtgg ctctatgatg ctaaacgccg
     5641 ccaggattcc ttagaatcca tgaaatttgg agattccaac acagtcatgc ggtttttgaa
     5701 tgagcagaag aacttgcaca gtgaaatatc tggcaaacga gacaaatcag aggaagtaca
     5761 aaaaattgct gaactttgcg ccaattcaat taaggattat gagctccagc tggcctcata
     5821 cacctcagga ctggaaactc tgctgaacat acctatcaag aggaccatga ttcagtcccc
     5881 ttctggggtg attctgcaag aggctgcaga tgttcatgct cggtacattg aactacttac
     5941 aagatctgga gactattaca ggttcttaag tgagatgctg aagagtttgg aagatctgaa
     6001 gctgaaaaat accaagatcg aagttttgga agaggagctc agactggccc gagatgccaa
     6061 ctcggaaaac tgtaataaga acaaattcct ggatcagaac ctgcagaaat accaggcaga
     6121 gtgttcccag ttcaaagcga agcttgcgag cctggaggag ctgaagagac aggctgagct
     6181 ggatgggaag tcggctaagc aaaatctaga caagtgctac ggccaaataa aagaactcaa
     6241 tgagaagatc acccgactga cttatgagat tgaagatgaa aagagaagaa gaaaatctgt
     6301 ggaagacaga tttgaccaac agaagaatga ctatgaccaa ctgcagaaag caaggcaatg
     6361 tgaaaaggag aaccttggtt ggcagaaatt agagtctgag aaagccatca aggagaagga
     6421 gtacgagatt gaaaggttga gggttctact gcaggaagaa ggcacccgga agagagaata
     6481 tgaaaatgag ctggcaaagg catctaatag gattcaggaa tcaaagaatc agtgtactca
     6541 ggtggtacag gaaagagaga gccttctggt gaaaatcaaa gtcctggagc aagacaaggc
     6601 aaggctgcag aggctggagg atgagctgaa tcgtgcaaaa tcaactctag aggcagaaac
     6661 cagggtgaaa cagcgcctgg agtgtgagaa acagcaaatt cagaatgacc tgaatcagtg
     6721 gaagactcaa tattcccgca aggaggaggc tattaggaag atagaatcgg aaagagaaaa
     6781 gagtgagaga gagaagaaca gtcttaggag tgagatcgaa agactccaag cagagatcaa
     6841 gagaattgaa gagaggtgca ggcgtaagct ggaggattct accagggaga cacagtcaca
     6901 gttagaaaca gaacgctccc gatatcagag ggagattgat aaactcagac agcgcccata
     6961 tgggtcccat cgagaggtcg agatggtgag caaaggcgaa gagctgttca ccggcgtggt
     7021 gcccatcctg gtggagctgg acggcgacgt gaacggccac aagttcagcg tgagcggcga
     7081 gggcgagggc gacgccacct acggcaagct gaccctgaag ctgctgtgca ccaccggcaa
     7141 gctgcccgtg ccctggccca ccctggtgac caccctgggc tacggcgtgc agtgcttcgc
     7201 ccggtacccc gaccacatga agcagcacga cttcttcaag agcgccatgc ccgagggcta
     7261 cgtgcaggag cggaccatct tcttcaagga cgacggcaac tacaagaccc gggccgaggt
     7321 gaagttcgag ggcgacaccc tggtgaaccg gatcgagctg aagggcatcg acttcaagga
     7381 ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca acgtgtacat
     7441 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccggc acaacatcga
     7501 ggacggcggc gtgcagctgg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     7561 cgtgctgctg cccgacaacc actacctgag ctaccagagc gccctgttca aggaccccaa
     7621 cgagaagcgg gaccacatgg tgctgctgga gttcctgacc gccgccggat ctatgggcga
     7681 gtttgacatc cggtttcgga ctgatgacga cgaacagttc gagaaagtgc tgaaggagat
     7741 gaatcgtcga gccagaaagg atgctggaac tgtgacctac acaagggatg ggaatgactt
     7801 cgagattcgc attaccggca taagcgagca aaaccgcaaa gaactggcca aagaggttga
     7861 aaggcttgca aaggaacaga acatcacagt cacgtatacc gagagaggtt ccctcgaagg
     7921 atccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     7981 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     8041 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     8101 gcccttcgcc tgggacatcc tgtcccctca gttcatgctc ggctccaagg cctacgtgaa
     8161 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     8221 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     8281 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     8341 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     8401 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     8461 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     8521 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     8581 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca aggcggccgc
     8641 tacccagact gagtgtgagt ggaccgttga cacctccaag ctggtgtttg atgggctgag
     8701 gaagaaggtg acagcaatgc agctctatga gtgtcagctg atcgacaaaa caaccttgga
     8761 caaactattg aaggggaaga agtcagtgga agaagttgct tctgaaatcc agccattcct
     8821 tcggggtgca ggatctatcg ctggagcatc tgcttctcct aaggaaaaat actctttggt
     8881 agaggccaag agaaagaaat taatcagccc agaatccaca gtcatgcttc tggaggccca
     8941 ggcagctaca ggtggtataa ttgatcccca tcggaatgag aagctgactg tggacagtgc
     9001 catagctcgg gacctcattg acttcgatga ccgtcagcag atatatgcag cagaaaaagc
     9061 tatcactggt tttgatgatc cattttcagg caagacagta tctgtttcag aagccatcaa
     9121 gaaaaatttg attgatagag aaaccggaat gcgcctgctg gaagcccaga ttgcttcagg
     9181 gggtgtagta gaccctgtga acagtgtctt tttgccaaaa gatgtcgcct tggcccgggg
     9241 gctgattgat agagatttgt atcgttccct gaatgatccc cgagatagtc agaaaaactt
     9301 tgtggatcca gtcaccaaaa agaaggtcag ttacgtgcag ctgaaggaac ggtgcagaat
     9361 cgaaccacat actggtctgc tcttgctttc agtacagaag agaagcatgt ccttccaagg
     9421 aatcagacaa cctgtgaccg tcactgagct agtagattct ggtatattga gaccgtccac
     9481 tgtcaatgaa ctggaatctg gtcagatttc ttatgacgag gttggtgaga gaattaagga
     9541 cttcctccag ggttcaagct gcatagcagg catatacaat gagaccacaa aacagaagct
     9601 tggcatttat gaggccatga aaattggctt agtccgacct ggtactgctc tggagttgct
     9661 ggaagcccaa gcagctactg gctttatagt ggatcctgtt agcaacttga ggttaccagt
     9721 ggaggaagcc tacaagagag gtctggtggg cattgagttc aaagagaagc tcctgtctgc
     9781 agaacgagct gtcactgggt ataatgatcc tgaaacagga aacatcatct ctttgttcca
     9841 agccatgaat aaggaactca tcgaaaaggg ccacggtatt cgcttattag aagcacagat
     9901 cgcaaccggg gggatcattg acccaaagga gagccatcgt ttaccagttg acatagcata
     9961 taagaggggc tatttcaatg aggaactcag tgagattctc tcagatccaa gtgatgatac
    10021 caaaggattt tttgacccca acactgaaga aaatcttacc tatctgcaac taaaagaaag
    10081 atgcattaag gatgaggaaa cagggctctg tcttctgcct ctgaaagaaa agaagaaaca
    10141 ggtgcagaca tcacaaaaga ataccctcag gaagcgtaga gtggtcatag ttgacccaga
    10201 aaccaataaa gaaatgtctg ttcaggaggc ctacaagaag ggcctaattg attatgaaac
    10261 cttcaaagaa ctgtgtgagc aggaatgtga atgggaagaa ataaccatca cgggatcaga
    10321 tggctccacc agggtggtcc tggtagatag aaagacaggc agtcagtatg atattcaaga
    10381 tgctattgac aagggccttg ttgacaggaa gttctttgat cagtaccgat ccggcagcct
    10441 cagcctcact caatttgctg acatgatctc cttgaaaaat ggtgtcggca ccagcagcag
    10501 catgggcagt ggtgtcagcg atgatgtttt tagcagctcc cgacatgaat cagtaagtaa
    10561 gatttccacc atatccagcg tcaggaattt aaccataagg agcagctctt tttcagacac
    10621 cctggaagaa tcgagcccca ttgcagccat ctttgacaca gaaaacctgg agaaaatctc
    10681 cattacagaa ggtatagagc ggggcatcgt tgacagcatc acgggtcaga ggcttctgga
    10741 ggctcaggcc tgcacaggtg gcatcatcca cccaaccacg ggccagaagc tgtcacttca
    10801 ggacgcagtc tcccagggtg tgattgacca agacatggcc accaggctga agcctgctca
    10861 gaaagccttc ataggcttcg agggtgtgaa gggaaagaag aagatgtcag cagcagaggc
    10921 agtgaaagaa aaatggctcc cgtatgaggc tggccagcgc ttcctggagt tccagtacct
    10981 cacgggaggt cttgttgacc cggaagtgca tgggaggata agcaccgaag aagccatccg
    11041 gaaggggttc atagatggcc gcgccgcaca gaggctgcaa gacaccagca gctatgccaa
    11101 aatcctgacc tgccccaaaa ccaaattaaa aatatcctat aaggatgcca taaatcgctc
    11161 catggtagaa gatatcactg ggctgcgcct tctggaagcc gcctccgtgt cgtccaaggg
    11221 cttacccagc ccttacaaca tgtcttcggc tccggggtcc cgctccggct cccgctcggg
    11281 atctcgctcc ggatctcgct ccgggtcccg cagtgggtcc cggagaggaa gctttgacgc
    11341 cacaggcaat tcttcctact cttattccta ctcatttagc agtagttcta ttgggcacta
    11401 aatcgataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca
    11461 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat
    11521 aactgagaat agagaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc
    11581 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa
    11641 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
    11701 caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga
    11761 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc
    11821 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac
    11881 aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc
    11941 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc
    12001 ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg
    12061 ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc tgcctcgcgc
    12121 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
    12181 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
    12241 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtac tgataacttc
    12301 gtataatgta tgctatacga agttattagg tctgaagagg agtttacgtc cagccaagct
    12361 agggccgcga tccggaaccc ttaatataac ttcgtataat gtatgctata cgaagttatc
    12421 agtactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
    12481 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
    12541 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    12601 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    12661 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    12721 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    12781 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    12841 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    12901 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    12961 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    13021 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    13081 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    13141 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    13201 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    13261 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    13321 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    13381 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    13441 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    13501 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    13561 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    13621 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    13681 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    13741 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
    13801 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    13861 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    13921 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    13981 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    14041 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
    14101 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    14161 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    14221 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    14281 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    14341 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    14401 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    14461 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
    14521 cctttcgtct tcaagaattg ctagcaattg ctagcaattg ctagcaattc ataccagatc
    14581 accgaaaact gtcctccaaa tgtgtccccc tcacactccc aaattcgcgg gcttctgcct
    14641 cttagaccac tctaccctat tccccacact caccggagcc aaagccgcgg cccttccgtt
    14701 tctttgct
//
