LOCUS       sequence-230449-        9295 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express 3xflag tagged ZFP64.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9295)
  AUTHORS   Lu B, Klingbeil O, Tarumoto Y, Somerville TDD, Huang YH, Wei Y, Wai 
            DC, Low JKK, Milazzo JP, Wu XS, Cao Z, Yan X, Demerdash OE, Huang G,
            Mackay JP, Kinney JB, Shi J, Vakoc CR
  TITLE     A Transcription Factor Addiction in Leukemia Imposed by the MLL 
            Promoter Sequence.
  JOURNAL   Cancer Cell. 2018 Dec 10;34(6):970-981.e8. doi: 
            10.1016/j.ccell.2018.10.015. Epub 2018 Nov 29.
  PUBMED    30503706
REFERENCE   2  (bases 1 to 9295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.10.015"; journalName: "Cancer Cell"; date: 
            "2018-12-10- 10"; volume: "34"; issue: "6"; pages: "970-981.e8"
COMMENT     Sequence Label: Lenti_Neo_ZFP64_N_3XFLAG
FEATURES             Location/Qualifiers
     source          1..9295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..212
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      232..241
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             241..306
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2359..2415
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2434..3228
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2488..2507)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3098..3117
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    3244..3832
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3297..3317)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3715..3726)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3904..4137
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4215..4336
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4252..4271)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4306..4325
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4376..4511
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4531..4550)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4532..4550)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4560..4577)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4560..4576)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4569..4591)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4718..5173
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4805..4824)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5015..5036
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5199..5303
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5304..5372,5373..6164)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5522..5541)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6335..6923
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6824..6843
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7077..7094
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7211..7232
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7247..7252,7253..7270,7271..7277)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7285..7301
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7290..7312
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7309..7325
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7309..7325
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7344..7364
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7346..7364
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7392..7618
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7619..7799
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7846..7971
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8464..8697
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8882..8926
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9110..9227
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
       61 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
      121 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
      181 tttttcgcaa cgggtttgcc gccagaacac aggtgtcgtg acgcgggatc cgccaccatg
      241 gattacaaag accatgacgg tgattataaa gatcatgata tcgattacaa ggatgacgat
      301 gacaagatga acgcgagcag cgagggcgag agcttcgcgg gctcggtgca aattccaggt
      361 ggcacaacgg tgctggtgga gctgactccc gacatccata tctgcggcat ctgcaagcag
      421 cagtttaaca acctggatgc ctttgtagct cacaagcaaa gtggctgcca gctgacaggc
      481 acatccgcag cagcccccag cacggtccag tttgtatcgg aggaaacagt gcctgccacc
      541 cagactcaga ccaccaccag aaccatcacc tcggagaccc agacaatcac agtttcagct
      601 ccagaatttg tttttgaaca tggctatcaa acttacctgc ccacggaaag taatgaaaac
      661 cagacagcca ctgtcatctc tctccctgcc aagtcacgca ccaaaaagcc cacaacacca
      721 cctgctcaga aaaggcttaa ctgttgctat ccaggttgcc aattcaagac tgcttatggc
      781 atgaaggaca tggagcggca tttaaaaatt cacacgggag acaaacccca taagtgtgaa
      841 gtctgtggca agtgctttag ccggaaagac aagctgaaaa ctcacatgcg gtgccacacg
      901 ggcgtgaagc cctacaagtg taagacgtgt gactacgccg ctgccgacag cagcagcctc
      961 aacaagcacc tgaggatcca ctcggacgag cggcccttca aatgccagat ctgcccctac
     1021 gccagccgca actccagcca gctcactgtc cacctgcgat cccacacggg ggacgccccc
     1081 ttccagtgct ggctctgtag cgccaagttc aaaatcagct cggacttgaa aaggcacatg
     1141 cgggtgcact cgggggagaa gcctttcaag tgcgagttct gcaatgtccg ctgcaccatg
     1201 aaggggaacc tcaagtcgca catccgtatc aagcacagcg ggaataactt caagtgtcct
     1261 cattgcgact tcctgggtga cagcaaagcc accctccgga agcacagccg cgtgcaccag
     1321 tcggagcatc ctgagaagtg ctcggaatgc agctactcct gctccagcaa ggccgccctg
     1381 cgcatccacg agcgtatcca ctgcaccgac cgccctttca agtgcaacta ctgcagcttc
     1441 gacaccaaac agcccagcaa cctgagcaag cacatgaaga agttccacgg ggacatggtt
     1501 aagactgagg ctctagagag gaaggacacc ggcaggcaga gcagccggca ggtggccaag
     1561 ctggatgcca agaagagttt ccactgcgat atatgcgatg cctccttcat gcgggaggac
     1621 tcgctccgca gccacaagag acagcacagt gagtacagtg agagtaagaa ctcggacgtg
     1681 accgttctcc agtttcagat cgaccccagc aagcagcccg ccacgcccct cactgtggga
     1741 cacctccagg tgcccctcca gcccagccaa gtgccccagt tcagcgaggg aagagtcaaa
     1801 atcatcgttg ggcatcaggt gccccaggcg aacaccatcg tccaggctgc cgctgctgca
     1861 gtgaacatcg tcccgcctgc cttggtggcc cagaacccag aggaactccc agggaacagc
     1921 cggctgcaga tcctgcgcca ggtcagtctg atcgcccccc ctcagtcctc gcggtgtccg
     1981 agcgaggcgg gcgcaatgac ccagccggct gtcctgctga ccacccacga gcagacggac
     2041 ggagccactc tgcaccagac tctcatcccc acggcctcag gtggccccca ggaaggctct
     2101 ggcaatcaaa ctttcattac cagttcgggt attacttgca ctgactttga aggcctaaac
     2161 gccttgattc aggaggggac agcagaagtg acagtggtga gcgatggagg ccagaacatc
     2221 gcagtggcca ccacagcgcc accggtcttc tcctcctctt cccagcaaga actacccaag
     2281 cagacctact ccatcattca aggggcagcc catccagctt tgctctgtcc cgccgactcc
     2341 attccagatg gcagcggcgc caccaacttc agcctgctga agcaggccgg cgacgtggag
     2401 gagaaccccg gccccatgac cgagtacaag cccatgattg aacaagatgg attgcacgca
     2461 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     2521 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     2581 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     2641 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     2701 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     2761 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     2821 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     2881 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     2941 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     3001 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     3061 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     3121 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     3181 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaac gcgttaagtc
     3241 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3301 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3361 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3421 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3481 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     3541 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3601 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     3661 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     3721 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3781 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     3841 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     3901 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     3961 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     4021 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     4081 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     4141 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     4201 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     4261 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     4321 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     4381 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     4441 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     4501 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     4561 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     4621 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     4681 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     4741 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4801 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4861 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4921 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4981 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5041 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5101 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     5161 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5221 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5281 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5341 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5401 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5461 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5521 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5581 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5641 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5701 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5761 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5821 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5881 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     5941 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6001 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6061 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6121 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6181 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6241 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6301 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6361 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6421 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6481 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6541 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6601 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6661 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6721 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6781 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6841 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6901 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6961 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7021 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7081 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     7141 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     7201 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     7261 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     7321 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     7381 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     7441 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     7501 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     7561 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     7621 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7681 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7741 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7801 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     7861 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     7921 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     7981 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     8041 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     8101 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     8161 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     8221 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     8281 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     8341 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     8401 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     8461 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     8521 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     8581 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     8641 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     8701 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     8761 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     8821 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     8881 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     8941 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     9001 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     9061 attatcgttt cagacccacc tcccaacccc gaggggaccc agaccacaat tttaaaagaa
     9121 aaggggggat tgggaggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     9181 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     9241 gggacagcag agatccactt tggcgccggc tcgagtggct ccggtgcccg tcagt
//
