LOCUS       sequence-230450-        9691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express 3xflag tagged ZFP64 for flag ChIP experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   Lu B, Klingbeil O, Tarumoto Y, Somerville TDD, Huang YH, Wei Y, Wai 
            DC, Low JKK, Milazzo JP, Wu XS, Cao Z, Yan X, Demerdash OE, Huang G,
            Mackay JP, Kinney JB, Shi J, Vakoc CR
  TITLE     A Transcription Factor Addiction in Leukemia Imposed by the MLL 
            Promoter Sequence.
  JOURNAL   Cancer Cell. 2018 Dec 10;34(6):970-981.e8. doi: 
            10.1016/j.ccell.2018.10.015. Epub 2018 Nov 29.
  PUBMED    30503706
REFERENCE   2  (bases 1 to 9691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.10.015"; journalName: "Cancer Cell"; date: 
            "2018-12-10- 10"; volume: "34"; issue: "6"; pages: "970-981.e8"
COMMENT     Sequence Label: pHAGE_puro_ZFP64_N_3XFLAG
FEATURES             Location/Qualifiers
     source          1..9691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             292..357
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        2418..2917
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2453..2476)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2850..2869
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2938..3537
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2938..2957)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3434..3454
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3551..4139
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3604..3624)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4022..4033)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4214..4447
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4566..4584)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4652..4756
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4757..4825,4826..5617)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4975..4994)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5788..6376
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6277..6296
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6530..6547
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6787..6808
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6823..6828,6829..6846,6847..6853)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6861..6877
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6866..6888
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6885..6901
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6885..6901
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6951..6970
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7162..7795
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7842..7967
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8464..8697
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8882..8926
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9219..9336
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        9388..9691
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cggcggccac gcgtcaccat ggattacaaa
      301 gaccatgacg gtgattataa agatcatgat atcgattaca aggatgacga tgacaagatg
      361 aacgcgagca gcgagggcga gagcttcgcg ggctcggtgc aaattccagg tggcacaacg
      421 gtgctggtgg agctgactcc cgacatccat atctgcggca tctgcaagca gcagtttaac
      481 aacctggatg cctttgtagc tcacaagcaa agtggctgcc agctgacagg cacatccgca
      541 gcagccccca gcacggtcca gtttgtatcg gaggaaacag tgcctgccac ccagactcag
      601 accaccacca gaaccatcac ctcggagacc cagacaatca cagtttcagc tccagaattt
      661 gtttttgaac atggctatca aacttacctg cccacggaaa gtaatgaaaa ccagacagcc
      721 actgtcatct ctctccctgc caagtcacgc accaaaaagc ccacaacacc acctgctcag
      781 aaaaggctta actgttgcta tccaggttgc caattcaaga ctgcttatgg catgaaggac
      841 atggagcggc atttaaaaat tcacacggga gacaaacccc ataagtgtga agtctgtggc
      901 aagtgcttta gccggaaaga caagctgaaa actcacatgc ggtgccacac gggcgtgaag
      961 ccctacaagt gtaagacgtg tgactacgcc gctgccgaca gcagcagcct caacaagcac
     1021 ctgaggatcc actcggacga gcggcccttc aaatgccaga tctgccccta cgccagccgc
     1081 aactccagcc agctcactgt ccacctgcga tcccacacgg gggacgcccc cttccagtgc
     1141 tggctctgta gcgccaagtt caaaatcagc tcggacttga aaaggcacat gcgggtgcac
     1201 tcgggggaga agcctttcaa gtgcgagttc tgcaatgtcc gctgcaccat gaaggggaac
     1261 ctcaagtcgc acatccgtat caagcacagc gggaataact tcaagtgtcc tcattgcgac
     1321 ttcctgggtg acagcaaagc caccctccgg aagcacagcc gcgtgcacca gtcggagcat
     1381 cctgagaagt gctcggaatg cagctactcc tgctccagca aggccgccct gcgcatccac
     1441 gagcgtatcc actgcaccga ccgccctttc aagtgcaact actgcagctt cgacaccaaa
     1501 cagcccagca acctgagcaa gcacatgaag aagttccacg gggacatggt taagactgag
     1561 gctctagaga ggaaggacac cggcaggcag agcagccggc aggtggccaa gctggatgcc
     1621 aagaagagtt tccactgcga tatatgcgat gcctccttca tgcgggagga ctcgctccgc
     1681 agccacaaga gacagcacag tgagtacagt gagagtaaga actcggacgt gaccgttctc
     1741 cagtttcaga tcgaccccag caagcagccc gccacgcccc tcactgtggg acacctccag
     1801 gtgcccctcc agcccagcca agtgccccag ttcagcgagg gaagagtcaa aatcatcgtt
     1861 gggcatcagg tgccccaggc gaacaccatc gtccaggctg ccgctgctgc agtgaacatc
     1921 gtcccgcctg ccttggtggc ccagaaccca gaggaactcc cagggaacag ccggctgcag
     1981 atcctgcgcc aggtcagtct gatcgccccc cctcagtcct cgcggtgtcc gagcgaggcg
     2041 ggcgcaatga cccagccggc tgtcctgctg accacccacg agcagacgga cggagccact
     2101 ctgcaccaga ctctcatccc cacggcctca ggtggccccc aggaaggctc tggcaatcaa
     2161 actttcatta ccagttcggg tattacttgc actgactttg aaggcctaaa cgccttgatt
     2221 caggagggga cagcagaagt gacagtggtg agcgatggag gccagaacat cgcagtggcc
     2281 accacagcgc caccggtctt ctcctcctct tcccagcaag aactacccaa gcagacctac
     2341 tccatcattc aaggggcagc ccatccagct ttgctctgtc ccgccgactc cattccagat
     2401 tgatcgagaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     2461 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     2521 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     2581 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     2641 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     2701 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     2761 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     2821 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     2881 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttaccatg
     2941 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     3001 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     3061 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     3121 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     3181 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     3241 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     3301 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     3361 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     3421 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     3481 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaatc
     3541 gatagatcct aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     3601 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     3661 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     3721 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     3781 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     3841 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     3901 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     3961 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4021 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4081 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     4141 tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     4201 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     4261 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4321 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     4381 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     4441 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     4501 atatcagaga gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt cttgaagacg
     4561 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     4621 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4681 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4741 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     4801 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     4861 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     4921 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     4981 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5041 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5101 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5161 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5221 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5281 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     5341 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     5401 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     5461 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5521 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5581 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     5641 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5701 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     5761 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     5821 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5881 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     5941 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6001 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6061 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6121 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6181 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6241 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6301 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6361 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6421 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6481 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6541 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     6601 tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc
     6661 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     6721 tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     6781 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     6841 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     6901 catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6961 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
     7021 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
     7081 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     7141 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
     7201 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     7261 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     7321 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     7381 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     7441 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     7501 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     7561 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     7621 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7681 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7741 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     7801 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     7861 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     7921 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     7981 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     8041 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     8101 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     8161 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     8221 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     8281 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     8341 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     8401 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     8461 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     8521 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     8581 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     8641 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     8701 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     8761 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     8821 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     8881 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     8941 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     9001 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     9061 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     9121 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     9181 gtatcgccga attcacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     9241 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     9301 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     9361 gatccagttt ggactagtgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     9421 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     9481 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     9541 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     9601 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     9661 catctacgta ttagtcatcg ctattaccat g
//
