LOCUS       sequence-230451-        8608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express ZFP64 without transcription activation domain(TAD).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8608)
  AUTHORS   Lu B, Klingbeil O, Tarumoto Y, Somerville TDD, Huang YH, Wei Y, Wai 
            DC, Low JKK, Milazzo JP, Wu XS, Cao Z, Yan X, Demerdash OE, Huang G,
            Mackay JP, Kinney JB, Shi J, Vakoc CR
  TITLE     A Transcription Factor Addiction in Leukemia Imposed by the MLL 
            Promoter Sequence.
  JOURNAL   Cancer Cell. 2018 Dec 10;34(6):970-981.e8. doi: 
            10.1016/j.ccell.2018.10.015. Epub 2018 Nov 29.
  PUBMED    30503706
REFERENCE   2  (bases 1 to 8608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.10.015"; journalName: "Cancer Cell"; date: 
            "2018-12-10- 10"; volume: "34"; issue: "6"; pages: "970-981.e8"
COMMENT     Sequence Label: LentiV_Neo_ZFP64_Delta_TAD
FEATURES             Location/Qualifiers
     source          1..8608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    266..499
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             684..728
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    912..1029
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1098..1309
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      1329..1338
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1338..1403
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2769..2825
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2844..3638
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2898..2917)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3508..3527
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    3654..4242
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3707..3727)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4125..4136)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4314..4547
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4625..4746
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4662..4681)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4716..4735
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4786..4921
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4941..4960)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4942..4960)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4970..4987)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4970..4986)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4979..5001)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5128..5583
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5215..5234)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5425..5446
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5609..5713
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5714..5782,5783..6574)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5932..5951)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6745..7333
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7234..7253
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7487..7504
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7621..7642
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7657..7662,7663..7680,7681..7687)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7695..7711
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7700..7722
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7719..7735
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7719..7735
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7754..7774
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7756..7774
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7802..8028
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8029..8209
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8256..8381
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
       61 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
      121 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
      181 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
      241 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
      301 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
      361 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
      421 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
      481 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
      541 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
      601 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
      661 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
      721 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
      781 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
      841 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
      901 ccagaccaca attttaaaag aaaagggggg attgggaggt acagtgcagg ggaaagaata
      961 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     1021 caaaattttc gggtttatta cagggacagc agagatccac tttggcgccg gctcgagtgg
     1081 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     1141 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     1201 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     1261 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg tgtcgtgacg
     1321 cgggatccgc caccatggat tacaaagacc atgacggtga ttataaagat catgatatcg
     1381 attacaagga tgacgatgac aagatgaacg cgagcagcga gggcgagagc ttcgcgggct
     1441 cggtgcaaat tccaggtggc acaacggtgc tggtggagct gactcccgac atccacattt
     1501 gtggaatttg taaacagcag tttaacaacc tggatgcctt tgtagctcac aagcaaagtg
     1561 gctgccagct gacaggcaca tccgcagcag cccccagcac ggtccagttt gtatcggagg
     1621 aaacagtgcc tgccacccag actcagacca ccaccagaac catcacctcg gagacccaga
     1681 caatcacagt ttcagctcca gaatttgttt ttgaacatgg ctatcaaact tacctgccca
     1741 cggaaagtaa tgaaaaccag acagccactg tcatctctct ccctgccaag tcacgcacca
     1801 aaaagcccac aacaccacct gctcagaaaa ggcttaactg ttgctatcca ggttgccaat
     1861 tcaagactgc ttatggcatg aaggacatgg agcggcattt aaaaattcac acgggagaca
     1921 aaccccataa gtgtgaagtc tgtggcaagt gctttagccg gaaagacaag ctgaaaactc
     1981 acatgcggtg ccacacgggc gtgaagccct acaagtgtaa gacgtgtgac tacgccgctg
     2041 ccgacagcag cagcctcaac aagcacctga ggatccactc ggacgagcgg cccttcaaat
     2101 gccagatctg cccctacgcc agccgcaact ccagccagct cactgtccac ctgcgatccc
     2161 acacggggga cgcccccttc cagtgctggc tctgtagcgc caagttcaaa atcagctcgg
     2221 acttgaaaag gcacatgcgg gtgcactcgg gggagaagcc tttcaagtgc gagttctgca
     2281 atgtccgctg caccatgaag gggaacctca agtcgcacat ccgtatcaag cacagcggga
     2341 ataacttcaa gtgtcctcat tgcgacttcc tgggtgacag caaagccacc ctccggaagc
     2401 acagccgcgt gcaccagtcg gagcatcctg agaagtgctc ggaatgcagc tactcctgct
     2461 ccagcaaggc cgccctgcgc atccacgagc gtatccactg caccgaccgc cctttcaagt
     2521 gcaactactg cagcttcgac accaaacagc ccagcaacct gagcaagcac atgaagaagt
     2581 tccacgggga catggttaag actgaggctc tagagaggaa ggacaccggc aggcagagca
     2641 gccggcaggt ggccaagctg gatgccaaga agagtttcca ctgcgatata tgcgatgcct
     2701 ccttcatgcg ggaggactcg ctccgcagcc acaagagaca gcacagtgag tacagtgagg
     2761 gcagcggcgc caccaacttc agcctgctga agcaggccgg cgacgtggag gagaaccccg
     2821 gccccatgac cgagtacaag cccatgattg aacaagatgg attgcacgca ggttctccgg
     2881 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     2941 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     3001 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     3061 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     3121 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     3181 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     3241 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     3301 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     3361 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     3421 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     3481 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     3541 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     3601 gcatcgcctt ctatcgcctt cttgacgagt tcttctgaac gcgttaagtc gacaatcaac
     3661 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3721 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3781 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     3841 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     3901 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     3961 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     4021 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     4081 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     4141 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     4201 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat
     4261 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     4321 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     4381 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     4441 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     4501 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag
     4561 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     4621 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     4681 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     4741 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     4801 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4861 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4921 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     4981 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     5041 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     5101 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     5161 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5221 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5281 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5341 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5401 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5461 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5521 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     5581 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5641 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5701 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5761 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5821 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5881 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5941 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     6001 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6061 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6121 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6181 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6241 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6301 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6361 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6421 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6481 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6541 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6601 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6661 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6721 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6781 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6841 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     6901 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6961 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7021 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7081 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7141 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7201 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7261 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7321 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7381 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     7441 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     7501 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     7561 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     7621 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     7681 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     7741 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
     7801 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     7861 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     7921 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
     7981 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
     8041 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     8101 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     8161 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     8221 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     8281 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     8341 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     8401 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     8461 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     8521 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     8581 cagaagaact tagatcatta tataatac
//
