LOCUS       sequence-230452-        6264 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express MLL2/KMT2B promoter for luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Lu B, Klingbeil O, Tarumoto Y, Somerville TDD, Huang YH, Wei Y, Wai 
            DC, Low JKK, Milazzo JP, Wu XS, Cao Z, Yan X, Demerdash OE, Huang G,
            Mackay JP, Kinney JB, Shi J, Vakoc CR
  TITLE     A Transcription Factor Addiction in Leukemia Imposed by the MLL 
            Promoter Sequence.
  JOURNAL   Cancer Cell. 2018 Dec 10;34(6):970-981.e8. doi: 
            10.1016/j.ccell.2018.10.015. Epub 2018 Nov 29.
  PUBMED    30503706
REFERENCE   2  (bases 1 to 6264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.10.015"; journalName: "Cancer Cell"; date: 
            "2018-12-10- 10"; volume: "34"; issue: "6"; pages: "970-981.e8"
COMMENT     Sequence Label: pGL421_MLL2_promoter
FEATURES             Location/Qualifiers
     source          1..6264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1411..1530
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    1582..1703
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1593..1612)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1647..1666
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1897..2254
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1897..2088
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1920..1940)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2105..2240
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2167..2186
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2285..2884
                     /codon_start=1
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    2909..2957
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(3113..3130)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3284..3872)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3364..3383)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4072..4863,4864..4932))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5037..5085
                     /note="synthetic polyadenylation signal"
     misc_feature    5099..5190
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5139..5158
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      6016..6025
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6022..6264,1..1407)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat cggtgtggct
       61 gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat gggcatcagc
      121 cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa cgtgcaaaag
      181 aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta ccagggcttc
      241 caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga gtacgacttc
      301 gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag tagtggcagt
      361 accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg attcagtcat
      421 gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct cagcgtggtg
      481 ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg cggctttcgg
      541 gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca agactataag
      601 attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag cactctcatc
      661 gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc gctcagcaag
      721 gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca gggctacggc
      781 ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa gcctggcgca
      841 gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac cggtaagaca
      901 ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat gagcggctac
      961 gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct gcacagcggc
     1021 gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct gaagagcctg
     1081 atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct gctgcaacac
     1141 cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc
     1201 gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat cgtggactat
     1261 gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt cgtggacgag
     1321 gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat tctcattaag
     1381 gccaagaagg gcggcaagat cgccgtgaat tctcacggct tccctcccga ggtggaggag
     1441 caggccgccg gcaccctgcc catgagctgc gcccaggaga gcggcatgga tagacaccct
     1501 gctgcttgcg ccagcgccag gatcaacgtc taaggccgcg actctagagt cggggcggcc
     1561 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     1621 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     1681 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     1741 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg
     1801 ataaggatcc gtttgcgtat tgggcgctct tccgctgatc tgcgcagcac catggcctga
     1861 aataacctct gaaagaggaa cttggttagc taccttctga ggcggaaaga accagctgtg
     1921 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     1981 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     2041 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     2101 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     2161 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     2221 aggaggcttt tttggaggcc taggcttttg caaaaagctc gattcttctg acactagcgc
     2281 caccatgacc gagtacaagc ctaccgtgcg cctggccact cgcgatgatg tgccccgcgc
     2341 cgtccgcact ctggccgccg ctttcgccga ctaccccgct acccggcaca ccgtggaccc
     2401 cgaccggcac atcgagcgtg tgacagagtt gcaggagctg ttcctgaccc gcgtcgggct
     2461 ggacatcggc aaggtgtggg tagccgacga cggcgcggcc gtggccgtgt ggactacccc
     2521 cgagagcgtt gaggccggcg ccgtgttcgc cgagatcggc ccccgaatgg ccgagctgag
     2581 cggcagccgc ctggccgccc agcagcaaat ggagggcctg cttgcccccc atcgtcccaa
     2641 ggagcctgcc tggtttctgg ccactgtagg agtgagcccc gaccaccagg gcaagggctt
     2701 gggcagcgcc gtcgtgttgc ccggcgtaga ggccgccgaa cgcgccggtg tgcccgcctt
     2761 tctcgaaaca agcgcaccaa gaaaccttcc attctacgag cgcctgggct tcaccgtgac
     2821 cgccgatgtc gaggtgcccg agggacctag gacctggtgt atgacacgaa aacctggcgc
     2881 ctaatgatct agaaccggtc atggccgcaa taaaatatct ttattttcat tacatctgtg
     2941 tgttggtttt ttgtgtgttc gaactagatg ctgtcgaccg atgcccttga gagccttcaa
     3001 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt
     3061 cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct tcctcgctca
     3121 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3181 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     3241 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3301 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3361 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3421 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3481 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3541 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3601 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3661 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     3721 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     3781 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     3841 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     3901 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     3961 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4021 atgagtaaac ttggtctgac agcggccgca aatgctaaac cactgcagtg gttaccagtg
     4081 cttgatcagt gaggcaccga tctcagcgat ctgcctattt cgttcgtcca tagtggcctg
     4141 actccccgtc gtgtagatca ctacgattcg tgagggctta ccatcaggcc ccagcgcagc
     4201 aatgatgccg cgagagccgc gttcaccggc ccccgatttg tcagcaatga accagccagc
     4261 agggagggcc gagcgaagaa gtggtcctgc tactttgtcc gcctccatcc agtctatgag
     4321 ctgctgtcgt gatgctagag taagaagttc gccagtgagt agtttccgaa gagttgtggc
     4381 cattgctact ggcatcgtgg tatcacgctc gtcgttcggt atggcttcgt tcaactctgg
     4441 ttcccagcgg tcaagccggg tcacatgatc acccatatta tgaagaaatg cagtcagctc
     4501 cttagggcct ccgatcgttg tcagaagtaa gttggccgcg gtgttgtcgc tcatggtaat
     4561 ggcagcacta cacaattctc ttaccgtcat gccatccgta agatgctttt ccgtgaccgg
     4621 cgagtactca accaagtcgt tttgtgagta gtgtatacgg cgaccaagct gctcttgccc
     4681 ggcgtctata cgggacaaca ccgcgccaca tagcagtact ttgaaagtgc tcatcatcgg
     4741 gaatcgttct tcggggcgga aagactcaag gatcttgccg ctattgagat ccagttcgat
     4801 atagcccact cttgcaccca gttgatcttc agcatctttt actttcacca gcgtttcggg
     4861 gtgtgcaaaa acaggcaagc aaaatgccgc aaagaaggga atgagtgcga cacgaaaatg
     4921 ttggatgctc atactcgtcc tttttcaata ttattgaagc atttatcagg gttactagta
     4981 cgtctctcaa ggataagtaa gtaatattaa ggtacgggag gtattggaca ggccgcaata
     5041 aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa
     5101 catacgctct ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca
     5161 gtgcaagtgc aggtgccaga acatttctct ggcctaactg gccggtacct gagctcgcta
     5221 gcctcgagga tatcccttcc actgcaatcc acggcgcgcg gagtctgatc tgggaacgct
     5281 cggcccggga gagagaggaa cgcgtgctcc gaccctgctt tcggccaccc gcctcctctc
     5341 cgggagcaca cggaccccga gaccgcggcc tctccggcgt cttccctcca caccgtgcca
     5401 agccgccgcc aggtcgcgcc tggaacggag actgactatg ctttccaccg ctccttctcc
     5461 agcccacgcc tcctgcagcc gcgaactgca tttcccaggg cctcgggggt cccggggccc
     5521 gagcccgcgg caccctggga gtggtagtcc acgcgggccg ccggcctagc caagaacaat
     5581 gggaggagcc tggggctagg accacacctc ccggagaccc ggcaacccgc tttccgggtc
     5641 ctggccggga accccgccgt cccggccctc gcgccctggc tgccggcgga agaagcgagg
     5701 cgtccggact acatttcccg gaggcccccg cggttcgccc ctctgcggcc gcgacgcggg
     5761 ggtctgtgct ccccaggtgc ggtccgaggg cgccggccgg ccgcggactc cctctcccgt
     5821 ggtgccctgc gcgtggccgg cccggccccc ggtctcccgc ggcggcgctg gttgttgtcg
     5881 tgcgccgcgg ccgccccgcc ccgcccccgc tctctcccct ccccccggcc ctgcgcacgg
     5941 gccgcccctc cccccgcctc cccggcccct ctcacggtgc caagataagc ttggcaatcc
     6001 ggtactgttg gtaaagccac catggaagat gccaaaaaca ttaagaaggg cccagcgcca
     6061 ttctacccac tcgaagacgg gaccgccggc gagcagctgc acaaagccat gaagcgctac
     6121 gccctggtgc ccggcaccat cgcctttacc gacgcacata tcgaggtgga cattacctac
     6181 gccgagtact tcgagatgag cgttcggctg gcagaagcta tgaagcgcta tgggctgaat
     6241 acaaaccatc ggatcgtggt gtgc
//
