LOCUS       sequence-230454-       13742 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The human desmoplakin II is C-terminally tagged with YPet(short) to 
            serve as wild type control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13742)
  AUTHORS   Price AJ, Cost AL, Ungewiss H, Waschke J, Dunn AR, Grashoff C
  TITLE     Mechanical loading of desmosomes depends on the magnitude and 
            orientation of external stress.
  JOURNAL   Nat Commun. 2018 Dec 11;9(1):5284. doi: 10.1038/s41467-018-07523-0.
  PUBMED    30538252
REFERENCE   2  (bases 1 to 13742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-11"; volume: "9(1):5284. doi"; pages: " 
            10.1038/s41467-018-07523-0"
COMMENT     Sequence Label: Desmoplakin II-YPet(short)
FEATURES             Location/Qualifiers
     source          1..13742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             414..589
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    652..851
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1052..1468
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1398..1420
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1436..1452
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1565..2164
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1565..1584)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2061..2081
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     enhancer        2339..2642
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2643..2846
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2797..2817
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(10055..10074)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     LTR             10481..11074
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(11177..11199)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    11327..11360
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    11420..11453
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     11473..11492
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11573..11590)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11744..12332)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11824..11843)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12503..13294,13295..13363))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13126..13145
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13364..13468)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13536..13554
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
     1441 tccagccctc actccttctc taggcgccgg aatgatcccc cgggctgcag gtcggccgcc
     1501 acgaccggtg ccgccaccat cccctgaccc acgcccctga cccctcacaa ggagacgacc
     1561 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     1621 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     1681 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     1741 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     1801 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     1861 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     1921 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     1981 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     2041 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     2101 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     2161 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatggctc
     2221 cgaccgaagc cacccggggc ggccccgccg accccgcacc cgcccccgag gcccaccgac
     2281 tctagtaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     2341 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     2401 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     2461 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     2521 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     2581 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     2641 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     2701 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     2761 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     2821 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
     2881 ggactcagat ctgggcccat aaagcttata cgaattcacc atgagctgca acggaggctc
     2941 ccacccgcgg atcaacactc tgggccgcat gatccgcgcc gagtctggcc cggacctgcg
     3001 ctacgaggtg accagcggcg gcgggggcac cagcaggatg tactattctc ggcgcggcgt
     3061 gatcaccgac cagaactcgg acggctactg tcaaaccggc acgatgtcca ggcaccagaa
     3121 ccagaacacc atccaggagc tgctgcagaa ctgctccgac tgcttgatgc gagcagagct
     3181 catcgtgcag cctgaattga agtatggaga tggaatacaa ctgactcgga gtcgagaatt
     3241 ggatgagtgt tttgcccagg ccaatgacca aatggaaatc ctcgacagct tgatcagaga
     3301 gatgcggcag atgggccagc cctgtgatgc ttaccagaaa aggcttcttc agctccaaga
     3361 gcaaatgcga gccctttata aagccatcag tgtcccacga gtccgcaggg ccagctccaa
     3421 gggtggtgga ggctacactt gtcagagtgg ctctggctgg gatgagttca ccaaacatgt
     3481 caccagtgaa tgtttggggt ggatgaggca gcaaagggcg gagatggaca tggtggcctg
     3541 gggtgtggac ctggcctcag tggagcagca cattaacagc caccggggca tccacaactc
     3601 catcggcgac tatcgctggc agctggacaa aatcaaagcc gacctgcgcg agaaatctgc
     3661 gatctaccag ttggaggagg agtatgaaaa cctgctgaaa gcgtcctttg agaggatgga
     3721 tcacctgcga cagctgcaga acatcattca ggccacgtcc agggagatca tgtggatcaa
     3781 tgactgcgag gaggaggagc tgctgtacga ctggagcgac aagaacacca acatcgctca
     3841 gaaacaggag gccttctcca tacgcatgag tcaactggaa gttaaagaaa aagagctcaa
     3901 taagctgaaa caagaaagtg accaacttgt cctcaatcag catccagctt cagacaaaat
     3961 tgaggcctat atggacactc tgcagacgca gtggagttgg attcttcaga tcaccaagtg
     4021 cattgatgtt catctgaaag aaaatgctgc ctactttcag ttttttgaag aggcgcagtc
     4081 tactgaagca tacctgaagg ggctccagga ctccatcagg aagaagtacc cctgcgacaa
     4141 gaacatgccc ctgcagcacc tgctggaaca gatcaaggag ctggagaaag aacgagagaa
     4201 aatccttgaa tacaagcgtc aggtgcagaa cttggtaaac aagtctaaga agattgtaca
     4261 gctgaagcct cgtaacccag actacagaag caataaaccc attattctca gagctctctg
     4321 tgactacaaa caagatcaga aaatcgtgca taagggggat gagtgtatcc tgaaggacaa
     4381 caacgagcgc agcaagtggt acgtgacggg cccgggaggc gttgacatgc ttgttccctc
     4441 tgtggggctg atcatccctc ctccgaaccc actggccgtg gacctctctt gcaagattga
     4501 gcagtactac gaagccatct tggctctgtg gaaccagctc tacatcaaca tgaagagcct
     4561 ggtgtcctgg cactactgca tgattgacat agagaagatc agggccatga caatcgccaa
     4621 gctgaaaaca atgcggcagg aagattacat gaagacgata gccgaccttg agttacatta
     4681 ccaagagttc atcagaaata gccaaggctc agagatgttt ggagatgatg acaagcggaa
     4741 aatacagtct cagttcaccg atgcccagaa gcattaccag accctggtca ttcagctccc
     4801 tggctatccc cagcaccaga cagtgaccac aactgaaatc actcatcatg gaacctgcca
     4861 agatgtcaac cataataaag taattgaaac caacagagaa aatgacaagc aagaaacatg
     4921 gatgctgatg gagctgcaga agattcgcag gcagatagag cactgcgagg gcaggatgac
     4981 tctcaaaaac ctccctctag cagaccaggg gtcttctcac cacatcacag tgaaaattaa
     5041 cgagcttaag agtgtgcaga atgattcaca agcaattgct gaggttctca accagcttaa
     5101 agatatgctt gccaacttca gaggttctga aaagtactgc tatttacaga atgaagtatt
     5161 tggactattt cagaaactgg aaaatatcaa tggtgttaca gatggctact taaatagctt
     5221 atgcacagta agggcactgc tccaggctat tctccaaaca gaagacatgt taaaggttta
     5281 tgaagccagg ctcactgagg aggaaactgt ctgcctggac ctggataaag tggaagctta
     5341 ccgctgtgga ctgaagaaaa taaaaaatga cttgaacttg aagaagtcgt tgttggccac
     5401 tatgaagaca gaactacaga aagcccagca gatccactct cagacttcac agcagtatcc
     5461 actttatgat ctggacttgg gcaagttcgg tgaaaaagtc acacagctga cagaccgctg
     5521 gcaaaggata gataaacaga tcgactttag attatgggac ctggagaaac aaatcaagca
     5581 attgaggaat tatcgtgata actatcaggc tttctgcaag tggctctatg atgctaaacg
     5641 ccgccaggat tccttagaat ccatgaaatt tggagattcc aacacagtca tgcggttttt
     5701 gaatgagcag aagaacttgc acagtgaaat atctggcaaa cgagacaaat cagaggaagt
     5761 acaaaaaatt gctgaacttt gcgccaattc aattaaggat tatgagctcc agctggcctc
     5821 atacacctca ggactggaaa ctctgctgaa catacctatc aagaggacca tgattcagtc
     5881 cccttctggg gtgattctgc aagaggctgc agatgttcat gctcggtaca ttgaactact
     5941 tacaagatct ggagactatt acaggttctt aagtgagatg ctgaagagtt tggaagatct
     6001 gaagctgaaa aataccaaga tcgaagtttt ggaagaggag ctcagactgg cccgagatgc
     6061 caactcggaa aactgtaata agaacaaatt cctggatcag aacctgcaga aataccaggc
     6121 agagtgttcc cagttcaaag cgaagcttgc gagcctggag gagctgaaga gacaggctga
     6181 gctggatggg aagtcggcta agcaaaatct agacaagtgc tacggccaaa taaaagaact
     6241 caatgagaag atcacccgac tgacttatga gattgaagat gaaaagagaa gaagaaaatc
     6301 tgtggaagac agatttgacc aacagaagaa tgactatgac caactgcaga aagcaaggca
     6361 atgtgaaaag gagaaccttg gttggcagaa attagagtct gagaaagcca tcaaggagaa
     6421 ggagtacgag attgaaaggt tgagggttct actgcaggaa gaaggcaccc ggaagagaga
     6481 atatgaaaat gagctggcaa aggcatctaa taggattcag gaatcaaaga atcagtgtac
     6541 tcaggtggta caggaaagag agagccttct ggtgaaaatc aaagtcctgg agcaagacaa
     6601 ggcaaggctg cagaggctgg aggatgagct gaatcgtgca aaatcaactc tagaggcaga
     6661 aaccagggtg aaacagcgcc tggagtgtga gaaacagcaa attcagaatg acctgaatca
     6721 gtggaagact caatattccc gcaaggagga ggctattagg aagatagaat cggaaagaga
     6781 aaagagtgag agagagaaga acagtcttag gagtgagatc gaaagactcc aagcagagat
     6841 caagagaatt gaagagaggt gcaggcgtaa gctggaggat tctaccaggg agacacagtc
     6901 acagttagaa acagaacgct cccgatatca gagggagatt gataaactca gacagcgccc
     6961 atatgggtcc catcgagaga cccagactga gtgtgagtgg accgttgaca cctccaagct
     7021 ggtgtttgat gggctgagga agaaggtgac agcaatgcag ctctatgagt gtcagctgat
     7081 cgacaaaaca accttggaca aactattgaa ggggaagaag tcagtggaag aagttgcttc
     7141 tgaaatccag ccattccttc ggggtgcagg atctatcgct ggagcatctg cttctcctaa
     7201 ggaaaaatac tctttggtag aggccaagag aaagaaatta atcagcccag aatccacagt
     7261 catgcttctg gaggcccagg cagctacagg tggtataatt gatccccatc ggaatgagaa
     7321 gctgactgtg gacagtgcca tagctcggga cctcattgac ttcgatgacc gtcagcagat
     7381 atatgcagca gaaaaagcta tcactggttt tgatgatcca ttttcaggca agacagtatc
     7441 tgtttcagaa gccatcaaga aaaatttgat tgatagagaa accggaatgc gcctgctgga
     7501 agcccagatt gcttcagggg gtgtagtaga ccctgtgaac agtgtctttt tgccaaaaga
     7561 tgtcgccttg gcccgggggc tgattgatag agatttgtat cgttccctga atgatccccg
     7621 agatagtcag aaaaactttg tggatccagt caccaaaaag aaggtcagtt acgtgcagct
     7681 gaaggaacgg tgcagaatcg aaccacatac tggtctgctc ttgctttcag tacagaagag
     7741 aagcatgtcc ttccaaggaa tcagacaacc tgtgaccgtc actgagctag tagattctgg
     7801 tatattgaga ccgtccactg tcaatgaact ggaatctggt cagatttctt atgacgaggt
     7861 tggtgagaga attaaggact tcctccaggg ttcaagctgc atagcaggca tatacaatga
     7921 gaccacaaaa cagaagcttg gcatttatga ggccatgaaa attggcttag tccgacctgg
     7981 tactgctctg gagttgctgg aagcccaagc agctactggc tttatagtgg atcctgttag
     8041 caacttgagg ttaccagtgg aggaagccta caagagaggt ctggtgggca ttgagttcaa
     8101 agagaagctc ctgtctgcag aacgagctgt cactgggtat aatgatcctg aaacaggaaa
     8161 catcatctct ttgttccaag ccatgaataa ggaactcatc gaaaagggcc acggtattcg
     8221 cttattagaa gcacagatcg caaccggggg gatcattgac ccaaaggaga gccatcgttt
     8281 accagttgac atagcatata agaggggcta tttcaatgag gaactcagtg agattctctc
     8341 agatccaagt gatgatacca aaggattttt tgaccccaac actgaagaaa atcttaccta
     8401 tctgcaacta aaagaaagat gcattaagga tgaggaaaca gggctctgtc ttctgcctct
     8461 gaaagaaaag aagaaacagg tgcagacatc acaaaagaat accctcagga agcgtagagt
     8521 ggtcatagtt gacccagaaa ccaataaaga aatgtctgtt caggaggcct acaagaaggg
     8581 cctaattgat tatgaaacct tcaaagaact gtgtgagcag gaatgtgaat gggaagaaat
     8641 aaccatcacg ggatcagatg gctccaccag ggtggtcctg gtagatagaa agacaggcag
     8701 tcagtatgat attcaagatg ctattgacaa gggccttgtt gacaggaagt tctttgatca
     8761 gtaccgatcc ggcagcctca gcctcactca atttgctgac atgatctcct tgaaaaatgg
     8821 tgtcggcacc agcagcagca tgggcagtgg tgtcagcgat gatgttttta gcagctcccg
     8881 acatgaatca gtaagtaaga tttccaccat atccagcgtc aggaatttaa ccataaggag
     8941 cagctctttt tcagacaccc tggaagaatc gagccccatt gcagccatct ttgacacaga
     9001 aaacctggag aaaatctcca ttacagaagg tatagagcgg ggcatcgttg acagcatcac
     9061 gggtcagagg cttctggagg ctcaggcctg cacaggtggc atcatccacc caaccacggg
     9121 ccagaagctg tcacttcagg acgcagtctc ccagggtgtg attgaccaag acatggccac
     9181 caggctgaag cctgctcaga aagccttcat aggcttcgag ggtgtgaagg gaaagaagaa
     9241 gatgtcagca gcagaggcag tgaaagaaaa atggctcccg tatgaggctg gccagcgctt
     9301 cctggagttc cagtacctca cgggaggtct tgttgacccg gaagtgcatg ggaggataag
     9361 caccgaagaa gccatccgga aggggttcat agatggccgc gccgcacaga ggctgcaaga
     9421 caccagcagc tatgccaaaa tcctgacctg ccccaaaacc aaattaaaaa tatcctataa
     9481 ggatgccata aatcgctcca tggtagaaga tatcactggg ctgcgccttc tggaagccgc
     9541 ctccgtgtcg tccaagggct tacccagccc ttacaacatg tcttcggctc cggggtcccg
     9601 ctccggctcc cgctcgggat ctcgctccgg atctcgctcc gggtcccgca gtgggtcccg
     9661 gagaggaagc tttgacgcca caggcaattc ttcctactct tattcctact catttagcag
     9721 tagttctatt gggcacgggg cggccgcagg agtgagcaaa ggcgaagagc tgttcaccgg
     9781 cgtggtgccc atcctggtgg agctggacgg cgacgtgaac ggccacaagt tcagcgtgag
     9841 cggcgagggc gagggcgacg ccacctacgg caagctgacc ctgaagctgc tgtgcaccac
     9901 cggcaagctg cccgtgccct ggcccaccct ggtgaccacc ctgggctacg gcgtgcagtg
     9961 cttcgcccgg taccccgacc acatgaagca gcacgacttc ttcaagagcg ccatgcccga
    10021 gggctacgtg caggagcgga ccatcttctt caaggacgac ggcaactaca agacccgggc
    10081 cgaggtgaag ttcgagggcg acaccctggt gaaccggatc gagctgaagg gcatcgactt
    10141 caaggaggac ggcaacatcc tgggccacaa gctggagtac aactacaaca gccacaacgt
    10201 gtacatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccggcacaa
    10261 catcgaggac ggcggcgtgc agctggccga ccactaccag cagaacaccc ccatcggcga
    10321 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagagcgccc tgttcaagga
    10381 ccccaacgag aagcgggacc acatggtgct gctggagttc ctgaccgccg cctaaatcga
    10441 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
    10501 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga
    10561 gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca
    10621 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg
    10681 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
    10741 cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc
    10801 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg
    10861 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc
    10921 tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa
    10981 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
    11041 gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac
    11101 ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggctgcctc gcgcgtttcg
    11161 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
    11221 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
    11281 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtactgataa cttcgtataa
    11341 tgtatgctat acgaagttat taggtctgaa gaggagttta cgtccagcca agctagggcc
    11401 gcgatccgga acccttaata taacttcgta taatgtatgc tatacgaagt tatcagtact
    11461 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
    11521 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
    11581 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    11641 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    11701 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    11761 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    11821 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    11881 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    11941 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    12001 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    12061 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    12121 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    12181 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    12241 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    12301 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    12361 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    12421 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    12481 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    12541 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    12601 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    12661 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    12721 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    12781 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct
    12841 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    12901 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    12961 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    13021 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    13081 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
    13141 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    13201 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    13261 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    13321 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    13381 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    13441 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
    13501 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
    13561 gtcttcaaga attgctagca attgctagca attgctagca attcatacca gatcaccgaa
    13621 aactgtcctc caaatgtgtc cccctcacac tcccaaattc gcgggcttct gcctcttaga
    13681 ccactctacc ctattcccca cactcaccgg agccaaagcc gcggcccttc cgtttctttg
    13741 ct
//
