LOCUS       sequence-230460-       11856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The donor (YPet(short)) only control for the no force control of the
            F40-based human desmoplakin II tension sensor provides the donor 
            only lifetime used in lifetime measurements to determine FRET..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11856)
  AUTHORS   Price AJ, Cost AL, Ungewiss H, Waschke J, Dunn AR, Grashoff C
  TITLE     Mechanical loading of desmosomes depends on the magnitude and 
            orientation of external stress.
  JOURNAL   Nat Commun. 2018 Dec 11;9(1):5284. doi: 10.1038/s41467-018-07523-0.
  PUBMED    30538252
REFERENCE   2  (bases 1 to 11856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-11"; volume: "9(1):5284. doi"; pages: " 
            10.1038/s41467-018-07523-0"
COMMENT     Sequence Label: Desmoplakin II donor only control (F40-based n...
FEATURES             Location/Qualifiers
     source          1..11856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             414..589
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    652..851
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1052..1468
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1398..1420
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1436..1452
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1565..2164
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1565..1584)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2061..2081
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     enhancer        2339..2642
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2643..2846
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2797..2817
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(7289..7308)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             7799..8509
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMLGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7944..7962)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8212..8231
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             8595..9188
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(9291..9313)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    9441..9474
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    9534..9567
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     9587..9606
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9687..9704)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9858..10446)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9938..9957)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10617..11408,11409..11477))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11240..11259
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11478..11582)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11650..11668
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
     1441 tccagccctc actccttctc taggcgccgg aatgatcccc cgggctgcag gtcggccgcc
     1501 acgaccggtg ccgccaccat cccctgaccc acgcccctga cccctcacaa ggagacgacc
     1561 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     1621 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     1681 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     1741 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     1801 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     1861 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     1921 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     1981 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     2041 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     2101 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     2161 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatggctc
     2221 cgaccgaagc cacccggggc ggccccgccg accccgcacc cgcccccgag gcccaccgac
     2281 tctagtaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     2341 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     2401 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     2461 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     2521 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     2581 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     2641 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     2701 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     2761 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     2821 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
     2881 ggactcagat ctgggcccat aaagcttata cgaattcatg agctgcaacg gaggctccca
     2941 cccgcggatc aacactctgg gccgcatgat ccgcgccgag tctggcccgg acctgcgcta
     3001 cgaggtgacc agcggcggcg ggggcaccag caggatgtac tattctcggc gcggcgtgat
     3061 caccgaccag aactcggacg gctactgtca aaccggcacg atgtccaggc accagaacca
     3121 gaacaccatc caggagctgc tgcagaactg ctccgactgc ttgatgcgag cagagctcat
     3181 cgtgcagcct gaattgaagt atggagatgg aatacaactg actcggagtc gagaattgga
     3241 tgagtgtttt gcccaggcca atgaccaaat ggaaatcctc gacagcttga tcagagagat
     3301 gcggcagatg ggccagccct gtgatgctta ccagaaaagg cttcttcagc tccaagagca
     3361 aatgcgagcc ctttataaag ccatcagtgt cccacgagtc cgcagggcca gctccaaggg
     3421 tggtggaggc tacacttgtc agagtggctc tggctgggat gagttcacca aacatgtcac
     3481 cagtgaatgt ttggggtgga tgaggcagca aagggcggag atggacatgg tggcctgggg
     3541 tgtggacctg gcctcagtgg agcagcacat taacagccac cggggcatcc acaactccat
     3601 cggcgactat cgctggcagc tggacaaaat caaagccgac ctgcgcgaga aatctgcgat
     3661 ctaccagttg gaggaggagt atgaaaacct gctgaaagcg tcctttgaga ggatggatca
     3721 cctgcgacag ctgcagaaca tcattcaggc cacgtccagg gagatcatgt ggatcaatga
     3781 ctgcgaggag gaggagctgc tgtacgactg gagcgacaag aacaccaaca tcgctcagaa
     3841 acaggaggcc ttctccatac gcatgagtca actggaagtt aaagaaaaag agctcaataa
     3901 gctgaaacaa gaaagtgacc aacttgtcct caatcagcat ccagcttcag acaaaattga
     3961 ggcctatatg gacactctgc agacgcagtg gagttggatt cttcagatca ccaagtgcat
     4021 tgatgttcat ctgaaagaaa atgctgccta ctttcagttt tttgaagagg cgcagtctac
     4081 tgaagcatac ctgaaggggc tccaggactc catcaggaag aagtacccct gcgacaagaa
     4141 catgcccctg cagcacctgc tggaacagat caaggagctg gagaaagaac gagagaaaat
     4201 ccttgaatac aagcgtcagg tgcagaactt ggtaaacaag tctaagaaga ttgtacagct
     4261 gaagcctcgt aacccagact acagaagcaa taaacccatt attctcagag ctctctgtga
     4321 ctacaaacaa gatcagaaaa tcgtgcataa gggggatgag tgtatcctga aggacaacaa
     4381 cgagcgcagc aagtggtacg tgacgggccc gggaggcgtt gacatgcttg ttccctctgt
     4441 ggggctgatc atccctcctc cgaacccact ggccgtggac ctctcttgca agattgagca
     4501 gtactacgaa gccatcttgg ctctgtggaa ccagctctac atcaacatga agagcctggt
     4561 gtcctggcac tactgcatga ttgacataga gaagatcagg gccatgacaa tcgccaagct
     4621 gaaaacaatg cggcaggaag attacatgaa gacgatagcc gaccttgagt tacattacca
     4681 agagttcatc agaaatagcc aaggctcaga gatgtttgga gatgatgaca agcggaaaat
     4741 acagtctcag ttcaccgatg cccagaagca ttaccagacc ctggtcattc agctccctgg
     4801 ctatccccag caccagacag tgaccacaac tgaaatcact catcatggaa cctgccaaga
     4861 tgtcaaccat aataaagtaa ttgaaaccaa cagagaaaat gacaagcaag aaacatggat
     4921 gctgatggag ctgcagaaga ttcgcaggca gatagagcac tgcgagggca ggatgactct
     4981 caaaaacctc cctctagcag accaggggtc ttctcaccac atcacagtga aaattaacga
     5041 gcttaagagt gtgcagaatg attcacaagc aattgctgag gttctcaacc agcttaaaga
     5101 tatgcttgcc aacttcagag gttctgaaaa gtactgctat ttacagaatg aagtatttgg
     5161 actatttcag aaactggaaa atatcaatgg tgttacagat ggctacttaa atagcttatg
     5221 cacagtaagg gcactgctcc aggctattct ccaaacagaa gacatgttaa aggtttatga
     5281 agccaggctc actgaggagg aaactgtctg cctggacctg gataaagtgg aagcttaccg
     5341 ctgtggactg aagaaaataa aaaatgactt gaacttgaag aagtcgttgt tggccactat
     5401 gaagacagaa ctacagaaag cccagcagat ccactctcag acttcacagc agtatccact
     5461 ttatgatctg gacttgggca agttcggtga aaaagtcaca cagctgacag accgctggca
     5521 aaggatagat aaacagatcg actttagatt atgggacctg gagaaacaaa tcaagcaatt
     5581 gaggaattat cgtgataact atcaggcttt ctgcaagtgg ctctatgatg ctaaacgccg
     5641 ccaggattcc ttagaatcca tgaaatttgg agattccaac acagtcatgc ggtttttgaa
     5701 tgagcagaag aacttgcaca gtgaaatatc tggcaaacga gacaaatcag aggaagtaca
     5761 aaaaattgct gaactttgcg ccaattcaat taaggattat gagctccagc tggcctcata
     5821 cacctcagga ctggaaactc tgctgaacat acctatcaag aggaccatga ttcagtcccc
     5881 ttctggggtg attctgcaag aggctgcaga tgttcatgct cggtacattg aactacttac
     5941 aagatctgga gactattaca ggttcttaag tgagatgctg aagagtttgg aagatctgaa
     6001 gctgaaaaat accaagatcg aagttttgga agaggagctc agactggccc gagatgccaa
     6061 ctcggaaaac tgtaataaga acaaattcct ggatcagaac ctgcagaaat accaggcaga
     6121 gtgttcccag ttcaaagcga agcttgcgag cctggaggag ctgaagagac aggctgagct
     6181 ggatgggaag tcggctaagc aaaatctaga caagtgctac ggccaaataa aagaactcaa
     6241 tgagaagatc acccgactga cttatgagat tgaagatgaa aagagaagaa gaaaatctgt
     6301 ggaagacaga tttgaccaac agaagaatga ctatgaccaa ctgcagaaag caaggcaatg
     6361 tgaaaaggag aaccttggtt ggcagaaatt agagtctgag aaagccatca aggagaagga
     6421 gtacgagatt gaaaggttga gggttctact gcaggaagaa ggcacccgga agagagaata
     6481 tgaaaatgag ctggcaaagg catctaatag gattcaggaa tcaaagaatc agtgtactca
     6541 ggtggtacag gaaagagaga gccttctggt gaaaatcaaa gtcctggagc aagacaaggc
     6601 aaggctgcag aggctggagg atgagctgaa tcgtgcaaaa tcaactctag aggcagaaac
     6661 cagggtgaaa cagcgcctgg agtgtgagaa acagcaaatt cagaatgacc tgaatcagtg
     6721 gaagactcaa tattcccgca aggaggaggc tattaggaag atagaatcgg aaagagaaaa
     6781 gagtgagaga gagaagaaca gtcttaggag tgagatcgaa agactccaag cagagatcaa
     6841 gagaattgaa gagaggtgca ggcgtaagct ggaggattct accagggaga cacagtcaca
     6901 gttagaaaca gaacgctccc gatatcagag ggagattgat aaactcagac agcgcccata
     6961 tgggtcccat cgagaggtcg agatggtgag caaaggcgaa gagctgttca ccggcgtggt
     7021 gcccatcctg gtggagctgg acggcgacgt gaacggccac aagttcagcg tgagcggcga
     7081 gggcgagggc gacgccacct acggcaagct gaccctgaag ctgctgtgca ccaccggcaa
     7141 gctgcccgtg ccctggccca ccctggtgac caccctgggc tacggcgtgc agtgcttcgc
     7201 ccggtacccc gaccacatga agcagcacga cttcttcaag agcgccatgc ccgagggcta
     7261 cgtgcaggag cggaccatct tcttcaagga cgacggcaac tacaagaccc gggccgaggt
     7321 gaagttcgag ggcgacaccc tggtgaaccg gatcgagctg aagggcatcg acttcaagga
     7381 ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca acgtgtacat
     7441 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccggc acaacatcga
     7501 ggacggcggc gtgcagctgg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     7561 cgtgctgctg cccgacaacc actacctgag ctaccagagc gccctgttca aggaccccaa
     7621 cgagaagcgg gaccacatgg tgctgctgga gttcctgacc gccgccggat ccgggccagg
     7681 tggagcaggg ccaggtggtg cagggccagg tggtgcaggg ccaggtggtg cagggcccgg
     7741 tggtgcaggt ccaggtggtg caggtccagg tggtgctggt ccaggtggtg ctggatccat
     7801 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     7861 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     7921 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     7981 cgcctgggac atcctgtccc ctcagttcat gctcggctcc aaggcctacg tgaagcaccc
     8041 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     8101 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     8161 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     8221 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     8281 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     8341 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     8401 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     8461 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaag cggccgcctc
     8521 ggccaaacat cgatagcggc cgcctataca tcgataaaat aaaagatttt atttagtctc
     8581 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg
     8641 ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca
     8701 ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     8761 cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
     8821 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc
     8881 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga
     8941 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct
     9001 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt
     9061 gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg
     9121 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt
     9181 ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc
     9241 accgggaggt aagctggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     9301 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     9361 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     9421 agcgatagcg gagtgtactg ataacttcgt ataatgtatg ctatacgaag ttattaggtc
     9481 tgaagaggag tttacgtcca gccaagctag ggccgcgatc cggaaccctt aatataactt
     9541 cgtataatgt atgctatacg aagttatcag tactggctta actatgcggc atcagagcag
     9601 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     9661 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     9721 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     9781 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9841 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9901 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9961 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    10021 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    10081 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    10141 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    10201 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    10261 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
    10321 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    10381 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    10441 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    10501 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    10561 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    10621 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    10681 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    10741 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    10801 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    10861 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    10921 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    10981 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    11041 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    11101 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    11161 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    11221 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    11281 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    11341 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    11401 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    11461 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    11521 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    11581 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
    11641 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattgct agcaattgct
    11701 agcaattgct agcaattcat accagatcac cgaaaactgt cctccaaatg tgtccccctc
    11761 acactcccaa attcgcgggc ttctgcctct tagaccactc taccctattc cccacactca
    11821 ccggagccaa agccgcggcc cttccgtttc tttgct
//
