LOCUS       sequence-230481-       11824 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plamid was used for improving the production of DMAPP (IPP) in 
            E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11824)
  AUTHORS   He BB, Bu XL, Zhou T, Li SM, Xu MJ, Xu J
  TITLE     Combinatory Biosynthesis of Prenylated 4-Hydroxybenzoate Derivatives
            by Overexpression of the Substrate-Promiscuous Prenyltransferase 
            XimB in Engineered E. coli.
  JOURNAL   ACS Synth Biol. 2018 Sep 21;7(9):2094-2104. doi: 
            10.1021/acssynbio.8b00070. Epub 2018 Aug 30.
  PUBMED    30103600
REFERENCE   2  (bases 1 to 11824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00070"; journalName: "ACS Synth Biol"; date: 
            "2018-09-21- 21"; volume: "7"; issue: "9"; pages: "2094-2104"
COMMENT     Sequence Label: pMVA
FEATURES             Location/Qualifiers
     source          1..11824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             83..100
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3154..3165
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4728..4746
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4747..4771
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             8409..8453
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(8491..8509)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8505..8552
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(8723..9514)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(9515..9606)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9654..10392)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(10602..11684)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     11646..11665
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(11685..11762)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        join(11808..11824,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag
      121 ctcgatgtca ttaccgttct taacttctgc accgggaaag gttattattt ttggtgaaca
      181 ctctgctgtg tacaacaagc ctgccgtcgc tgctagtgtg tctgcgttga gaacctacct
      241 gctaataagc gagtcatctg caccagatac tattgaattg gacttcccgg acattagctt
      301 taatcataag tggtccatca atgatttcaa tgccatcacc gaggatcaag taaactccca
      361 aaaattggcc aaggctcaac aagccaccga tggcttgtct caggaactcg ttagtctttt
      421 ggatccgttg ttagctcaac tatccgaatc cttccactac catgcagcgt tttgtttcct
      481 gtatatgttt gtttgcctat gcccccatgc caagaatatt aagttttctt taaagtctac
      541 tttacccatc ggtgctgggt tgggctcaag cgcctctatt tctgtatcac tggccttagc
      601 tatggcctac ttgggggggt taataggatc taatgacttg gaaaagctgt cagaaaacga
      661 taagcatata gtgaatcaat gggccttcat aggtgaaaag tgtattcacg gtaccccttc
      721 aggaatagat aacgctgtgg ccacttatgg taatgccctg ctatttgaaa aagactcaca
      781 taatggaaca ataaacacaa acaattttaa gttcttagat gatttcccag ccattccaat
      841 gatcctaacc tatactagaa ttccaaggtc tacaaaagat cttgttgctc gcgttcgtgt
      901 gttggtcacc gagaaatttc ctgaagttat gaagccaatt ctagatgcca tgggtgaatg
      961 tgccctacaa ggcttagaga tcatgactaa gttaagtaaa tgtaaaggca ccgatgacga
     1021 ggctgtagaa actaataatg aactgtatga acaactattg gaattgataa gaataaatca
     1081 tggactgctt gtctcaatcg gtgtttctca tcctggatta gaacttatta aaaatctgag
     1141 cgatgatttg agaattggct ccacaaaact taccggtgct ggtggcggcg gttgctcttt
     1201 gactttgtta cgaagagaca ttactcaaga gcaaattgac agcttcaaaa agaaattgca
     1261 agatgatttt agttacgaga catttgaaac agacttgggt gggactggct gctgtttgtt
     1321 aagcgcaaaa aatttgaata aagatcttaa aatcaaatcc ctagtattcc aattatttga
     1381 aaataaaact accacaaagc aacaaattga cgatctatta ttgccaggaa acacgaattt
     1441 accatggact tcatagaaat aattttgttt aactttaaaa ggagatataa tgtcagagtt
     1501 gagagccttc agtgccccag ggaaagcgtt actagctggt ggatatttag ttttagatac
     1561 aaaatatgaa gcatttgtag tcggattatc ggcaagaatg catgctgtag cccatcctta
     1621 cggttcattg caagggtctg ataagtttga agtgcgtgtg aaaagtaaac aatttaaaga
     1681 tggggagtgg ctgtaccata taagtcctaa aagtggcttc attcctgttt cgataggcgg
     1741 atctaagaac cctttcattg aaaaagttat cgctaacgta tttagctact ttaaacctaa
     1801 catggacgac tactgcaata gaaacttgtt cgttattgat attttctctg atgatgccta
     1861 ccattctcag gaggatagcg ttaccgaaca tcgtggcaac agaagattga gttttcattc
     1921 gcacagaatt gaagaagttc ccaaaacagg gctgggctcc tcggcaggtt tagtcacagt
     1981 tttaactaca gctttggcct ccttttttgt atcggacctg gaaaataatg tagacaaata
     2041 tagagaagtt attcataatt tagcacaagt tgctcattgt caagctcagg gtaaaattgg
     2101 aagcgggttt gatgtagcgg cggcagcata tggatctatc agatatagaa gattcccacc
     2161 cgcattaatc tctaatttgc cagatattgg aagtgctact tacggcagta aactggcgca
     2221 tttggttgat gaagaagact ggaatattac gattaaaagt aaccatttac cttcgggatt
     2281 aactttatgg atgggcgata ttaagaatgg ttcagaaaca gtaaaactgg tccagaaggt
     2341 aaaaaattgg tatgattcgc atatgccaga aagcttgaaa atatatacag aactcgatca
     2401 tgcaaattct agatttatgg atggactatc taaactagat cgcttacacg agactcatga
     2461 cgattacagc gatcagatat ttgagtctct tgagaggaat gactgtacct gtcaaaagta
     2521 tcctgaaatc acagaagtta gagatgcagt tgccacaatt agacgttcct ttagaaaaat
     2581 aactaaagaa tctggtgccg atatcgaacc tcccgtacaa actagcttat tggatgattg
     2641 ccagacctta aaaggagttc ttacttgctt aatacctggt gctggtggtt atgacgccat
     2701 tgcagtgatt actaagcaag atgttgatct tagggctcaa accgctaatg acaaaagatt
     2761 ttctaaggtt caatggctgg atgtaactca ggctgactgg ggtgttagga aagaaaaaga
     2821 tccggaaact tatcttgata aatagccatc ttagtatatt agttaagtat aagaaggaga
     2881 tataatgacc gtttacacag catccgttac cgcacccgtc aacatcgcaa cccttaagta
     2941 ttgggggaaa agggacacga agttgaatct gcccaccaat tcgtccatat cagtgacttt
     3001 atcgcaagat gacctcagaa cgttgacctc tgcggctact gcacctgagt ttgaacgcga
     3061 cactttgtgg ttaaatggag aaccacacag catcgacaat gaaagaactc aaaattgtct
     3121 gcgcgaccta cgccaattaa gaaaggaaat ggaatcgaag gacgcctcat tgcccacatt
     3181 atctcaatgg aaactccaca ttgtctccga aaataacttt cctacagcag ctggtttagc
     3241 ttcctccgct gctggctttg ctgcattggt ctctgcaatt gctaagttat accaattacc
     3301 acagtcaact tcagaaatat ctagaatagc aagaaagggg tctggttcag cttgtagatc
     3361 gttgtttggc ggatacgtgg cctgggaaat gggaaaagct gaagatggtc atgattccat
     3421 ggcagtacaa atcgcagaca gctctgactg gcctcagatg aaagcttgtg tcctagttgt
     3481 cagcgatatt aaaaaggatg tgagttccac tcagggtatg caattgaccg tggcaacctc
     3541 cgaactattt aaagaaagaa ttgaacatgt cgtaccaaag agatttgaag tcatgcgtaa
     3601 agccattgtt gaaaaagatt tcgccacctt tgcaaaggaa acaatgatgg attccaactc
     3661 tttccatgcc acatgtttgg actctttccc tccaatattc tacatgaatg acacttccaa
     3721 gcgtatcatc agttggtgcc acaccattaa tcagttttac ggagaaacaa tcgttgcata
     3781 cacgtttgat gcaggtccaa atgctgtgtt gtactactta gctgaaaatg agtcgaaact
     3841 ctttgcattt atctataaat tgtttggctc tgttcctgga tgggacaaga aatttactac
     3901 tgagcagctt gaggctttca accatcaatt tgaatcatct aactttactg cacgtgaatt
     3961 ggatcttgag ttgcaaaagg atgttgccag agtgatttta actcaagtcg gttcaggccc
     4021 acaagaaaca aacgaatctt tgattgacgc aaagactggt ctaccaaagg aataattctc
     4081 catacccgtt ttttgggcta acaaggagat taaatgcaaa cggaacacgt cattttattg
     4141 aatgcacagg gagttcccac gggtacgctg gaaaagtatg ccgcacacac ggcagacacc
     4201 cgcttacatc tcgcgttctc cagttggctg tttaatgcca aaggacaatt attagttacc
     4261 cgccgcgcac tgagcaaaaa agcatggcct ggcgtgtgga ctaactcggt ttgtgggcac
     4321 ccacaactgg gagaaagcaa cgaagacgca gtgatccgcc gttgccgtta tgagcttggc
     4381 gtggaaatta cgcctcctga atctatctat cctgactttc gctaccgcgc caccgatccg
     4441 agtggcattg tggaaaatga agtgtgtccg gtatttgccg cacgcaccac tagtgcgtta
     4501 cagatcaatg atgatgaagt gatggattat caatggtgtg atttagcaga tgtattacac
     4561 ggtattgatg ccacgccgtg ggcgttcagt ccgtggatgg tgatgcaggc gacaaatcgc
     4621 gaagccagaa aacgattatc tgcatttacc cagcttaaat aagcggccgc ataatgctta
     4681 agtcgaacag aaagtaatcg tattgtacac ggccgcataa tcgaaattaa tacgactcac
     4741 tataggggaa ttgtgagcgg ataacaattc cccatcttag tatattagtt aagtataaga
     4801 aggagatata catatgaaga cggtagttat tatcgacgca ctgcgtaccc ccattggaaa
     4861 atacaaagga agtctgagcc aggtaagcgc cgtcgacctg ggcacacatg tgaccacgca
     4921 gttgttgaag cgtcacagca ctatcagcga ggaaattgat caggtcattt ttggtaatgt
     4981 tctgcaggcg ggcaatgggc agaaccctgc acgtcagatt gcaatcaact caggtttaag
     5041 ccatgaaatt ccagcgatga cggtcaatga ggtctgtggc agtgggatga aagcggtaat
     5101 cctggccaaa cagttaatcc agctgggtga ggcggaggta cttatcgcag gtggtattga
     5161 aaacatgtca caggccccga aactgcaacg ctttaactac gaaacagaaa gctacgatgc
     5221 gcctttttcg tccatgatgt atgatggtct taccgacgca ttcagtggtc aggcgatggg
     5281 tctgacggcc gagaatgttg ctgaaaaata ccacgttacc cgtgaggaac aagaccaatt
     5341 ctctgtccat agccaactca aagcggcaca ggctcaggca gaaggcattt ttgccgatga
     5401 gattgcacca ctggaagttt ccggcaccct ggtggaaaag gacgagggca ttcgtccgaa
     5461 tagcagtgtt gaaaaactcg gtactttgaa aaccgtattc aaagaggacg gcacggtgac
     5521 tgccggtaat gcctcaacta tcaacgacgg tgcctcggca ctgattattg cgtctcaaga
     5581 atacgcggaa gcgcacggct tgccgtatct cgcgattatc cgcgattcag tggaggtcgg
     5641 catcgatccc gcgtacatgg gcatttcgcc gatcaaagca attcagaagc ttctggcacg
     5701 caaccagttg acgaccgaag agattgattt atacgaaatc aatgaagcgt tcgcggcgac
     5761 ctcgattgtg gttcagcgtg aacttgccct cccggaagaa aaggtcaaca tctatggcgg
     5821 aggcatcagt ttgggccatg ccatcggagc gaccggtgcc cgtctgctca ccagcttatc
     5881 atatcagttg aaccagaaag aaaaaaagta cggcgttgca tctctgtgta ttggcggagg
     5941 tctgggcctc gccatgttgt tagaacgtcc gcagcaaaaa aaaaactccc gcttttatca
     6001 gatgtcgccg gaggaacgtc tggcgagctt gttgaacgaa gggcagatct ctgccgacac
     6061 taaaaaggaa ttcgaaaaca cggcactgag cagtcagatt gcgaaccata tgattgaaaa
     6121 tcagatcagc gagaccgagg tgcccatggg cgtgggcctt catctcacgg tggacgaaac
     6181 ggattatctg gtaccaatgg ccacagaaga accgtcggta atcgccgcgt tgtcaaatgg
     6241 cgcgaaaatc gcgcaagggt tcaaaacggt caaccagcag cgtctcatgc gcggccagat
     6301 cgtgttctat gatgtagcag atgcagagag tctgattgac gagttacagg ttcgtgagac
     6361 ggagattttt cagcaagccg agctgtcgta cccgagcatt gttaaacgtg gcggtggcct
     6421 tcgtgacttg cagtatcgcg ccttcgacga atcgttcgtg agtgtcgact ttctggtaga
     6481 cgtgaaggac gccatggggg ccaatatcgt taatgccatg ctggaagggg ttgcagagct
     6541 gtttcgtgag tggttcgccg aacaaaaaat cctgtttagc atcttaagca attacgcaac
     6601 ggaaagcgtc gtgaccatga aaaccgcgat ccctgttagc cgcctttcaa agggcagtaa
     6661 cggtcgtgaa atcgctgaaa aaattgttct cgcgtcccgc tatgcatcgt tggatcctta
     6721 tcgcgcggtg acacacaaca aaggcattat gaatggtatc gaagcggtcg ttctggcgac
     6781 cggcaacgat actcgcgccg tgagcgcgtc ctgccatgct tttgctgtga aagagggccg
     6841 ttatcagggc ttgacgtcct ggaccctgga cggtgaacag ctgatcggcg aaatctcggt
     6901 gcccctcgcc ctggccactg tgggcggcgc cacaaaagtg ttgccaaaaa gccaagcggc
     6961 ggcggatctg ctggccgtaa ctgatgctaa ggaactgagt cgcgtggttg ccgcagtggg
     7021 cctggcccaa aacctggcag cactgcgcgc gctggtttct gaaggcatcc agaaaggtca
     7081 tatggccctg caagcgcgct ctctggccat gaccgtaggg gcgaccggca aggaagtcga
     7141 agcggtagct caacagttaa aacgccagaa aactatgaat caggatcgtg cgctggccat
     7201 cctcaatgac ctgcgcaaac agtaacttta agaaggagaa tatcatgacc attgggattg
     7261 ataaaatctc gtttttcgtg cctccttatt atatcgacat gacggccctg gccgaggctc
     7321 gcaatgtgga tcccggcaaa tttcacatcg gtatcggcca ggaccaaatg gcggtgaatc
     7381 ccatctcgca ggacattgtc accttcgccg caaacgcagc agaagctatc ttgactaaag
     7441 aagataaaga ggccatcgac atggtgatcg tgggtacgga aagctctatt gacgaaagta
     7501 aagccgcggc ggtggtatta caccgcctga tgggtatcca gccgtttgcg cgctcctttg
     7561 aaatcaaaga ggcctgctac ggcgcaacgg ctggactgca actcgcgaag aaccatgttg
     7621 cattacatcc ggataaaaaa gtcctggttg tcgcggcgga catcgcgaaa tacggcctga
     7681 actccggcgg ggaaccaacg cagggtgccg gcgcagtggc gatgcttgtc gcaagcgagc
     7741 ctcgtatcct ggctttaaag gaggacaacg tgatgctgac acaggatatt tacgattttt
     7801 ggcgtcccac cggtcatcca tatccgatgg ttgatggtcc tctgtccaat gaaacttata
     7861 ttcagagctt cgcgcaagtt tgggatgaac ataagaaacg taccggtctg gattttgcgg
     7921 attacgacgc tctggctttt cacattccat acacgaaaat gggcaaaaaa gccctcttag
     7981 ctaaaatctc agaccagacc gaggcagaac aggaacgcat tttagcgcgt tacgaagagt
     8041 caattatcta cagccgccgt gtaggtaatt tatatacggg gtcgctttat ctgggattga
     8101 tttccttact cgaaaacgcc acaaccctga cggcgggtaa ccaaatcggt ttattctctt
     8161 acggtagcgg tgccgttgcc gaattcttca cgggtgagct ggttgccggt taccagaacc
     8221 acttacagaa agaaacccac ctcgccctgc tggacaaccg tactgaactc agcatcgcag
     8281 aatatgaggc catgttcgcc gaaacactcg acacggatat cgatcaaacc ttagaggatg
     8341 aactcaaata ttccatttca gcgattaata acaccgtccg ctcctatcgc aattagctcg
     8401 agtctggtaa agaaaccgct gctgcgaaat ttgaacgcca gcacatggac tcgtctacta
     8461 gcgcagctta attaacctag gctgctgcca ccgctgagca ataactagca taaccccttg
     8521 gggcctctaa acgggtcttg aggggttttt tgctgaaacc tcaggcattt gagaagcaca
     8581 cggtcacact gcttccggta gtcaataaac cggtaaacca gcaatagaca taagcggcta
     8641 tttaacgacc ctgccctgaa ccgacgaccg ggtcatcgtg gccggatctt gcggcccctc
     8701 ggcttgaacg aattgttaga cattatttgc cgactacctt ggtgatctcg cctttcacgt
     8761 agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa
     8821 gataagcctg tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg
     8881 cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc
     8941 gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata
     9001 gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt
     9061 cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag
     9121 ccagatcaat gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc
     9181 attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca
     9241 caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt
     9301 ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa
     9361 ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat
     9421 gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt
     9481 gagtcgatac ttcggcgatc accgcttccc tcatactctt cctttttcaa tattattgaa
     9541 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9601 aacaaatagc tagctcactc ggtcgctacg ctccgggcgt gagactgcgg cgggcgctgc
     9661 ggacacatac aaagttaccc acagattccg tggataagca ggggactaac atgtgaggca
     9721 aaacagcagg gccgcgccgg tggcgttttt ccataggctc cgccctcctg ccagagttca
     9781 cataaacaga cgcttttccg gtgcatctgt gggagccgtg aggctcaacc atgaatctga
     9841 cagtacgggc gaaacccgac aggacttaaa gatccccacc gtttccggcg ggtcgctccc
     9901 tcttgcgctc tcctgttccg accctgccgt ttaccggata cctgttccgc ctttctccct
     9961 tacgggaagt gtggcgcttt ctcatagctc acacactggt atctcggctc ggtgtaggtc
    10021 gttcgctcca agctgggctg taagcaagaa ctccccgttc agcccgactg ctgcgcctta
    10081 tccggtaact gttcacttga gtccaacccg gaaaagcacg gtaaaacgcc actggcagca
    10141 gccattggta actgggagtt cgcagaggat ttgtttagct aaacacgcgg ttgctcttga
    10201 agtgtgcgcc aaagtccggc tacactggaa ggacagattt ggttgctgtg ctctgcgaaa
    10261 gccagttacc acggttaagc agttccccaa ctgacttaac cttcgatcaa accacctccc
    10321 caggtggttt tttcgtttac agggcaaaag attacgcgca gaaaaaaagg atctcaagaa
    10381 gatcctttga tcttttctac tgaaccgctc tagatttcag tgcaatttat ctcttcaaat
    10441 gtagcacctg aagtcagccc catacgatat aagttgtaat tctcatgtta gtcatgcccc
    10501 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg
    10561 gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt
    10621 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    10681 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg
    10741 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc
    10801 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta
    10861 tcgtcgtatc ccactaccga gatgtccgca ccaacgcgca gcccggactc ggtaatggcg
    10921 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc
    10981 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt
    11041 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga
    11101 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg
    11161 accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg
    11221 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca
    11281 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg
    11341 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc
    11401 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc
    11461 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt
    11521 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc
    11581 cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag
    11641 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat
    11701 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg
    11761 tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaaattaa tacgactcac
    11821 tata
//
