LOCUS       sequence-230482-        9920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoter expression plasmid for human codon optimized AsBE1.4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9920)
  AUTHORS   Kleinstiver BP, Sousa AA, Walton RT, Tak YE, Hsu JY, Clement K, 
            Welch MM, Horng JE, Malagon-Lopez J, Scarfo I, Maus MV, Pinello L, 
            Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas12a variants with increased activities and 
            improved targeting ranges for gene, epigenetic and base editing.
  JOURNAL   Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0.
  PUBMED    30742127
REFERENCE   2  (bases 1 to 9920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0."
COMMENT     Sequence Label: 
            pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-gs-UGI-NLS(SV
            40)(AsBE1.4) (RTW1008)
FEATURES             Location/Qualifiers
     source          1..9920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          163..1179
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1103..1125
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1187..1206
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1272..1281
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1284..1304
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1314..1334
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1344..2027
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             2124..6041
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     CDS             6072..6320
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6333..6353
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6443..6462)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6508..6563
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6562..6581)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6924..6940)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6924..6940)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6937..6959)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6948..6964
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6972..6978,6979..6996,6997..7002))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7017..7038
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7097..7292
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7143..7278
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7298..7432
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7335..7354)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7389..7408
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7500..7517)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7671..8259)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7751..7770)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8430..9221,9222..9290))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9053..9072
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9291..9395)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        9426..9805
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggggggggcg cgccrggggg gggggggggg ggsgrggggs ggggsggggs grggcggrgr
       61 ggkgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
      121 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc
      181 cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
      241 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc
      301 ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg
      361 gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg
      421 agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg
      481 ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc
      541 ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggkgagca
      601 gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct
      661 gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg
      721 ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg
      781 gagggctcgg gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg
      841 agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc
      901 caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg
      961 gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg
     1021 cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt
     1081 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc
     1141 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
     1201 attgtgctgt ctcatcattt tggcaaagaa ttctgcagtc gacggtaccg cgggcccggg
     1261 atccaccggt cgccaccatg ggcccaaaga aaaagaggaa agtcggcagt ggacctaaaa
     1321 agaaacgaaa ggttgggtca ggtagctcag agactggccc agtggctgtg gaccccacat
     1381 tgagacggcg gatcgagccc catgagtttg aggtattctt cgatccgaga gagctccgca
     1441 aggagacctg cctgctttac gaaattaatt gggggggccg gcactccatt tggcgacata
     1501 catcacagaa cactaacaag cacgtcgaag tcaacttcat cgagaagttc acgacagaaa
     1561 gatatttctg tccgaacaca aggtgcagca ttacctggtt tctcagctgg agcccatgcg
     1621 gcgaatgtag tagggccatc actgaattcc tgtcaaggta tccccacgtc actctgttta
     1681 tttacatcgc aaggctgtac caccacgctg acccccgcaa tcgacaaggc ctgcgggatt
     1741 tgatctcttc aggtgtgact atccaaatta tgactgagca ggagtcagga tactgctgga
     1801 gaaactttgt gaattatagc ccgagtaatg aagcccactg gcctaggtat ccccatctgt
     1861 gggtacgact gtacgttctt gaactgtact gcatcatact gggcctgcct ccttgtctca
     1921 acattctgag aaggaagcag ccacagctga cattctttac catcgctctt cagtcttgtc
     1981 attaccagcg actgccccca cacattctct gggccaccgg gttgaaatct ggtggttctt
     2041 ctggtggttc tagcggcagc gagactcccg ggacctcaga gtccgccaca cccgaaagtt
     2101 ccggagggag tagcggcggg tctacacagt tcgagggctt taccaacctg tatcaggtga
     2161 gcaagacact gcggtttgag ctgatcccac agggcaagac cctgaagcac atccaggagc
     2221 agggcttcat cgaggaggac aaggcccgca atgatcacta caaggagctg aagcccatca
     2281 tcgatcggat ctacaagacc tatgccgacc agtgcctgca gctggtgcag ctggattggg
     2341 agaacctgag cgccgccatc gactcctata gaaaggagaa aaccgaggag acaaggaacg
     2401 ccctgatcga ggagcaggcc acatatcgca atgccatcca cgactacttc atcggccgga
     2461 cagacaacct gaccgatgcc atcaataaga gacacgccga gatctacaag ggcctgttca
     2521 aggccgagct gtttaatggc aaggtgctga agcagctggg caccgtgacc acaaccgagc
     2581 acgagaacgc cctgctgcgg agcttcgaca agtttacaac ctacttctcc ggcttttatg
     2641 agaacaggaa gaacgtgttc agcgccgagg atatcagcac agccatccca caccgcatcg
     2701 tgcaggacaa cttccccaag tttaaggaga attgtcacat cttcacacgc ctgatcaccg
     2761 ccgtgcccag cctgcgggag cactttgaga acgtgaagaa ggccatcggc atcttcgtga
     2821 gcacctccat cgaggaggtg ttttccttcc ctttttataa ccagctgctg acacagaccc
     2881 agatcgacct gtataaccag ctgctgggag gaatctctcg ggaggcaggc accgagaaga
     2941 tcaagggcct gaacgaggtg ctgaatctgg ccatccagaa gaatgatgag acagcccaca
     3001 tcatcgcctc cctgccacac agattcatcc ccctgtttaa gcagatcctg tccgatagga
     3061 acaccctgtc tttcatcctg gaggagttta agagcgacga ggaagtgatc cagtccttct
     3121 gcaagtacaa gacactgctg agaaacgaga acgtgctgga gacagccgag gccctgttta
     3181 acgagctgaa cagcatcgac ctgacacaca tcttcatcag ccacaagaag ctggagacaa
     3241 tcagcagcgc cctgtgcgac cactgggata cactgaggaa tgccctgtat gagcggagaa
     3301 tctccgagct gacaggcaag atcaccaagt ctgccaagga gaaggtgcag cgcagcctga
     3361 agcacgagga tatcaacctg caggagatca tctctgccgc aggcaaggag ctgagcgagg
     3421 ccttcaagca gaaaaccagc gagatcctgt cccacgcaca cgccgccctg gatcagccac
     3481 tgcctacaac cctgaagaag caggaggaga aggagatcct gaagtctcag ctggacagcc
     3541 tgctgggcct gtaccacctg ctggactggt ttgccgtgga tgagtccaac gaggtggacc
     3601 ccgagttctc tgcccggctg accggcatca agctggagat ggagccttct ctgagcttct
     3661 acaacaaggc cagaaattat gccaccaaga agccctactc cgtggagaag ttcaagctga
     3721 actttcagat gcctacactg gcctctggct gggacgtgaa taaggagaag aacaatggcg
     3781 ccatcctgtt tgtgaagaac ggcctgtact atctgggcat catgccaaag cagaagggca
     3841 ggtataaggc cctgagcttc gagcccacag agaaaaccag cgagggcttt gataagatgt
     3901 actatgacta cttccctgat gccgccaaga tgatcccaaa gtgcagcacc cagctgaagg
     3961 ccgtgacagc ccactttcag acccacacaa cccccatcct gctgtccaac aatttcatcg
     4021 agcctctgga gatcacaaag gagatctacg acctgaacaa tcctgagaag gagccaaaga
     4081 agtttcagac agcctacgcc aagaaaaccg gcgaccagaa gggctacaga gaggccctgt
     4141 gcaagtggat cgacttcaca agggattttc tgtccaagta taccaagaca acctctatcg
     4201 atctgtctag cctgcggcca tcctctcagt ataaggacct gggcgagtac tatgccgagc
     4261 tgaatcccct gctgtaccac atcagcttcc agagaatcgc cgagaaggag atcatggatg
     4321 ccgtggagac aggcaagctg tacctgttcc agatctataa caaggacttt gccaagggcc
     4381 accacggcaa gcctaatctg cacacactgt attggaccgg cctgttttct ccagagaacc
     4441 tggccaagac aagcatcaag ctgaatggcc aggccgagct gttctaccgc cctaagtcca
     4501 ggatgaagag gatggcacac cggctgggag agaagatgct gaacaagaag ctgaaggatc
     4561 agaaaacccc aatccccgac accctgtacc aggagctgta cgactatgtg aatcacagac
     4621 tgtcccacga cctgtctgat gaggccaggg ccctgctgcc caacgtgatc accaaggagg
     4681 tgtctcacga gatcatcaag gataggcgct ttaccagcga caagttcttt ttccacgtgc
     4741 ctatcacact gaactatcag gccgccaatt ccccatctaa gttcaaccag agggtgaatg
     4801 cctacctgaa ggagcacccc gagacaccta tcatcggcat cgcccggggc gagagaaacc
     4861 tgatctatat cacagtgatc gactccaccg gcaagatcct ggagcagcgg agcctgaaca
     4921 ccatccagca gtttgattac cagaagaagc tggacaacag ggagaaggag agggtggcag
     4981 caaggcaggc ctggtctgtg gtgggcacaa tcaaggatct gaagcagggc tatctgagcc
     5041 aggtcatcca cgagatcgtg gacctgatga tccactacca ggccgtggtg gtgctggaga
     5101 acctgaattt cggctttaag agcaagagga ccggcatcgc cgagaaggcc gtgtaccagc
     5161 agttcgagaa gatgctgatc gataagctga attgcctggt gctgaaggac tatccagcag
     5221 agaaagtggg aggcgtgctg aacccatacc agctgacaga ccagttcacc tcctttgcca
     5281 agatgggcac ccagtctggc ttcctgtttt acgtgcctgc cccatataca tctaagatcg
     5341 atcccctgac cggcttcgtg gaccccttcg tgtggaaaac catcaagaat cacgagagcc
     5401 gcaagcactt cctggagggc ttcgactttc tgcactacga cgtgaaaacc ggcgacttca
     5461 tcctgcactt taagatgaac agaaatctgt ccttccagag gggcctgccc ggctttatgc
     5521 ctgcatggga tatcgtgttc gagaagaacg agacacagtt tgacgccaag ggcacccctt
     5581 tcatcgccgg caagagaatc gtgccagtga tcgagaatca cagattcacc ggcagatacc
     5641 gggacctgta tcctgccaac gagctgatcg ccctgctgga ggagaagggc atcgtgttca
     5701 gggatggctc caacatcctg ccaaagctgc tggagaatga cgattctcac gccatcgaca
     5761 cgatggtggc cctgatccgc agcgtgctgc agatgcggaa ctccaatgcc gccacaggcg
     5821 aggactatat caacagcccc gtgcgcgatc tgaatggcgt gtgcttcgac tcccggtttc
     5881 agaacccaga gtggccaatg gacgccgatg ccaatggcgc ctaccacatc gccctgaagg
     5941 gccagctgct gctgaatcac ctgaaggaga gcaaggatct gaagctgcag aacggcatct
     6001 ccaatcagga ctggctggcc tacatccagg agctgcgcaa ctctggtggt tctggaggat
     6061 ctggtggttc tactaatctg tcagatatta ttgaaaagga gaccggtaag caactggtta
     6121 tccaggaatc catcctcatg ctcccagagg aggtggaaga agtcattggg aacaagccgg
     6181 aaagcgatat actcgtgcac accgcctacg acgagagcac cgacgagaat gtcatgcttc
     6241 tgactagcga cgcccctgaa tacaagcctt gggctctggt catacaggat agcaacggtg
     6301 agaacaagat taagatgctc tctggtggtt ctcccaagaa gaagaggaaa gtctaaagcg
     6361 gccgcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
     6421 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
     6481 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
     6541 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
     6601 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
     6661 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
     6721 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
     6781 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
     6841 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
     6901 agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     6961 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7021 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7081 tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     7141 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     7201 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     7261 gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg cagcttataa
     7321 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7381 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgctgcat
     7441 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7501 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7561 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7621 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7681 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7741 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7801 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7861 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7921 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7981 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     8041 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     8101 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8161 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8221 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8281 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8341 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8401 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8461 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8521 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8581 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8641 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8701 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8761 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8821 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8881 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8941 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     9001 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     9061 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9121 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9181 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9241 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9301 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9361 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9421 gggtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     9481 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     9541 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     9601 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     9661 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     9721 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     9781 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     9841 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     9901 agcgatgggg gcgggggggg
//
