LOCUS       sequence-230528-        2401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GBPart - Operator/DNA Response element - 2 copies of the p53 DNA 
            binding motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: p2xp53_RE
FEATURES             Location/Qualifiers
     source          1..2401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..36
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     20..36
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(203..221)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(204..221)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(237..253)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(237..253)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(368..956)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(448..467)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1071..1173
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1174..1833
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1240..1259)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1946..2374
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2033..2052)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2243..2264
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacattgta gaaacgcatg taaaacgacg gccagtgaat tgtaatacga ctcactatag
       61 ggtgaattcc gcttaggacc ttagatattg atctcaaggt ctcggggaag gcaaggccag
      121 gcaagtccag gcaaggcaca gcatgcttag acatgttctg tactcccgag accactcatc
      181 gcccaatcac tagtgaattc gttctatagt gtcacctaaa taccatagag ccgttggtca
      241 tagctgtttc ctgtccaggc atcatgacac ggttatccac agaatcaggg gataacgcag
      301 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      361 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      421 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      481 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      541 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      601 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      661 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      721 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      781 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
      841 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      901 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
      961 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1021 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttgtga tgatcggcac
     1081 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
     1141 ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     1201 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     1261 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     1321 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     1381 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     1441 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     1501 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     1561 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtgtc agccaatccc
     1621 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     1681 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     1741 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     1801 cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct
     1861 taaacgcctg gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg
     1921 atgataagct gtcaaacatg atgttacgcg ccctgtagcg gcgcattaag cgcggcgggt
     1981 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     2041 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     2101 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     2161 tagggtgatg gttcacgtag tgggccagcg ccctgataga cggtttttcg ccctttgacg
     2221 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     2281 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     2341 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     2401 t
//
