LOCUS       sequence-230534-       13466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The no force control for the F40-based human desmoplakin I tension 
            sensor serves to detect changes in FRET between mTFP1 and mEYFP that
            are tension-independent..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13466)
  AUTHORS   Price AJ, Cost AL, Ungewiss H, Waschke J, Dunn AR, Grashoff C
  TITLE     Mechanical loading of desmosomes depends on the magnitude and 
            orientation of external stress.
  JOURNAL   Nat Commun. 2018 Dec 11;9(1):5284. doi: 10.1038/s41467-018-07523-0.
  PUBMED    30538252
REFERENCE   2  (bases 1 to 13466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-11"; volume: "9(1):5284. doi"; pages: " 
            10.1038/s41467-018-07523-0"
COMMENT     Sequence Label: Desmoplakin I no force control (F40-based)
FEATURES             Location/Qualifiers
     source          1..13466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        295..607
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    303..321
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    339..357
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    374..392
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    410..428
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    446..464
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    481..499
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    517..535
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     580..604
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6928..7632
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="VSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDGT
                     NTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTFE
                     DKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLKG
                     DVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESAV
                     ARNSTDGMDELYK"
     CDS             join(7753..7755,7756..7758,7759..8469)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7798..7819)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8059..8078)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8406..8427
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8602..8723
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8613..8632)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8667..8686
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             join(9446..10063,10064..10069,10070..10105,10106..10108,
                     10109..10144,10145..10147,10148..10183,10184..10189)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             10196..10252
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10253..10852
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10253..10272)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10749..10769
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(10907..10924)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10913..11137
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(11463..11480)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11634..12222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11714..11733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12393..13184,13185..13253))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13016..13035
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13254..13358)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggatccctat acagttgaag tcggaagttt acatacactt aagttggagt cattaaaact
       61 cgtttttcaa ctactccaca aatttcttgt taacaaacaa tagttttggc aagtcagtta
      121 ggacatctac tttgtgcatg acacaagtca tttttccaac aattgtttac agacagatta
      181 tttcacttat aattcactgt atcacaattc cagtgggtca gaagtttaca tacactaagt
      241 tcgactcctc tgcagaatgc ggcgatgttt cggtaagggg tccgctatct agacgagttt
      301 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
      361 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt
      421 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta
      481 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta
      541 tgtcgattag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag tgaaccgtca
      601 gatcgcctgg agccaattcc aactcttttg ccttatacct actttccgta ccacttccta
      661 ccctcgaaag gcctctgcgg ccgcgtcaac agcccctgct ccttgggccc ctccatgcca
      721 tgccgtaatc tctcccaccc gaccaacacc aacacccagc tccgacgcag ctcctctgcg
      781 cccttgccgc cctccgagcc acagctttcc tcccgctcct gcccccggcc cgtcgccgtc
      841 tccgcgctcg cagcggcctc gggagggccc aggtagcgag cagcgacctc gcgagccttc
      901 cgcactcccg cccggttccc cggccgtccg cctatccttg gccccctccg ctttctccgc
      961 gccggcccgc ctcgcttatg cctcggcgct gagccgctct cccgattgcc cgccgacatg
     1021 agctgcaacg gaggctccca cccgcggatc aacactctgg gccgcatgat ccgcgccgag
     1081 tctggcccgg acctgcgcta cgaggtgacc agcggcggcg ggggcaccag caggatgtac
     1141 tattctcggc gcggcgtgat caccgaccag aactcggacg gctactgtca aaccggcacg
     1201 atgtccaggc accagaacca gaacaccatc caggagctgc tgcagaactg ctccgactgc
     1261 ttgatgcgag cagagctcat cgtgcagcct gaattgaagt atggagatgg aatacaactg
     1321 actcggagtc gagaattgga tgagtgtttt gcccaggcca atgaccaaat ggaaatcctc
     1381 gacagcttga tcagagagat gcggcagatg ggccagccct gtgatgctta ccagaaaagg
     1441 cttcttcagc tccaagagca aatgcgagcc ctttataaag ccatcagtgt ccctcgagtc
     1501 cgcagggcca gctccaaggg tggtggaggc tacacttgtc agagtggctc tggctgggat
     1561 gagttcacca aacatgtcac cagtgaatgt ttggggtgga tgaggcagca aagggcggag
     1621 atggacatgg tggcctgggg tgtggacctg gcctcagtgg agcagcacat taacagccac
     1681 cggggcatcc acaactccat cggcgactat cgctggcagc tggacaaaat caaagccgac
     1741 ctgcgcgaga aatctgcgat ctaccagttg gaggaggagt atgaaaacct gctgaaagcg
     1801 tcctttgaga ggatggatca cctgcgacag ctgcagaaca tcattcaggc cacgtccagg
     1861 gagatcatgt ggatcaatga ctgcgaggag gaggagctgc tgtacgactg gagcgacaag
     1921 aacaccaaca tcgctcagaa acaggaggcc ttctccatac gcatgagtca actggaagtt
     1981 aaagaaaaag agctcaataa gctgaaacaa gaaagtgacc aacttgtcct caatcagcat
     2041 ccagcttcag acaaaattga ggcctatatg gacactctgc agacgcagtg gagttggatt
     2101 cttcagatca ccaagtgcat tgatgttcat ctgaaagaaa atgctgccta ctttcagttt
     2161 tttgaagagg cgcagtctac tgaagcatac ctgaaggggc tccaggactc catcaggaag
     2221 aagtacccct gcgacaagaa catgcccctg cagcacctgc tggaacagat caaggagctg
     2281 gagaaagaac gagagaaaat ccttgaatac aagcgtcagg tgcagaactt ggtaaacaag
     2341 tctaagaaga ttgtacagct gaagcctcgt aacccagact acagaagcaa taaacccatt
     2401 attctcagag ctctctgtga ctacaaacaa gatcagaaaa tcgtgcataa gggggatgag
     2461 tgtatcctga aggacaacaa cgagcgcagc aagtggtacg tgacgggccc gggaggcgtt
     2521 gacatgcttg ttccctctgt ggggctgatc atccctcctc cgaacccact ggccgtggac
     2581 ctctcttgca agattgagca gtactacgaa gccatcttgg ctctgtggaa ccagctctac
     2641 atcaacatga agagcctggt gtcctggcac tactgcatga ttgacataga gaagatcagg
     2701 gccatgacaa tcgccaagct gaaaacaatg cggcaggaag attacatgaa gacgatagcc
     2761 gaccttgagt tacattacca agagttcatc agaaatagcc aaggctcaga gatgtttgga
     2821 gatgatgaca agcggaaaat acagtctcag ttcaccgatg cccagaagca ttaccagacc
     2881 ctggtcattc agctccctgg ctatccccag caccagacag tgaccacaac tgaaatcact
     2941 catcatggaa cctgccaaga tgtcaaccat aataaagtaa ttgaaaccaa cagagaaaat
     3001 gacaagcaag aaacatggat gctgatggag ctgcagaaga ttcgcaggca gatagagcac
     3061 tgcgagggca ggatgactct caaaaacctc cctctagcag accaggggtc ttctcaccac
     3121 atcacagtga aaattaacga gcttaagagt gtgcagaatg attcacaagc aattgctgag
     3181 gttctcaacc agcttaaaga tatgcttgcc aacttcagag gttctgaaaa gtactgctat
     3241 ttacagaatg aagtatttgg actatttcag aaactggaaa atatcaatgg tgttacagat
     3301 ggctacttaa atagcttatg cacagtaagg gcactgctcc aggctattct ccaaacagaa
     3361 gacatgttaa aggtttatga agccaggctc actgaggagg aaactgtctg cctggacctg
     3421 gataaagtgg aagcttaccg ctgtggactg aagaaaataa aaaatgactt gaacttgaag
     3481 aagtcgttgt tggccactat gaagacagaa ctacagaaag cccagcagat ccactctcag
     3541 acttcacagc agtatccact ttatgatctg gacttgggca agttcggtga aaaagtcaca
     3601 cagctgacag accgctggca aaggatagat aaacagatcg actttagatt atgggacctg
     3661 gagaaacaaa tcaagcaatt gaggaattat cgtgataact atcaggcttt ctgcaagtgg
     3721 ctctatgatg ctaaacgccg ccaggattcc ttagaatcca tgaaatttgg agattccaac
     3781 acagtcatgc ggtttttgaa tgagcagaag aacttgcaca gtgaaatatc tggcaaacga
     3841 gacaaatcag aggaagtaca aaaaattgct gaactttgcg ccaattcaat taaggattat
     3901 gagctccagc tggcctcata cacctcagga ctggaaactc tgctgaacat acctatcaag
     3961 aggaccatga ttcagtcccc ttctggggtg attctgcaag aggctgcaga tgttcatgct
     4021 cggtacattg aactacttac aagatctgga gactattaca ggttcttaag tgagatgctg
     4081 aagagtttgg aagatctgaa gctgaaaaat accaagatcg aagttttgga agaggagctc
     4141 agactggccc gagatgccaa ctcggaaaac tgtaataaga acaaattcct ggatcagaac
     4201 ctgcagaaat accaggcaga gtgttcccag ttcaaagcga agcttgcgag cctggaggag
     4261 ctgaagagac aggctgagct ggatgggaag tcggctaagc aaaatctaga caagtgctac
     4321 ggccaaataa aagaactcaa tgagaagatc acccgactga cttatgagat tgaagatgaa
     4381 aagagaagaa gaaaatctgt ggaagacaga tttgaccaac agaagaatga ctatgaccaa
     4441 ctgcagaaag caaggcaatg tgaaaaggag aaccttggtt ggcagaaatt agagtctgag
     4501 aaagccatca aggagaagga gtacgagatt gaaaggttga gggttctact gcaggaagaa
     4561 ggcacccgga agagagaata tgaaaatgag ctggcaaagg taagaaacca ctataatgag
     4621 gagatgagta atttaaggaa caagtatgaa acagagatta acattacgaa gaccaccatc
     4681 aaggagatat ccatgcaaaa agaggatgat tccaaaaatc ttagaaacca gcttgataga
     4741 ctttcaaggg aaaatcgaga tctgaaggat gaaattgtca ggctcaatga cagcatcttg
     4801 caggccactg agcagcgaag gcgagctgaa gaaaacgccc ttcagcaaaa ggcctgtggc
     4861 tctgagataa tgcagaagaa gcagcatctg gagatagaac tgaagcaggt catgcagcag
     4921 cgctctgagg acaatgcccg gcacaagcag tccctggagg aggctgccaa gaccattcag
     4981 gacaaaaata aggagatcga gagactcaaa gctgagtttc aggaggaggc caagcgccgc
     5041 tgggaatatg aaaatgaact gagtaaggta agaaacaatt atgatgagga gatcattagc
     5101 ttaaaaaatc agtttgagac cgagatcaac atcaccaaga ccaccatcca ccagctcacc
     5161 atgcagaagg aagaggatac cagtggctac cgggctcaga tagacaatct cacccgagaa
     5221 aacaggagct tatctgaaga aataaagagg ctgaagaaca ctctaaccca gaccacagag
     5281 aatctcagga gggtggaaga agacatccaa cagcaaaagg ccactggctc tgaggtgtct
     5341 cagaggaaac agcagctgga ggttgagctg agacaagtca ctcagatgcg aacagaggag
     5401 agcgtaagat ataagcaatc tcttgatgat gctgccaaaa ccatccagga taaaaacaag
     5461 gagatagaaa ggttaaaaca actgatcgac aaagaaacaa atgaccggaa atgcctggaa
     5521 gatgaaaacg cgagattaca aagggtccag tatgacctgc agaaagcaaa cagtagtgcg
     5581 acggagacaa taaacaaact gaaggttcag gagcaagaac tgacacgcct gaggatcgac
     5641 tatgaaaggg tttcccagga gaggactgtg aaggaccagg atatcacgcg gttccagaac
     5701 tctctgaaag agctgcagct gcagaagcag aaggtggaag aggagctgaa tcggctgaag
     5761 aggaccgcgt cagaagactc ctgcaagagg aagaagctgg aggaagagct ggaaggcatg
     5821 aggaggtcgc tgaaggagca agccatcaaa atcaccaacc tgacccagca gctggagcag
     5881 gcatccattg ttaagaagag gagtgaggat gacctccggc agcagaggga cgtgctggat
     5941 ggccacctga gggaaaagca gaggacccag gaagagctga ggaggctctc ttctgaggtc
     6001 gaggccctga ggcggcagtt actccaggaa caggaaagtg tcaaacaagc tcacttgagg
     6061 aatgagcatt tccagaaggc gatagaagat aaaagcagaa gcttaaatga aagcaaaata
     6121 gaaattgaga ggctgcagtc tctcacagag aacctgacca aggagcactt gatgttagaa
     6181 gaagaactgc ggaacctgag gctggagtac gatgacctga ggagaggacg aagcgaagcg
     6241 gacagtgata aaaatgcaac catcttggaa ctaaggagcc agctgcagat cagcaacaac
     6301 cggaccctgg aactgcaggg gctgattaat gatttacaga gagagaggga aaatttgaga
     6361 caggaaattg agaaattcca aaagcaggct ttagaggcat ctaataggat tcaggaatca
     6421 aagaatcagt gtactcaggt ggtacaggaa agagagagcc ttctggtgaa aatcaaagtc
     6481 ctggagcaag acaaggcaag gctgcagagg ctggaggatg agctgaatcg tgcaaaatca
     6541 actctagagg cagaaaccag ggtgaaacag cgcctggagt gtgagaaaca gcaaattcag
     6601 aatgacctga atcagtggaa gactcaatat tcccgcaagg aggaggctat taggaagata
     6661 gaatcggaaa gagaaaagag tgagagagag aagaacagtc ttaggagtga gatcgaaaga
     6721 ctccaagcag agatcaagag aattgaagag aggtgcaggc gtaagctgga ggattctacc
     6781 agggagacac agtcacagtt agaaacagaa cgctcccgat atcagaggga gattgataaa
     6841 ctcagacagc gcttaattaa gggaagcggc ggaaccggat cgactagcgg gggtagcggg
     6901 ggaagcacgg ggggaggaac tggcgccgtg agcaagggcg aggagaccac aatgggcgta
     6961 atcaagcccg acatgaagat caagctgaag atggagggca acgtgaatgg ccacgccttc
     7021 gtgatcgagg gcgagggcga gggcaagccc tacgacggca ccaacaccat caacctggag
     7081 gtgaaggagg gagcccccct gcccttctcc tacgacattc tgaccaccgc gttcgcctac
     7141 ggcaacaggg ccttcaccaa gtaccccgac gacatcccca actacttcaa gcagtccttc
     7201 cccgagggct actcttggga gcgcaccatg accttcgagg acaagggcat cgtgaaggtg
     7261 aagtccgaca tctccatgga ggaggactcc ttcatctacg agatacacct caagggcgag
     7321 aacttccccc ccaacggccc cgtgatgcag aagaagacca ccggctggga cgcctccacc
     7381 gagaggatgt acgtgcgcga cggcgtgctg aagggcgacg tcaagcacaa gctgctgctg
     7441 gagggcggcg gccaccaccg cgttgacttc aagaccatct acagggccaa gaaggcggtg
     7501 aagctgcccg actatcactt tgtggaccac cgcatcgaga tcctgaacca cgacaaggac
     7561 tacaacaagg tgaccgttta cgagagcgcc gtggcccgca actccaccga cggcatggac
     7621 gagctgtaca aggggccagg tggtgcaggg ccaggtggtg cagggccagg tggtgcaggg
     7681 ccaggtggtg cagggcccgg tggtgcaggt ccaggtggtg caggtccagg tggtgcaggt
     7741 ccaggtggtg ctatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     7801 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     7861 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     7921 ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc
     7981 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     8041 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     8101 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     8161 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     8221 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     8281 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     8341 cccgacaacc actacctgag ctaccagtcc aaactgagca aagaccccaa cgagaagcgc
     8401 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     8461 ctgtacaagg gcaccggagg tggcactagc ggaggctcag gcggatcgac ctccggaact
     8521 ggaggttccg gtagcggccg ctgaataggt aaggcgcgcc gctagcggcc tgtcaggcca
     8581 agcttccatc gatagacatg ataagataca ttgatgagtt tggacaaacc acaacaagaa
     8641 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     8701 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     8761 agggggagat gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtacctagg
     8821 cgagaccctg tctcacaaaa taaagtaagc ccggactgag tgcggaaagg cgggcctggc
     8881 gggtctggtc tccccatgcg ggccaccaga ggccctgcag ccttcagtcg cttgaagggg
     8941 taatggcgct tccactcaca aacatggcgg acagagcgtg tgaacgagat gaacagcccc
     9001 tcaaaaatat ggccgccgag gctggacggc cgtgccccag cagcaccgcc tccgcgcccc
     9061 acgtgatctc tcgccgggca cagcgctgac cgcggaggtc caaccggaag aatgtccgga
     9121 ttggacattc ggaagagggc ccgccttccc tggggaatct ctgcgcacgc gcagaacgct
     9181 tcgaccaatg aaaacacagg aagccgtccg cgcaaccgcg ttgcgtcact tctgccgccc
     9241 ctgtttcaag gtatatagcc gtagacggaa cttcgccttt ctctcggcct tagcgccatt
     9301 tttttgggtg agtgtttttt ggttcctgcg ttgggattcc gtgtacaatc catagacatc
     9361 tgacctcggc acttagcatc atcacagcaa actaactgta gcctttctct ctttccctgt
     9421 agaaacctct gcacctgagg ccaccatgtc tagactggac aagagcaaag tcataaacgg
     9481 cgctctggaa ttactcaatg gagtcggtat cgaaggcctg acgacaagga aactcgctca
     9541 aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct
     9601 cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg
     9661 cgagtcatgg caagactttc tgcggaacaa cgccaagtca ttccgctgtg ctctcctctc
     9721 acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac
     9781 cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta
     9841 cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca
     9901 agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca
     9961 agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact
    10021 aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc ggcgggccgg ccgacgccct
    10081 tgacgatttt gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat
    10141 gctgcctgct gacgctcttg acgattttga ccttgacatg ctccccgggt ccggcgctac
    10201 taacttcagc ctgctgaagc aggctggtga cgtcgaggag aatcctggtc ccatgaccga
    10261 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
    10321 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
    10381 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
    10441 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
    10501 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
    10561 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
    10621 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
    10681 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
    10741 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
    10801 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gattcgaagg
    10861 cctgtcgtga agcttgggga tcaattctct agagctcgct gatcagcctc gactgtgcct
    10921 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
    10981 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
    11041 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
    11101 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
    11161 tgggcctact agctactcgg gaccccttac cgaaacatcg ccgcattctg cagaggagtc
    11221 gagtgtatgt aaacttctga cccactggga atgtgatgaa agaaataaaa gctgaaatga
    11281 atcattctct ctactattat tctgatattt cacattctta aaataaagtg gtgatcctaa
    11341 ctgacctaag acagggaatt tttactagga ttaaatgtca ggaattgtga aaaagtgagt
    11401 ttaaatgtat ttggctaagg tgtatgtaaa cttccgactt caactgtata gggatccgct
    11461 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    11521 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    11581 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    11641 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    11701 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    11761 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    11821 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    11881 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    11941 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    12001 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    12061 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    12121 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    12181 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    12241 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    12301 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    12361 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    12421 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    12481 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    12541 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    12601 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    12661 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    12721 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    12781 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    12841 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    12901 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    12961 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    13021 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    13081 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    13141 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    13201 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    13261 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    13321 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    13381 cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt
    13441 gtaagcgtta atattttgtt aaaatt
//
