LOCUS       sequence-230536-        9921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoter expression plasmid for human codon optimized enAsBE1.4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9921)
  AUTHORS   Kleinstiver BP, Sousa AA, Walton RT, Tak YE, Hsu JY, Clement K, 
            Welch MM, Horng JE, Malagon-Lopez J, Scarfo I, Maus MV, Pinello L, 
            Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas12a variants with increased activities and 
            improved targeting ranges for gene, epigenetic and base editing.
  JOURNAL   Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0.
  PUBMED    30742127
REFERENCE   2  (bases 1 to 9921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0."
COMMENT     Sequence Label: 
            pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/
            D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028)
FEATURES             Location/Qualifiers
     source          1..9921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          663..1679
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1603..1625
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1687..1706
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1772..1781
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1784..1804
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1814..1834
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1844..2527
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             2624..6541
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     CDS             6572..6820
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6833..6853
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6943..6962)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7008..7063
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7062..7081)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7424..7440)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7424..7440)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7437..7459)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7448..7464
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7472..7478,7479..7496,7497..7502))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7517..7538
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7597..7792
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7643..7778
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7798..7932
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7835..7854)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7889..7908
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8000..8017)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8171..8759)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8251..8270)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8930..9721,9722..9790))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9553..9572
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9791..9895)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggnsgcg cgccrggsgg ggsggggsgg ggcgaggggs
      541 ggggsggggs grggsggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgkggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttctgcagtc
     1741 gacggtaccg cgggcccggg atccaccggt cgccaccatg ggcccaaaga aaaagaggaa
     1801 agtcggcagt ggacctaaaa agaaacgaaa ggttgggtca ggtagctcag agactggccc
     1861 agtggctgtg gaccccacat tgagacggcg gatcgagccc catgagtttg aggtattctt
     1921 cgatccgaga gagctccgca aggagacctg cctgctttac gaaattaatt gggggggccg
     1981 gcactccatt tggcgacata catcacagaa cactaacaag cacgtcgaag tcaacttcat
     2041 cgagaagttc acgacagaaa gatatttctg tccgaacaca aggtgcagca ttacctggtt
     2101 tctcagctgg agcccatgcg gcgaatgtag tagggccatc actgaattcc tgtcaaggta
     2161 tccccacgtc actctgttta tttacatcgc aaggctgtac caccacgctg acccccgcaa
     2221 tcgacaaggc ctgcgggatt tgatctcttc aggtgtgact atccaaatta tgactgagca
     2281 ggagtcagga tactgctgga gaaactttgt gaattatagc ccgagtaatg aagcccactg
     2341 gcctaggtat ccccatctgt gggtacgact gtacgttctt gaactgtact gcatcatact
     2401 gggcctgcct ccttgtctca acattctgag aaggaagcag ccacagctga cattctttac
     2461 catcgctctt cagtcttgtc attaccagcg actgccccca cacattctct gggccaccgg
     2521 gttgaaatct ggtggttctt ctggtggttc tagcggcagc gagactcccg ggacctcaga
     2581 gtccgccaca cccgaaagtt ccggagggag tagcggcggg tctacacagt tcgagggctt
     2641 taccaacctg tatcaggtga gcaagacact gcggtttgag ctgatcccac agggcaagac
     2701 cctgaagcac atccaggagc agggcttcat cgaggaggac aaggcccgca atgatcacta
     2761 caaggagctg aagcccatca tcgatcggat ctacaagacc tatgccgacc agtgcctgca
     2821 gctggtgcag ctggattggg agaacctgag cgccgccatc gactcctata gaaaggagaa
     2881 aaccgaggag acaaggaacg ccctgatcga ggagcaggcc acatatcgca atgccatcca
     2941 cgactacttc atcggccgga cagacaacct gaccgatgcc atcaataaga gacacgccga
     3001 gatctacaag ggcctgttca aggccgagct gtttaatggc aaggtgctga agcagctggg
     3061 caccgtgacc acaaccgagc acgagaacgc cctgctgcgg agcttcgaca agtttacaac
     3121 ctacttctcc ggcttttata gaaacaggaa gaacgtgttc agcgccgagg atatcagcac
     3181 agccatccca caccgcatcg tgcaggacaa cttccccaag tttaaggaga attgtcacat
     3241 cttcacacgc ctgatcaccg ccgtgcccag cctgcgggag cactttgaga acgtgaagaa
     3301 ggccatcggc atcttcgtga gcacctccat cgaggaggtg ttttccttcc ctttttataa
     3361 ccagctgctg acacagaccc agatcgacct gtataaccag ctgctgggag gaatctctcg
     3421 ggaggcaggc accgagaaga tcaagggcct gaacgaggtg ctgaatctgg ccatccagaa
     3481 gaatgatgag acagcccaca tcatcgcctc cctgccacac agattcatcc ccctgtttaa
     3541 gcagatcctg tccgatagga acaccctgtc tttcatcctg gaggagttta agagcgacga
     3601 ggaagtgatc cagtccttct gcaagtacaa gacactgctg agaaacgaga acgtgctgga
     3661 gacagccgag gccctgttta acgagctgaa cagcatcgac ctgacacaca tcttcatcag
     3721 ccacaagaag ctggagacaa tcagcagcgc cctgtgcgac cactgggata cactgaggaa
     3781 tgccctgtat gagcggagaa tctccgagct gacaggcaag atcaccaagt ctgccaagga
     3841 gaaggtgcag cgcagcctga agcacgagga tatcaacctg caggagatca tctctgccgc
     3901 aggcaaggag ctgagcgagg ccttcaagca gaaaaccagc gagatcctgt cccacgcaca
     3961 cgccgccctg gatcagccac tgcctacaac cctgaagaag caggaggaga aggagatcct
     4021 gaagtctcag ctggacagcc tgctgggcct gtaccacctg ctggactggt ttgccgtgga
     4081 tgagtccaac gaggtggacc ccgagttctc tgcccggctg accggcatca agctggagat
     4141 ggagccttct ctgagcttct acaacaaggc cagaaattat gccaccaaga agccctactc
     4201 cgtggagaag ttcaagctga actttcagat gcctacactg gccagaggct gggacgtgaa
     4261 tagagagaag aacaatggcg ccatcctgtt tgtgaagaac ggcctgtact atctgggcat
     4321 catgccaaag cagaagggca ggtataaggc cctgagcttc gagcccacag agaaaaccag
     4381 cgagggcttt gataagatgt actatgacta cttccctgat gccgccaaga tgatcccaaa
     4441 gtgcagcacc cagctgaagg ccgtgacagc ccactttcag acccacacaa cccccatcct
     4501 gctgtccaac aatttcatcg agcctctgga gatcacaaag gagatctacg acctgaacaa
     4561 tcctgagaag gagccaaaga agtttcagac agcctacgcc aagaaaaccg gcgaccagaa
     4621 gggctacaga gaggccctgt gcaagtggat cgacttcaca agggattttc tgtccaagta
     4681 taccaagaca acctctatcg atctgtctag cctgcggcca tcctctcagt ataaggacct
     4741 gggcgagtac tatgccgagc tgaatcccct gctgtaccac atcagcttcc agagaatcgc
     4801 cgagaaggag atcatggatg ccgtggagac aggcaagctg tacctgttcc agatctataa
     4861 caaggacttt gccaagggcc accacggcaa gcctaatctg cacacactgt attggaccgg
     4921 cctgttttct ccagagaacc tggccaagac aagcatcaag ctgaatggcc aggccgagct
     4981 gttctaccgc cctaagtcca ggatgaagag gatggcacac cggctgggag agaagatgct
     5041 gaacaagaag ctgaaggatc agaaaacccc aatccccgac accctgtacc aggagctgta
     5101 cgactatgtg aatcacagac tgtcccacga cctgtctgat gaggccaggg ccctgctgcc
     5161 caacgtgatc accaaggagg tgtctcacga gatcatcaag gataggcgct ttaccagcga
     5221 caagttcttt ttccacgtgc ctatcacact gaactatcag gccgccaatt ccccatctaa
     5281 gttcaaccag agggtgaatg cctacctgaa ggagcacccc gagacaccta tcatcggcat
     5341 cgcccggggc gagagaaacc tgatctatat cacagtgatc gactccaccg gcaagatcct
     5401 ggagcagcgg agcctgaaca ccatccagca gtttgattac cagaagaagc tggacaacag
     5461 ggagaaggag agggtggcag caaggcaggc ctggtctgtg gtgggcacaa tcaaggatct
     5521 gaagcagggc tatctgagcc aggtcatcca cgagatcgtg gacctgatga tccactacca
     5581 ggccgtggtg gtgctggaga acctgaattt cggctttaag agcaagagga ccggcatcgc
     5641 cgagaaggcc gtgtaccagc agttcgagaa gatgctgatc gataagctga attgcctggt
     5701 gctgaaggac tatccagcag agaaagtggg aggcgtgctg aacccatacc agctgacaga
     5761 ccagttcacc tcctttgcca agatgggcac ccagtctggc ttcctgtttt acgtgcctgc
     5821 cccatataca tctaagatcg atcccctgac cggcttcgtg gaccccttcg tgtggaaaac
     5881 catcaagaat cacgagagcc gcaagcactt cctggagggc ttcgactttc tgcactacga
     5941 cgtgaaaacc ggcgacttca tcctgcactt taagatgaac agaaatctgt ccttccagag
     6001 gggcctgccc ggctttatgc ctgcatggga tatcgtgttc gagaagaacg agacacagtt
     6061 tgacgccaag ggcacccctt tcatcgccgg caagagaatc gtgccagtga tcgagaatca
     6121 cagattcacc ggcagatacc gggacctgta tcctgccaac gagctgatcg ccctgctgga
     6181 ggagaagggc atcgtgttca gggatggctc caacatcctg ccaaagctgc tggagaatga
     6241 cgattctcac gccatcgaca cgatggtggc cctgatccgc agcgtgctgc agatgcggaa
     6301 ctccaatgcc gccacaggcg aggactatat caacagcccc gtgcgcgatc tgaatggcgt
     6361 gtgcttcgac tcccggtttc agaacccaga gtggccaatg gacgccgatg ccaatggcgc
     6421 ctaccacatc gccctgaagg gccagctgct gctgaatcac ctgaaggaga gcaaggatct
     6481 gaagctgcag aacggcatct ccaatcagga ctggctggcc tacatccagg agctgcgcaa
     6541 ctctggtggt tctggaggat ctggtggttc tactaatctg tcagatatta ttgaaaagga
     6601 gaccggtaag caactggtta tccaggaatc catcctcatg ctcccagagg aggtggaaga
     6661 agtcattggg aacaagccgg aaagcgatat actcgtgcac accgcctacg acgagagcac
     6721 cgacgagaat gtcatgcttc tgactagcga cgcccctgaa tacaagcctt gggctctggt
     6781 catacaggat agcaacggtg agaacaagat taagatgctc tctggtggtt ctcccaagaa
     6841 gaagaggaaa gtctaaagcg gccgcactcc tcaggtgcag gctgcctatc agaaggtggt
     6901 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
     6961 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
     7021 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     7081 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     7141 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
     7201 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     7261 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     7321 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     7381 atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg
     7441 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     7501 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     7561 ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat
     7621 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7681 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7741 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac
     7801 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7861 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7921 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7981 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8041 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8101 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8161 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8221 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8281 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8341 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8401 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8461 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8521 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8581 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8641 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8701 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8761 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8821 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8881 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8941 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9001 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9061 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9121 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9181 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9241 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9301 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9361 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9421 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9481 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9541 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9601 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9661 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9721 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9781 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9841 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9901 ttccccgaaa agtgccacct g
//
