LOCUS       sequence-230538-        4841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cdc42E7 3'UTR-boxB tagged for T3 in vitro transcription to use in 
            pulldown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4841)
  AUTHORS   Ciolli Mattioli C, Rom A, Franke V, Imami K, Arrey G, Terne M, 
            Woehler A, Akalin A, Ulitsky I, Chekulaeva M
  TITLE     Alternative 3' UTRs direct localization of functionally diverse 
            protein isoforms in neuronal compartments.
  JOURNAL   Nucleic Acids Res. 2018 Dec 22. pii: 5258023. doi: 
            10.1093/nar/gky1270.
  PUBMED    30590745
REFERENCE   2  (bases 1 to 4841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Dec 22. pii: 5258023. doi: 10.1093/nar/gky1270."
COMMENT     Sequence Label: pMCh1807_pBS_cdc42E7-boxB
FEATURES             Location/Qualifiers
     source          1..4841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..125
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(126..194,195..986)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(344..363)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1157..1745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1646..1665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1899..1916
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2033..2054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2069..2074,2075..2092,2093..2099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2107..2123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2112..2134
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2131..2147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2131..2147
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2168..2186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    3666..3684
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    3708..3726
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    3732..3750
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    3790..3808
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    3814..3832
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     primer_bind     complement(4149..4165)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4149..4165)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4197..4216)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4198..4216)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4223..4240)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4223..4239)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4232..4254)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4381..4836
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4468..4487)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4678..4699
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     2101 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     2161 agctcgaaat taaccctcac taaagggaac aaaagctggt accgggcccc ccctcgagac
     2221 gcatctccag agccctttct gcacagctgg tgttgtcgtc atactaaaag caatgtttaa
     2281 atcaaactaa agattaaaaa ttaaaatttg tttctgcaat aatgacaaac gccctgcacc
     2341 tacccacatg cactcatgtg agacaaggcc cattagtatg gcccctcctt gcccttccag
     2401 taatagttaa ttttgaataa ttatgtattg tctgaaaagc aatgagtgct agtttttgtt
     2461 ttctttgttt aaaaaaaaaa acaaaaatcc ccacaaacgt ttgttgtttt tttgtttaaa
     2521 agcaagcatg cttgtattga ctgtaacaga ctaattcaga ttgtcaaagc tgctccctgg
     2581 ttccattttg gagagtaatc tggggcatct tcgtgtcttc tgttttgttt tgttttgttt
     2641 ttcctcaacc cttctctcct tttggggtgt gtatggggtt gtttcttaga cttgtatttt
     2701 aaaatttgtt aaccatagga cagcctgtgg ggaggaggac agagtatttc cacattccac
     2761 tccctaggtc tagtttagca aacgtgtccc cacctggtgc tcttaggaag gagtatagaa
     2821 acgcctcgat taacaacgta ttccttcttg aaagttgcct tttctctcca cctctgagtg
     2881 ggtccagtct gtgatggaac tcatgaaggt gggtggacgc tgtctcaccc ctctttctag
     2941 gatgcactct atatgtgact gtgactttca agggtatttg tttgccattt cctgatttag
     3001 ggaggggaat aatttctaac ttctttcatt gataaatgaa aagtattgca cctttgaaat
     3061 acaccaaatg gattgagttc ctaattaaaa aaagttcttt tgcccctgtc agtcattttc
     3121 ttacatgctt agcacagatt cacaactcac tgtgtagtgt tcctagattc agccgaagac
     3181 ctggtaccta gggttcctaa cgggatggtg ttaggaaaca catcatggct gatgattgcc
     3241 atctcctttc aggtgatgag gtcacaggcc tgcttcctta agaagctatg agcatggggt
     3301 ggggagcata cttttaacca acatggggac cagtcagggc tatccctttt tttcttccat
     3361 gcctatgagg aacctttgaa caactggctg aagctttcgc aagaaaaatg gcgagagcct
     3421 catgattctt tataagtgtt aatagagact ttatcagatt attttgtttg caggttccag
     3481 tttttaaaaa atgtttaggt aatctccttc cccagtcctg atcttgtgga ttcgttagtg
     3541 tcgaaccaat gctttcttgt gtctcaactc tttgtatatg aattcttttc agatgtatta
     3601 aacaaacaaa acccttcaca agtgagctag cttccctaag tccaactacc aaactgggga
     3661 ttcctgggcc ctgaagaagg gcccctcgac tagtccaaat acaaactggg ccctgaagaa
     3721 gggcccatat agggccctga agaagggccc tatcgaggat attatctcga ctaagtccaa
     3781 ctacaaactg ggccctgaag aagggcccat atagggccct gaagaagggc cctatcgagg
     3841 atattatcga aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3901 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3961 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4021 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4081 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aatgcataag cttgatatcg aattcctgca
     4141 gcccggggga tccactagtt ctagagcggc cgccaccgcg gtggagctcc aattcgccct
     4201 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     4261 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     4321 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     4381 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4441 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4501 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4561 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     4621 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4681 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4741 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4801 acgcgaattt taacaaaata ttaacgctta caatttaggt g
//
