LOCUS       sequence-23054-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Cummins JM, He Y, Leary RJ, Pagliarini R, Diaz LA, Sjoblom T, Barad 
            O, Bentwich Z, Szafranska AE, Labourier E, Raymond CK, Roberts BS, 
            Juhl H, Kinzler KW, Vogelstein B, Velculescu VE
  TITLE     The colorectal microRNAome.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Mar 7. 103(10):3687-92.
  PUBMED    16505370
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Mar 7. 103(10):3687-92."
COMMENT     Sequence Label: DICER KO vector
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    162..210
                     /note="synthetic polyadenylation signal"
     protein_bind    273..306
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gaggcggcga gggctgncgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
       61 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
      121 gaccgaccaa gcgacgccca acctgccatc acgatggccg caataaaata tctttatttt
      181 cattacatct gtgtgttggt tttttgtgtg aatcgatagc gataaggatc cgcaagggcg
      241 aattatcaag cttaggatcc ggaaccctta atataacttc gtataatgta tgctatacga
      301 agttattagg tccctcgaag aggttcacta gttctagagt gtttgatgag tgtcatcttg
      361 caatcctaga ccacccctat cgagaaatta tgaaggtaag ttttagacct aagtcttagt
      421 aattcttggt aaactattga gtaaatgcag atttttgttt ttagatttca aaagcaataa
      481 tattagttat caatattggg agtttggctg cagtgtatga atgaatgaat attaaattaa
      541 tgttcattta aatctataat aaacttttaa acatcataat aaattctata taattttcat
      601 agtgtatcta attgagcctt tttgaaaaac ngatctttta anccaaacta cagtaattct
      661 atgagtaatt ttatgtggtc cttggtnaag ggcaatttcc accaacaatc ctctttntac
      721 tatttgtagg aattgggttt ggtatgtttt aatatggcga tgtctctctt gttatagcct
      781 tgcaaatgaa gtcnttactg tcagtatctt tattatgtna gctctgtgaa attgtca
//
