LOCUS       sequence-230544-        2399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GBPart - Operator/DNA Response element - 5 copies of the NF-kb DNA 
            binding motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: p5xNF-kb_RE
FEATURES             Location/Qualifiers
     source          1..2399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..36
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     20..36
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(201..219)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(202..219)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(235..251)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(235..251)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(366..954)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(446..465)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1069..1171
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1172..1831
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1238..1257)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1944..2372
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2031..2050)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2241..2262
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacattgta gaaacgcatg taaaacgacg gccagtgaat tgtaatacga ctcactatag
       61 ggtgaattcc gcttaggacc ttagatattg atctcaaggt ctcggggagg aaattcccgg
      121 aaagtccccg gaaattcccg gaaagtcccc ggaaattccc ctcccgagac cactcatcgc
      181 ccaatcacta gtgaattcgt tctatagtgt cacctaaata ccatagagcc gttggtcata
      241 gctgtttcct gtccaggcat catgacacgg ttatccacag aatcagggga taacgcagga
      301 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      361 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      421 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      481 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      541 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      601 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      661 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      721 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      781 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
      841 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
      901 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
      961 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1021 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttgtgatg atcggcacgt
     1081 aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt
     1141 atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
     1201 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
     1261 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
     1321 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
     1381 tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
     1441 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
     1501 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
     1561 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtgtcag ccaatccctg
     1621 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
     1681 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
     1741 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
     1801 gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta
     1861 aacgcctggt tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgat
     1921 gataagctgt caaacatgat gttacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     1981 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     2041 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     2101 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     2161 gggtgatggt tcacgtagtg ggccagcgcc ctgatagacg gtttttcgcc ctttgacgtt
     2221 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     2281 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     2341 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattt
//
