LOCUS       sequence-230546-        4844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit for constitutive expression of ELuc in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: hCMV-IE1:ELuc
FEATURES             Location/Qualifiers
     source          1..4844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        86..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2310..2534
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2654..3242)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2734..2753)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3357..3459
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             3460..4275
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             3460..4275
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3533..3552)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4389..4817
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4476..4495)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4686..4707
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacaagctt cgtctcagtc aggagtagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
      601 tgaaccgtca gatcaagctc aaatgatgaa gagagagaag aatgttgtgt atgggcccga
      661 gcctaagcac ccgcttggga attttaccgc tggagagatg ctctacaacg cgcttcacaa
      721 acatagtcac atcccgcagg cgatcctcga cgtgatgggt aacgaaagtc ttagttatca
      781 ggaattcttc gacacgacgg tcaaattggg gcagagtctc cagaattgtg gctataaaat
      841 gaatgatgtt gtatcaatat gcgctgaaaa caacaaaaga tttttcattc caattattag
      901 tgcgtggtat ataggcatgg tcgttgcacc tgtaaacgag gactatatac cagacgaact
      961 gtgtaaggtc acaggaatta gtaagccaat acttgtgttc accacgcgaa agatacttcc
     1021 aaaggttttg gaggttaagg atagaacaaa ttatatcaaa agaatcataa ttctggactc
     1081 tgaggaaaac cttctcggat gtgaatcctt gcataatttt atgtcaagat atagcgataa
     1141 caatcttcag acctttaaac cactgcatta cgatccggtc gaccaggttg cagcgatact
     1201 gtgtagtagt ggtactaccg ggttgcctaa gggcgtcatg cagacccacc gcaatatatg
     1261 tgttcggctg acccacgcct ctgacccgag agttggtacc caactcattc ccggagttag
     1321 tgtgctggcg tatcttccct tctttcacgc ctttgggttt tccattaact tggggtattt
     1381 tatggtgggg ctgcgcgtcg tgatgcttcg gaggttcaac caagaggttt ttttgaaggc
     1441 gattcaagac tatgaagtac gaagtgtgat caatgtccca agtacaatcc tctttctttc
     1501 taaatcaccg ttggttgata agtatgatct ttctaccttg gcggaacttt gttgtggtgc
     1561 ggcacctctt gcaaaagaag tagctgaaat cgccgtaaaa cgccttaatt tgccaggcat
     1621 acggtgtggt tacgggttga ctgaaagcac atccgctaat atccacactc ttcacaacga
     1681 atttaaatcc ggttcattgg gaaaagtgac gccctacatg gccgctaaaa ttatagatcg
     1741 aaacaccgga gaggcgcttg gcccaaatca agtgggagag ttgtgcattt ggggccccat
     1801 ggtcacgaaa ggctacgtga ataatccaca ggccaccaag gaggccattg acgacgatgg
     1861 ttggcttcac agtggtgatt ttggctacta tgatgaggat gaatattttt acattgtgga
     1921 tagatataaa gagctgatca agtacaaggg atatcaagtg gctcctgtcg agttggagga
     1981 gattttgctc caacatccag ggatccgcga tgtggccgta gtcggtatcc ccgacataga
     2041 agcaggggaa cttccggccg gattcgttgt caagcaacct ggggcccaac tgacggctaa
     2101 agaagtatat gatttccttg cacaaagagt tagccacagc aagtatcttc gagggggtgt
     2161 cagattcgtt gactctattc cgaggaacgt cacgggcaaa atcagccgaa aggaactccg
     2221 agaagcactt atggagaagg cgggtggtgg atctatgtac aagacatccg ccgccaacga
     2281 tgagaactat gccctggcgg cttgagcttc tgtgccttct agttgccagc catctgttgt
     2341 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     2401 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     2461 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     2521 ggtgggctct atggcgcttg agacgaagct tgacacggtt atccacagaa tcaggggata
     2581 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2641 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2701 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2761 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2821 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2881 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2941 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3001 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3061 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     3121 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3181 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3241 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3301 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttgtgatgat
     3361 cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt
     3421 tttgagttat cgagattttc aggagctaag gaagctaaaa tgagccatat tcaacgggaa
     3481 acgtcttgct caaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     3541 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct accgattgta tgggaagccc
     3601 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     3661 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     3721 atccgtactc ctgatgatgc atggttactc accactgcga tcccagggaa aacagcattc
     3781 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     3841 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     3901 cgactcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     3961 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     4021 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     4081 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     4141 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     4201 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     4261 gatgagtttt tctgattttt tttaaggcag ttattggtgc ccttaaacgc ctggttgcta
     4321 cgcctgaata agtgataata agcggatgaa tggcagaaat tcgatgataa gctgtcaaac
     4381 atgatgttac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4441 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4501 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4561 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     4621 tagtgggcca gcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4681 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4741 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4801 aaaatttaac gcgaatttta acaaaatatt aacgcttaca attt
//
