LOCUS       sequence-230549-       11718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of mChe-tagged cdc42E7 under doxy-inducible CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11718)
  AUTHORS   Ciolli Mattioli C, Rom A, Franke V, Imami K, Arrey G, Terne M, 
            Woehler A, Akalin A, Ulitsky I, Chekulaeva M
  TITLE     Alternative 3' UTRs direct localization of functionally diverse 
            protein isoforms in neuronal compartments.
  JOURNAL   Nucleic Acids Res. 2018 Dec 22. pii: 5258023. doi: 
            10.1093/nar/gky1270.
  PUBMED    30590745
REFERENCE   2  (bases 1 to 11718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Dec 22. pii: 5258023. doi: 10.1093/nar/gky1270."
COMMENT     Sequence Label: pMCh1750_S2F-IMCg_doxy-CMV_mChe-Cdc42E7
FEATURES             Location/Qualifiers
     source          1..11718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             518..987
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1052..1409
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     primer_bind     1820..1842
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1858..1874
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    1927..1960
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     polyA_signal    2068..2202
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4366..5073)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4641..4660)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4910..4928
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    join(5224..5242,5243..5265,5266..5284,5285..5307,5308..5326,
                     5327..5349,5350..5368,5369..5391,5392..5410,5411..5433,
                     5434..5452,5453..5475,5476..5494)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        5509..5547
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             join(5645..5647,5648..5650,5651..6361)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5645..5647,5648..5650,5651..6361)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5690..5711)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5951..5970)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6298..6319
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7722..7843
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    7959..8006
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(9090..9107)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9090..9106)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9099..9121)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9315..9334)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9434..9456
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9494..9512)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9580..9684
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9685..9753,9754..10545)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9903..9922)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10716..11304
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11205..11224
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11458..11475
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11592..11613
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11628..11633,11634..11651,11652..11658)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11666..11682
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11671..11693
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11690..11706
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11690..11706
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagctagctt tgctcttagg agtttcctaa tacatcccaa actcaaatat ataaagcatt
       61 tgacttgttc tatgccctag ggggcggggg gaagctaagc cagctttttt taacatttaa
      121 aatgttaatt ccattttaaa tgcacagatg tttttatttc ataagggttt caatgtgcat
      181 gaatgctgca atattcctgt taccaaagct agtataaata aaaatagata aacgtggaaa
      241 ttacttagag tttctgtcat taacgtttcc ttcctcagtt gacaacataa atgcgctgct
      301 gagaagccag tttgcatctg tcaggatcaa tttcccatta tgccagtcat attaattact
      361 agtcaattag ttgattttta tttttgacat atacatgtga aagaccccac ctgtaggttt
      421 ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata
      481 gaaaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata
      541 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
      601 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
      661 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
      721 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
      781 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
      841 cggcgcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
      901 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      961 tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg gagacccctg
     1021 cccagggacc accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt
     1081 ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag ttagctaact
     1141 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt cggaacaccc ggccgcaacc
     1201 ctgggagacg tcccagggac ttcgggggcc gtttttgtgg cccgacctga gtcctaaaat
     1261 cccgatcgtt taggactctt tggtgcaccc cccttagagg agggatatgt ggttctggta
     1321 ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc ggtttgggac
     1381 cgaagccgcg ccgcgcgtct tgtctgctgc agcatcgttc tgtgttgtct ctgtctgact
     1441 gtgtttctgt atttgtctga aaatatgggc ccgggctagc ctgttaccac tcccttaagt
     1501 ttgaccttag gtcactggaa agatgtcgag cggatcgctc acaaccagtc ggtagatgtc
     1561 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
     1621 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
     1681 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
     1741 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttcct
     1801 ccatccgccc cgtctctccc ccttgaacct cctcgttcga ccccgcctcg atcctccctt
     1861 tatccagccc tcactccttc tctaggcgcc cccatatggc catatgagat ccgaagttcc
     1921 tattccgaag ttcctattct tcaaatagta taggaacttc agatcataat cagccatacc
     1981 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     2041 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     2101 taaggcaata gcatcacaaa tttcacaaat aaggcatttt tttcactgca ttctagtttt
     2161 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctcaaatcc ctcggaagct
     2221 gcgcctgtct taggttggag tgatacattt ttatcacttt tacccgtctt tggattaggc
     2281 agtagctctg acggccctcc tgtcttaggt tagtgaaaaa tgtcactctc ttacccgtca
     2341 ttggctgtcc agcttagctc gcaggggagg tggtctggat cctcacttgt gaagggtttt
     2401 gtttgtttaa tacatctgaa aagaattcat atacaaagag ttgagacaca agaaagcatt
     2461 ggttcgacac taacgaatcc acaagatcag gactggggaa ggagattacc taaacatttt
     2521 ttaaaaactg gaacctgcaa acaaaataat ctgataaagt ctctattaac acttataaag
     2581 aatcatgagg ctctcgccat ttttcttgcg aaagcttcag ccagttgttc aaaggttcct
     2641 cataggcatg gaagaaaaaa ggggatagcc ctgactggtc cccatgttgg ttaaaagtat
     2701 gctccccacc ccatgctcat agcttcttaa ggaagcaggc ctgtgacctc atcacctgaa
     2761 aggagatggc aatcatcagc catgatgtgt ttcctaacac catcccgtta ggaaccctag
     2821 gtaccaggtc ttcggctgaa tctaggaaca ctacacagtg agttgtgaat ctgtgctaag
     2881 catgtaagaa aatgactgac aggggcaaaa gaactttttt taattaggaa ctcaatccat
     2941 ttggtgtatt tcaaaggtgc aatacttttc atttatcaat gaaagaagtt agaaattatt
     3001 cccctcccta aatcaggaaa tggcaaacaa atacccttga aagtcacagt cacatataga
     3061 gtgcatccta gaaagagggg tgagacagcg tccacccacc ttcatgagtt ccatcacaga
     3121 ctggacccac tcagaggtgg agagaaaagg caactttcaa gaaggaatac gttgttaatc
     3181 gaggcgtttc tatactcctt cctaagagca ccaggtgggg acacgtttgc taaactagac
     3241 ctagggagtg gaatgtggaa atactctgtc ctcctcccca caggctgtcc tatggttaac
     3301 aaattttaaa atacaagtct aagaaacaac cccatacaca ccccaaaagg agagaagggt
     3361 tgaggaaaaa caaaacaaaa caaaacagaa gacacgaaga tgccccagat tactctccaa
     3421 aatggaacca gggagcagct ttgacaatct gaattagtct gttacagtca atacaagcat
     3481 gcttgctttt aaacaaaaaa acaacaaacg tttgtgggga tttttgtttt tttttttaaa
     3541 caaagaaaac aaaaactagc actcattgct tttcagacaa tacataatta ttcaaaatta
     3601 actattactg gaagggcaag gaggggccat actaatgggc cttgtctcac atgagtgcat
     3661 gtgggtaggt gcagggcgtt tgtcattatt gcagaaacaa attttaattt ttaatcttta
     3721 gtttgattta aacattgctt ttagtatgac gacaacacca gctgtgcaga aagggctctg
     3781 gagatgcgtt catagcagca cacacctgcg gctcttcttc ggttctggag gctccagggc
     3841 agccaatatt gcttcatcaa acacattctt taggcctttc tgtgtgaggg cggagcactc
     3901 cacatacttg acagccttca gatcccgcgc cagcttttca gcagtctctg gagtaatagg
     3961 cttctgtttg ttcttggcaa gtttctcaat agtagagggg tcatctctga gatcaatttg
     4021 ggtcccaaca agcaagaaag gagtctttgg acagtggtga gttatctcag gcacccactt
     4081 ttctttcaca ttttcaaatg aggatggaga gaccactgag aaacatacta gaaaaacatc
     4141 tgtctgtgga taacttagcg gtcgtagtct gtcataatcc tcttgccctg cagtatcaaa
     4201 aagtccaaga gtgtatggct ctccaccaat cataactgtg actgcatagt tgtcaaaaac
     4261 agttggtaca tattccgatg ggaatttgtt tgttgtgtag gatatcagga gacatgtttt
     4321 accaacagca ccatcaccaa caacaacaca cttaattgtc tgcatcttgt acagctcgtc
     4381 catgccgccg gtggagtggc ggccctcggc gcgttcgtac tgttccacga tggtgtagtc
     4441 ctcgttgtgg gaggtgatgt ccaacttgat gttgacgttg taggcgccgg gcagctgcac
     4501 gggcttcttg gccttgtagg tggtcttgac ctcagcgtcg tagtggccgc cgtccttcag
     4561 cttcagcctc tgcttgatct cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga
     4621 ggaggcctcc cagcccatgg tcttcttctg cattacgggg ccgtcggagg ggaagttggt
     4681 gccgcgcagc ttcaccttgt agatgaactc gccgtcctgc agggaggagt cctgggtcac
     4741 ggtcaccacg ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggggaa
     4801 ggacagcttc aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct tggagccgta
     4861 catgaactga ggggacagga tgtcccaggc gaagggcagg gggccaccct tggtcacctt
     4921 cagcttggcg gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa
     4981 ctcgtggccg ttcacggagc cctccatgtg caccttgaag cgcatgaact ccttgatgat
     5041 ggccatgtta tcctcctcgc ccttgctcac catggtgtcg accgcggagg ctggatcggt
     5101 cccggtgtct tctatggagg tcaaaacagc gtggatggcg tctccaggcg atctgacggt
     5161 tcactaaacg agctctgctt atataggcct cccaccgtac acgcctaaag cttctttcac
     5221 ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct atcactgata
     5281 gggagtggta aactcgactt tcacttttct ctatcactga tagggagtgg taaactcgac
     5341 tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca
     5401 ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa
     5461 ctcgactttc acttttctct atcactgata gggagtggta aactcgaggg taggcgtgta
     5521 cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     5581 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggaatt
     5641 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     5701 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     5761 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     5821 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     5881 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     5941 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     6001 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     6061 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     6121 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     6181 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     6241 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     6301 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     6361 gatgcagaca attaagtgtg ttgttgttgg tgatggtgct gttggtaaaa catgtctcct
     6421 gatatcctac acaacaaaca aattcccatc ggaatatgta ccaactgttt ttgacaacta
     6481 tgcagtcaca gttatgattg gtggagagcc atacactctt ggactttttg atactgcagg
     6541 gcaagaggat tatgacagac tacgaccgct aagttatcca cagacagatg tttttctagt
     6601 atgtttctca gtggtctctc catcctcatt tgaaaatgtg aaagaaaagt gggtgcctga
     6661 gataactcac cactgtccaa agactccttt cttgcttgtt gggacccaaa ttgatctcag
     6721 agatgacccc tctactattg agaaacttgc caagaacaaa cagaagccta ttactccaga
     6781 gactgctgaa aagctggcgc gggatctgaa ggctgtcaag tatgtggagt gctccgccct
     6841 cacacagaga ggtctgaaga atgtgtttga tgaggctatc ctagctgccc tcgagcctcc
     6901 ggaaactcaa cccaaaagga agtgctgtat attctaaacc gttttctcct tcccctcttt
     6961 gctgctgctt cctgtcccac tactgtagaa agatcattta aaacaaagga ataaaaccgt
     7021 cctgtttgaa agcctctgcg tcttcttact caacacttta gagcaaactc tgtattcatt
     7081 tttgatcatg aatgcaatta gaatattttt tgtgatcagt agtcacgtta gacttgttta
     7141 actttctgct gcttgagttg cctgatgctc agagcttttg gtttgaatta ttgcaaaagg
     7201 gaatgtggtc tggcattaag aatgatgtct tcttggagaa aataagagtt ttaacgcttc
     7261 tagatgttag ttctagatgg agaaagcatc actaaacatc attttactct tactgattgt
     7321 taattgtaat tgcatgacac actatgaaaa gggttggctt gctagtagag tgtaatgggg
     7381 aagggaggat tctcatggtt ttcctgtgtg cagtgaaact tttgtgttgc tcttgctttg
     7441 gctgtttgtg ccgtaggtgg agtgtttgtc attctgggat ggggaaggta tccatgtatc
     7501 tgcttactgc tttatgagtt cattgttata cattatctcc taatcctagc atccatttaa
     7561 caattgactt aagatttgct gagattgaaa tgtaaagcta ttgcctttat attttcctgc
     7621 ttttgattct attgttgtga gggaaacata cagttgtggt tatctttaca ttttgaaatt
     7681 aaaaatatac aattgtttgt ataaagcggc cgcagacatg ataagataca ttgatgagtt
     7741 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     7801 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     7861 tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct
     7921 ctacaaatgt ggtaaaatcc gataaggatc gatccggcga agttcctatt ccgaagttcc
     7981 tattctctag aaagtatagg aacttcagat ccggattagt ccaatttgtt aaagacagga
     8041 tatcagtggt ccaggctcta gttttgactc aacaatatca ccagctgaag cctatagagt
     8101 acgagccata gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
     8161 accccacctg taggtttggc aagctagctc gacttctgtt cgcgcgcttc tgctccccga
     8221 gctcgagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
     8281 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
     8341 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca catgcagcat
     8401 gtatcaaaat taatttggtt ttttttctta agtatttaca ttaaatggcc atagtactta
     8461 aagttacatt ggcttccttg aaataaacat ggagtattca gaatgtgtca taaatatttc
     8521 taattttaag atagtatctc cattggcttt ctactttttc ttttattttt ttttgtcctc
     8581 tgtcttccat ttgttgttgt tgttgtttgt ttgtttgttt gttggttggt tggttaattt
     8641 ttttttaaag atcctacact atagttcaag ctagactatt agctactctg taacccaggg
     8701 tgaccttgaa gtcatgggta gcctgctgtt ttagccttcc cacatctaag attacaggta
     8761 tgagctatca tttttggtat attgattgat tgattgattg atgtgtgtgt gtgtgattgt
     8821 gtttgtgtgt gtgattgtgt atatgtgtgt atggttgtgt gtgattgtgt gtatgtatgt
     8881 ttgtgtgtga ttgtgtgtgt gtgattgtgc atgtgtgtgt gtgtgattgt gtttatgtgt
     8941 atgattgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gttgtgtata
     9001 tatatttatg gtagtgagag gcaacgctcc ggctcaggtg tcaggttggt ttttgagaca
     9061 gagtctttca cttagcttgg aattaattca ctggccgtcg ttttacaacg tcgtgactgg
     9121 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     9181 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     9241 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     9301 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     9361 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     9421 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     9481 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     9541 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     9601 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     9661 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     9721 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     9781 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     9841 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     9901 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     9961 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
    10021 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
    10081 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
    10141 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
    10201 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
    10261 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
    10321 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
    10381 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
    10441 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
    10501 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
    10561 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    10621 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    10681 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    10741 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    10801 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    10861 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    10921 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    10981 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    11041 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    11101 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    11161 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    11221 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    11281 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    11341 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    11401 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    11461 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
    11521 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
    11581 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
    11641 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
    11701 tatgaccatg attacgcc
//
