LOCUS       sequence-230550-       10790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of mChe-tagged cdc42E7 under pSyn promoter for primary 
            neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Ciolli Mattioli C, Rom A, Franke V, Imami K, Arrey G, Terne M, 
            Woehler A, Akalin A, Ulitsky I, Chekulaeva M
  TITLE     Alternative 3' UTRs direct localization of functionally diverse 
            protein isoforms in neuronal compartments.
  JOURNAL   Nucleic Acids Res. 2018 Dec 22. pii: 5258023. doi: 
            10.1093/nar/gky1270.
  PUBMED    30590745
REFERENCE   2  (bases 1 to 10790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Dec 22. pii: 5258023. doi: 10.1093/nar/gky1270."
COMMENT     Sequence Label: pMCh2007_pLenti_Syn_mChe-cdc42E7
FEATURES             Location/Qualifiers
     source          1..10790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             3159..3866
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3304..3322)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3572..3591
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             6595..6775
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6801..6818)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6807..7031
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7077..7505
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7164..7183)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7374..7395
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7514..7534)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7519..7848
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7699..7834
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7761..7780
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7896..7943
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7962..8336
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8466..8587
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8503..8522)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8557..8576
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8636..8652)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8636..8652)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8649..8671)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8660..8676
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8684..8690,8691..8708,8709..8714))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8729..8750
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8867..8884)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9038..9626)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9118..9137)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9797..10588,10589..10657))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10420..10439
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10658..10762)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acaagtatct gggaagggta acaatgcaag ttagcctgag
     2641 gatttaggag gaggctgaaa aacagagtag gagccttact acgggtccag accctacgga
     2701 caagaacccc cactcccact ccccaaattg cgcattccct cccccatcag agggggaggg
     2761 gaagaggatg cagcgcggcg cggcgcgtgc gcactgtcgg atttagtacc gcggacagag
     2821 ccttcgcccc cgctgccggc gcgcgccacc acctccccag caccaaaggc gggctgacgt
     2881 cactctccag ccctccccaa actcccctac ctcaccgcct tggtcgcgtc cgtgcagcgg
     2941 tgagtccagt cgggccgcac cacaagaggt gcaagatagg ggggtgcagg cgcgaccata
     3001 cgctctgcgg cggcagagcc tcagcgctgc ctcagtctgc agcgggcagc agaggagtcg
     3061 cgtcgtgcca gagagcgccg ccgtgctcct gagccccttg cgctccgccc ccgcggccca
     3121 ccgacccact gccccttgga tctcgaggga attctaccat ggtgagcaag ggcgaggagg
     3181 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     3241 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     3301 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     3361 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     3421 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     3481 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     3541 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     3601 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     3661 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     3721 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     3781 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     3841 ccggcggcat ggacgagctg tacaagatgc agacaattaa gtgtgttgtt gttggtgatg
     3901 gtgctgttgg taaaacatgt ctcctgatat cctacacaac aaacaaattc ccatcggaat
     3961 atgtaccaac tgtttttgac aactatgcag tcacagttat gattggtgga gagccataca
     4021 ctcttggact ttttgatact gcagggcaag aggattatga cagactacga ccgctaagtt
     4081 atccacagac agatgttttt ctagtatgtt tctcagtggt ctctccatcc tcatttgaaa
     4141 atgtgaaaga aaagtgggtg cctgagataa ctcaccactg tccaaagact cctttcttgc
     4201 ttgttgggac ccaaattgat ctcagagatg acccctctac tattgagaaa cttgccaaga
     4261 acaaacagaa gcctattact ccagagactg ctgaaaagct ggcgcgggat ctgaaggctg
     4321 tcaagtatgt ggagtgctcc gccctcacac agaaaggcct aaagaatgtg tttgatgaag
     4381 caatattggc tgccctggag cctccagaac cgaagaagag ccgcaggtgt gtgctgctat
     4441 gaacgcatct ccagagccct ttctgcacag ctggtgttgt cgtcatacta aaagcaatgt
     4501 ttaaatcaaa ctaaagatta aaaattaaaa tttgtttctg caataatgac aaacgccctg
     4561 cacctaccca catgcactca tgtgagacaa ggcccattag tatggcccct ccttgccctt
     4621 ccagtaatag ttaattttga ataattatgt attgtctgaa aagcaatgag tgctagtttt
     4681 tgttttcttt gtttaaaaaa aaaaacaaaa atccccacaa acgtttgttg tttttttgtt
     4741 taaaagcaag catgcttgta ttgactgtaa cagactaatt cagattgtca aagctgctcc
     4801 ctggttccat tttggagagt aatctggggc atcttcgtgt cttctgtttt gttttgtttt
     4861 gtttttcctc aacccttctc tccttttggg gtgtgtatgg ggttgtttct tagacttgta
     4921 ttttaaaatt tgttaaccat aggacagcct gtggggagga ggacagagta tttccacatt
     4981 ccactcccta ggtctagttt agcaaacgtg tccccacctg gtgctcttag gaaggagtat
     5041 agaaacgcct cgattaacaa cgtattcctt cttgaaagtt gccttttctc tccacctctg
     5101 agtgggtcgg cataatgatg gaactcatga aggtgggtgg acgctgtctc acccctcttt
     5161 ctaggatgca ctctatatgt gactgtgact ttcaagggta tttgtttgcc atttcctgat
     5221 ttagggaggg gaataatttc taacttcttt cattgataaa tgaaaagtat tgcacctttg
     5281 aaatacacca aatggattga gttcctaatt aaaaaaagtt cttttgcccc tgtcagtcat
     5341 tttcttacat gcttagcaca gattcacaac tcactgtgta gtgttcctag attcagccga
     5401 agacctggta cctagggttc ctaacgggat ggtgttagga aacacatcat ggctgatgat
     5461 tgccatctcc tttcaggtga tgaggtcaca ggcctgcttc cttaagaagc tatgagcatg
     5521 gggtggggag catactttta accaacatgg ggaccagtca gggctatccc tttttttctt
     5581 ccatgcctat gaggaacctt tgaacaactg gctgaagctt tcgcaagaaa aatggcgaga
     5641 gcctcatgat tctttataag tgttaataga gactttatca gattattttg tttgcaggtt
     5701 ccagttttta aaaaatgttt aggtaatctc cttccccagt cctgatcttg tggattcgtt
     5761 agtgtcgaac caatgctttc ttgtgtctca actctttgta tatgaattct tttcagatgt
     5821 attaaacaaa caaaaccctt cacaagtgag gtttaaacat cgattgattt acggcgctaa
     5881 ggatgactct ggtcagagat acctggcctg gtctggacac agtgcccgtg tcggagccgc
     5941 gcgagatatg gcccgcgctg gagtttcaat accggagatc atgcaagctg gtggctggac
     6001 caatgtaaat attgtcatga actatatccg taacctggat agtgaaacag gggcaatggt
     6061 gcgcctgctg gaagatggcg atggaccggt gacctagaaa aacatggagc aatcacaagt
     6121 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     6181 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     6241 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     6301 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
     6361 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta
     6421 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta
     6481 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt
     6541 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct
     6601 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     6661 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     6721 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc
     6781 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     6841 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     6901 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     6961 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     7021 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     7081 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     7141 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     7201 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     7261 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     7321 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     7381 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     7441 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     7501 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     7561 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     7621 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     7681 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     7741 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     7801 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     7861 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
     7921 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
     7981 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
     8041 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
     8101 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
     8161 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
     8221 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
     8281 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
     8341 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     8401 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     8461 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8521 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8581 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     8641 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     8701 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     8761 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     8821 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     8881 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8941 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     9001 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     9061 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     9121 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     9181 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     9241 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     9301 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     9361 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     9421 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     9481 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9541 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9601 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9661 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9721 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9781 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9841 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9901 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9961 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    10021 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    10081 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    10141 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    10201 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    10261 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    10321 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    10381 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    10441 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10501 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10561 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10621 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10681 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10741 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
//
