LOCUS       sequence-230551-       10151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of mChe-tagged cdc42E7-E6-3'UTR under pSyn promoter for 
            primary neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10151)
  AUTHORS   Ciolli Mattioli C, Rom A, Franke V, Imami K, Arrey G, Terne M, 
            Woehler A, Akalin A, Ulitsky I, Chekulaeva M
  TITLE     Alternative 3' UTRs direct localization of functionally diverse 
            protein isoforms in neuronal compartments.
  JOURNAL   Nucleic Acids Res. 2018 Dec 22. pii: 5258023. doi: 
            10.1093/nar/gky1270.
  PUBMED    30590745
REFERENCE   2  (bases 1 to 10151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Dec 22. pii: 5258023. doi: 10.1093/nar/gky1270."
COMMENT     Sequence Label: pMCh2008_pLenti_Syn_mChe-cdc42E7-E63'UTR
FEATURES             Location/Qualifiers
     source          1..10151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             3159..3866
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3304..3322)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3572..3591
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             5956..6136
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6162..6179)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6168..6392
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6438..6866
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6525..6544)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6735..6756
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6875..6895)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6880..7209
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7060..7195
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7122..7141
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7257..7304
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7323..7697
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7827..7948
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7864..7883)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7918..7937
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7997..8013)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7997..8013)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8010..8032)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8021..8037
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8045..8051,8052..8069,8070..8075))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8090..8111
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8228..8245)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8399..8987)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8479..8498)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9158..9949,9950..10018))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9781..9800
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10019..10123)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acaagtatct gggaagggta acaatgcaag ttagcctgag
     2641 gatttaggag gaggctgaaa aacagagtag gagccttact acgggtccag accctacgga
     2701 caagaacccc cactcccact ccccaaattg cgcattccct cccccatcag agggggaggg
     2761 gaagaggatg cagcgcggcg cggcgcgtgc gcactgtcgg atttagtacc gcggacagag
     2821 ccttcgcccc cgctgccggc gcgcgccacc acctccccag caccaaaggc gggctgacgt
     2881 cactctccag ccctccccaa actcccctac ctcaccgcct tggtcgcgtc cgtgcagcgg
     2941 tgagtccagt cgggccgcac cacaagaggt gcaagatagg ggggtgcagg cgcgaccata
     3001 cgctctgcgg cggcagagcc tcagcgctgc ctcagtctgc agcgggcagc agaggagtcg
     3061 cgtcgtgcca gagagcgccg ccgtgctcct gagccccttg cgctccgccc ccgcggccca
     3121 ccgacccact gccccttgga tctcgaggga attctaccat ggtgagcaag ggcgaggagg
     3181 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     3241 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     3301 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     3361 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     3421 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     3481 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     3541 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     3601 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     3661 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     3721 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     3781 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     3841 ccggcggcat ggacgagctg tacaagatgc agacaattaa gtgtgttgtt gttggtgatg
     3901 gtgctgttgg taaaacatgt ctcctgatat cctacacaac aaacaaattc ccatcggaat
     3961 atgtaccaac tgtttttgac aactatgcag tcacagttat gattggtgga gagccataca
     4021 ctcttggact ttttgatact gcagggcaag aggattatga cagactacga ccgctaagtt
     4081 atccacagac agatgttttt ctagtatgtt tctcagtggt ctctccatcc tcatttgaaa
     4141 atgtgaaaga aaagtgggtg cctgagataa ctcaccactg tccaaagact cctttcttgc
     4201 ttgttgggac ccaaattgat ctcagagatg acccctctac tattgagaaa cttgccaaga
     4261 acaaacagaa gcctattact ccagagactg ctgaaaagct ggcgcgggat ctgaaggctg
     4321 tcaagtatgt ggagtgctcc gccctcacac agaaaggcct aaagaatgtg tttgatgaag
     4381 caatattggc tgccctggag cctccagaac cgaagaagag ccgcaggtgt gtgctgctat
     4441 gaaccgtttt ctccttcccc tctttgctgc tgcttcctgt cccactactg tagaaagatc
     4501 atttaaaaca aaggaataaa accgtcctgt ttgaaagcct ctgcgtcttc ttactcaaca
     4561 ctttagagca aactctgtat tcatttttga tcatgaatgc aattagaata ttttttgtga
     4621 tcagtagtca cgttagactt gtttaacttt ctgctgcttg agttgcctga tgctcagagc
     4681 ttttggtttg aattattgca aaagggaatg tggtctggca ttaagaatga tgtcttcttg
     4741 gagaaaataa gagttttaac gcttctagat gttagttcta gatggagaaa gcatcactaa
     4801 acatcatttt actcttactg attgttaatt gtaattgcat gacacactat gaaaagggtt
     4861 ggcttgctag tagagtgtaa tggggaaggg aggattctca tggttttcct gtgtgcagtg
     4921 aaacttttgt gttgctccgg taagggctgt ttgtgccgta ggtggagtgt ttgtcattct
     4981 gggatgggga aggtatccat gtatctgctt actgctttat gagttcattg ttatacatta
     5041 tctcctaatc ctagcatcca tttaacaatt gacttaagat ttgctgagat tgaaatgtaa
     5101 agctattgcc tttatatttt cctgcttttg attctattgt tgtgagggaa acatacagtt
     5161 gtggttatct ttacattttg aaattaaaaa tatacaattg tttgtataaa ggtttaaaca
     5221 tcgattgatt tacggcgcta aggatgactc tggtcagaga tacctggcct ggtctggaca
     5281 cagtgcccgt gtcggagccg cgcgagatat ggcccgcgct ggagtttcaa taccggagat
     5341 catgcaagct ggtggctgga ccaatgtaaa tattgtcatg aactatatcc gtaacctgga
     5401 tagtgaaaca ggggcaatgg tgcgcctgct ggaagatggc gatggaccgg tgacctagaa
     5461 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     5521 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     5581 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     5641 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa
     5701 ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc
     5761 tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa
     5821 ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     5881 gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg
     5941 catccggact gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6001 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6061 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6121 tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     6181 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     6241 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     6301 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     6361 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     6421 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     6481 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     6541 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     6601 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     6661 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     6721 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     6781 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     6841 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     6901 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     6961 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7021 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     7081 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     7141 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     7201 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
     7261 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     7321 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
     7381 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
     7441 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
     7501 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
     7561 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
     7621 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
     7681 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
     7741 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     7801 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     7861 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7921 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     7981 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8041 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8101 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8161 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8221 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8281 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8341 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8401 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8461 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8521 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8581 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8641 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8701 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8761 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8821 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8881 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8941 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9001 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9061 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9121 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9181 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9241 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9301 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9361 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9421 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9481 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9541 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9601 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9661 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9721 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9781 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9841 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9901 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9961 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10021 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10081 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10141 tgccacctga c
//
