LOCUS       sequence-230578-        2224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly AB part for pLas-TetO (Promoter activated
            by LasR and repressed by TetR; encodes  logic). Please see 
            Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2224)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P12m
FEATURES             Location/Qualifiers
     source          1..2224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    75..93
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    132..152
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(158..175)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(329..917)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(409..428)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1088..1879,1880..1948))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1711..1730
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1949..2053)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2121..2139
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2143..2186)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2189..2210
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggagctagca agggtccggg ttcaccgaaa tctatctcat ttgctagtta taaaattatg
       61 aaatttgcgt aaattcccta tcagtgatag agattcagaa gcaattacta gagaccatag
      121 tactagagac ctactagtag cggccgctgc aggcttcctc gctcactgac tcgctgcgct
      181 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      241 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      301 accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc
      361 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      421 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      481 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      541 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      601 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      661 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
      721 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
      781 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
      841 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
      901 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
      961 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1021 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1081 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     1141 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     1201 ctggccccag tgctgcaatg ataccgcgcg acccacgctc accggctcca gatttatcag
     1261 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     1321 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     1381 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     1441 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     1501 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     1561 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     1621 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     1681 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     1741 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     1801 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     1861 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     1921 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     1981 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     2041 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     2101 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat
     2161 ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag actagtgggt
     2221 ctca
//
