LOCUS       sequence-230583-        2126 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly AB part for T7 promoter (strong, T7 
            polymerase-dependent constitutive promoter; use with C95m). Please 
            see Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2126)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P18m
FEATURES             Location/Qualifiers
     source          1..2126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    34..54
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(60..77)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(231..819)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(311..330)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(990..1781,1782..1850))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1613..1632
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1851..1955)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2023..2041
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2045..2088)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2091..2112
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggagtaatac gactcactat agtactagag acctactagt agcggccgct gcaggcttcc
       61 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      121 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      181 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg
      241 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      301 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      361 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      421 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      481 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      541 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      601 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      661 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      721 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
      781 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
      841 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
      901 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
      961 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     1021 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1081 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg cgacccacgc
     1141 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1201 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1261 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     1321 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1381 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1441 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1501 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1561 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1621 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1681 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1741 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1801 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1861 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1921 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1981 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     2041 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg
     2101 ccgcttctag agactagtgg gtctca
//
