LOCUS       sequence-23060-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   He TC, Chan TA, Vogelstein B, Kinzler KW
  TITLE     PPARdelta is an APC-regulated target of nonsteroidal 
            anti-inflammatory drugs.
  JOURNAL   Cell. 1999 Oct 29. 99(3):335-45.
  PUBMED    10555149
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1999 
            Oct 29. 99(3):335-45."
COMMENT     Sequence Label: p4xTRE1-Luc
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    243..334
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(275..294)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    348..396
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(664..685)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctttctttat gtttttggcg tcttccatgg tggctttacc aacagtaccg gaatgccaag
       61 ctttacttag atcgcagatc tcgataccgg atccattata tacccgaatt cgatatcgac
      121 gtccagctgt cgagcccggg ctagcgagta caaagacatc gagtacaaag acatgctagc
      181 gagtacaaag acatcgagta caaagacatg ctagcacgcg taagagctcg gtacctatcg
      241 atagagaaat gttctggcac ctgcacttgc actggggaca gcctattttg ctagtttgtt
      301 ttgtttcgtt ttgttttgat ggagagcgta tgttagtact atcgattcac acaaaaaacc
      361 aacacacaga tgtaatgaaa ataaagatat tttattgcgg ccgctccaag tacctcccgt
      421 accttaatat tacttactta tcatggtagc ttgggctggc gtaatagcga agaggcccgc
      481 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcaaat tgtaagcgtt
      541 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
      601 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
      661 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
      721 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagtttttt
//
