LOCUS       sequence-230603-        4808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional unit for constitutive expression of RedF in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Sarrion-Perdigones A, Chang L, Gonzalez Y, Gallego-Flores T, Young 
            DW, Venken KJT
  TITLE     Examining multiple cellular pathways at once using multiplex 
            hextuple luciferase assaying.
  JOURNAL   Nat Commun. 2019 Dec 13;10(1):5710. doi: 10.1038/s41467-019-13651-y.
  PUBMED    31836712
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-12-13"; volume: "10(1):5710. doi"; pages: " 
            10.1038/s41467-019-13651-y"
COMMENT     Sequence Label: hCMV-IE1:RedF
FEATURES             Location/Qualifiers
     source          1..4808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        86..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2274..2498
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2618..3206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2698..2717)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3321..3423
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             3424..4239
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             3424..4239
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3497..3516)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4353..4781
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4440..4459)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4650..4671
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacaagctt cgtctcagtc aggagtagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
      601 tgaaccgtca gatcaagctc aaatggagaa catggagaac gacgagaaca ttgtggtggg
      661 acccaagccg ttctacccga tcgaggaggg ttccgctggc acccagctgc gcaagtacat
      721 ggagcgctac gctaagctgg gtgctatcgc cttcaccaac gccgtgaccg gtgtggatta
      781 ctcctacgcc gagtacctgg agaagtcctg ctgcctcgga aaggccctgc agaactacgg
      841 tctggtcgtg gatggccgca ttgccctctg ctcggagaac tgcgaggagt tcttcattcc
      901 cgtgatcgct ggtctgttca tcggagtcgg cgttgcccca accaacgaga tctacaccct
      961 gcgcgagctg gtccacagcc tgggtatcag caagccgacc atcgttttct cgagcaagaa
     1021 gggcctcgac aaggttatca ccgtgcaaaa aaccgtcacc accatcaaga ccatcgttat
     1081 cctggattcc aaggttgact accgcggtta ccagtgcctc gacaccttca tcaagcgtaa
     1141 caccccaccc ggcttccaag cctcctcctt caaaaccgtg gaggttgatc gcaaggagca
     1201 ggtggccctg atcatgaact cctccggctc caccggtctg ccaaagggcg tgcagctgac
     1261 ccacgagaac accgtcaccc gcttctcgca tgcccgtgat cccatctacg gcaaccaggt
     1321 gtccccaggc accgccgtcc tgaccgtcgt gcccctccac cacggcttcg gcatgttcac
     1381 cactctcggc tacttgatct gcggcttccg tgtggtgatg ctgactaagt tcgacgagga
     1441 gactttcctc aagaccctgc aagactacaa gtgcacttac gtgatcctgg tgccgaccct
     1501 gttcgccatc ctgaacaaga gcgagctgtt gaacaaatac gacctgtcga acctggtgga
     1561 gatcgcttcc ggcggcgccc cactgtcgaa ggaagttggc gaggccgttg ctcgccgctt
     1621 caacctgccc ggcgtccgtc agggttacgg cctgaccgag actacctcgg ctatcatcat
     1681 cacccccgag ggcgacgata agcccggcgc ttccggcaag gttgttccac tcttcaaagc
     1741 caaggtgatc gacctggaca ccaagaagtc cctgggtccc aaccgtcgcg gagaagtgtg
     1801 cgtcaagggc cccatgctga tgaagggcta cgtcaacaac ccagaggcca ccaaggagtt
     1861 gatcgacgag gagggctggc tgcacactgg tgacatcggt tactacgacg aagagaagca
     1921 cttcttcatc gttgaccgcc tgaagtcctt gatcaagtac aagggctacc aggttccccc
     1981 cgccgagctc gagagcgtcc tgctgcagca cccctccatt ttcgatgccg gagtggccgg
     2041 tgtccccgat cccgtggctg gcgagctgcc aggtgccgtc gtcgtgctgg agtccggcaa
     2101 gaacatgacc gagaaggaag tgatggatta cgtggcctcg caagtgtcca acgccaagcg
     2161 cctgcgcggc ggcgtgcgct tcgtggatga ggtgcccaag ggcctcaccg gaaagatcga
     2221 cggtcgcgct atccgcgaga tcctcaagaa gcccgtggcc aagatgtgag cttctgtgcc
     2281 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     2341 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     2401 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     2461 caatagcagg catgctgggg atgcggtggg ctctatggcg cttgagacga agcttgacac
     2521 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     2581 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     2641 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2701 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     2761 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     2821 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     2881 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     2941 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3001 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     3061 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3121 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3181 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3241 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     3301 tcacctagat ccttttgtga tgatcggcac gtaagaggtt ccaactttca ccataatgaa
     3361 ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct
     3421 aaaatgagcc atattcaacg ggaaacgtct tgctcaaggc cgcgattaaa ttccaacatg
     3481 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     3541 atctaccgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     3601 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
     3661 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     3721 gcgatcccag ggaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     3781 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     3841 ccttttaaca gcgatcgcgt atttcgactc gctcaggcgc aatcacgaat gaataacggt
     3901 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     3961 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
     4021 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     4081 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     4141 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     4201 aaattgcagt ttcatttgat gctcgatgag tttttctgat tttttttaag gcagttattg
     4261 gtgcccttaa acgcctggtt gctacgcctg aataagtgat aataagcgga tgaatggcag
     4321 aaattcgatg ataagctgtc aaacatgatg ttacgcgccc tgtagcggcg cattaagcgc
     4381 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4441 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4501 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4561 acttgattag ggtgatggtt cacgtagtgg gccagcgccc tgatagacgg tttttcgccc
     4621 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4681 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     4741 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     4801 tacaattt
//
