LOCUS       sequence-23061-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   He TC, Chan TA, Vogelstein B, Kinzler KW
  TITLE     PPARdelta is an APC-regulated target of nonsteroidal 
            anti-inflammatory drugs.
  JOURNAL   Cell. 1999 Oct 29. 99(3):335-45.
  PUBMED    10555149
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1999 
            Oct 29. 99(3):335-45."
COMMENT     Sequence Label: p4xTRE2-Luc
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    246..337
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(278..297)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    351..399
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(667..688)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     879..898
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcctttcttt atgtttttgg cgtcttccat ggtggcttta ccaacagtac cggaatgcca
       61 agcttactta gatcgcagat ctcgataccg gatccattat atacccgaat tcgatatcga
      121 cgtccagctg tcgagcccgg gctagctcag atgaaagcca atcagatgaa agccaagcta
      181 gctcagatga aagccaatca gatgaaagcc aagctagcac gcgtaagagc tcggtaccta
      241 tcgatagaga aatgttctgg cacctgcact tgcactgggg acagcctatt ttgctagttt
      301 gttttgtttc gttttgtttt gatggagagc gtatgttagt actatcgatt cacacaaaaa
      361 accaacacac agatgtaatg aaaataaaga tattttattg cggccgctcc aagtacctcc
      421 cgtaccttaa tattacttac ttatcatggt agcttgggct ggcgtaatag cgaagaggcc
      481 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggca aattgtaagc
      541 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
      601 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
      661 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
      721 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
      781 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
      841 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
      901 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg ta
//
