LOCUS       sequence-230612-        2239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly AB for pSalTTTC (Adam Meyer's improved 
            pSal promoter from https://doi.org/10.1101/285866, selected for 
            dynamic range and less cross-sensitivity)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2239)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P60m
FEATURES             Location/Qualifiers
     source          1..2239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..86
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(90..133)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    136..157
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    318..338
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(344..361)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(515..1103)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(595..614)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1274..2065,2066..2134))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1897..1916
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2135..2239)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
       61 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
      121 taaggatgat ttctggaatt cgcggccgct tctagagact agtgggtctc aggagaacga
      181 tcgttggctg ggggcctcgc ttgggttatt gctggtgccc ggccgggcgc aatattcatg
      241 ttgatgattt attatatatc gagtggtgta tttatttata ttgtttgctc cgttaccgtt
      301 attaactact agagacctac tagtagcggc cgctgcaggc ttcctcgctc actgactcgc
      361 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      421 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
      481 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg
      541 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      601 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      661 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      721 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      781 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      841 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      901 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      961 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1021 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1081 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1141 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1201 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1261 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     1321 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     1381 taccatctgg ccccagtgct gcaatgatac cgcgcgaccc acgctcaccg gctccagatt
     1441 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1501 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1561 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1621 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1681 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1741 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1801 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1861 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1921 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1981 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     2041 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     2101 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     2161 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     2221 aacaaatagg ggttccgcg
//
