LOCUS       sequence-230618-        2825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for sfGFP (Superfolder green 
            fluorescent protein from DOI: 10.1038/nbt1172; slight variant on 
            https://www.fpbase.org/protein/superfolder-gfp/).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2825)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C3m
FEATURES             Location/Qualifiers
     source          1..2825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(631..1422,1423..1491))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1254..1273
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1492..1596)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1664..1682
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1686..1729)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1732..1753
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             1769..2482
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    2500..2520
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(2526..2543)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2697..2825,1..460))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2777..2796)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
       61 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      121 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
      181 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
      241 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
      301 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
      361 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
      421 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
      481 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
      541 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
      601 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
      661 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
      721 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gcgacccacg
      781 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
      841 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
      901 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
      961 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     1021 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     1081 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     1141 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     1201 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     1261 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     1321 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     1381 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     1441 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     1501 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     1561 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     1621 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     1681 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcgcg
     1741 gccgcttcta gagactagtg ggtctcaaat gcgtaaaggc gaagagctgt tcactggtgt
     1801 cgtccctatt ctggtggaac tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg
     1861 cgagggtgaa ggtgacgcaa ctaatggtaa actgacgctg aagttcatct gtactactgg
     1921 taaactgccg gtaccttggc cgactctggt aacgacgctg acttatggtg ttcagtgctt
     1981 tgctcgttat ccggaccata tgaagcagca tgacttcttc aagtccgcca tgccggaagg
     2041 ctatgtgcag gaacgcacga tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga
     2101 agtgaaattt gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa
     2161 agaagatggc aatatcctgg gccataagct ggaatacaat tttaacagcc acaatgttta
     2221 catcaccgcc gataaacaaa aaaatggcat taaagcgaat tttaaaattc gccacaacgt
     2281 ggaggatggc agcgtgcagc tggctgatca ctaccagcaa aacactccaa tcggtgatgg
     2341 tcctgttctg ctgccagaca atcactatct gagcacgcaa agcgttctgt ctaaagatcc
     2401 gaacgagaaa cgcgatcata tggttctgct ggagttcgta accgcagcgg gcatcacgca
     2461 tggtatggat gaactgtaca aatgatgaag gtagagacct actagtagcg gccgctgcag
     2521 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     2581 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     2641 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     2701 cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     2761 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     2821 cctgt
//
