LOCUS       sequence-230653-        9677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoter expression plasmid for human codon optimized LbBE1.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9677)
  AUTHORS   Kleinstiver BP, Sousa AA, Walton RT, Tak YE, Hsu JY, Clement K, 
            Welch MM, Horng JE, Malagon-Lopez J, Scarfo I, Maus MV, Pinello L, 
            Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas12a variants with increased activities and 
            improved targeting ranges for gene, epigenetic and base editing.
  JOURNAL   Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0.
  PUBMED    30742127
REFERENCE   2  (bases 1 to 9677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0."
COMMENT     Sequence Label: 
            pCAG-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-NLS(nucleoplasmin)-gs-UGI
            -NLS(SV40)(LbBE1.1) (RTW1295)
FEATURES             Location/Qualifiers
     source          1..9677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          348..1364
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1288..1310
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1372..1391
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1457..1466
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1463..2152
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MGSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINW
                     GGRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEF
                     LSRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPS
                     NEAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPH
                     ILWATGLK"
     CDS             2249..5929
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     CDS             5930..5977
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6014..6262
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6275..6295
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6385..6404)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6450..6505
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6504..6523)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6866..6882)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6866..6882)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6879..6901)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6890..6906
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6914..6920,6921..6938,6939..6944))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6959..6980
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7039..7234
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7085..7220
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7240..7374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7277..7296)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7331..7350
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7442..7459)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7613..8201)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7693..7712)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8372..9163,9164..9232))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8995..9014
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9233..9337)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        join(9368..9677,1..70)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
       61 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
      121 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
      181 gggggggggg gggcgcgccs gggggggggg gggggggggg gggggggggg gggggggggg
      241 gggggggggg gggggcrgcc aaycagrgsg gcgcgcyccg aaagtttcct tttwtggsgr
      301 ggsggsggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggsggga gtcgctgcgc
      361 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
      421 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
      481 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
      541 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
      601 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
      661 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
      721 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
      781 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
      841 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
      901 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgccycggg
      961 ccgggggggg ctcgggggrg gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     1021 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     1081 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     1141 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     1201 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     1261 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     1321 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     1381 tggttattgt gctgtctcat cattttggca aagaattctg cagtcgacgg taccgcgggc
     1441 ccgggatcca ccggtcgcca ccatgggcag ctcagagact ggcccagtgg ctgtggaccc
     1501 cacattgaga cggcggatcg agccccatga gtttgaggta ttcttcgatc cgagagagct
     1561 ccgcaaggag acctgcctgc tttacgaaat taattggggg ggccggcact ccatttggcg
     1621 acatacatca cagaacacta acaagcacgt cgaagtcaac ttcatcgaga agttcacgac
     1681 agaaagatat ttctgtccga acacaaggtg cagcattacc tggtttctca gctggagccc
     1741 atgcggcgaa tgtagtaggg ccatcactga attcctgtca aggtatcccc acgtcactct
     1801 gtttatttac atcgcaaggc tgtaccacca cgctgacccc cgcaatcgac aaggcctgcg
     1861 ggatttgatc tcttcaggtg tgactatcca aattatgact gagcaggagt caggatactg
     1921 ctggagaaac tttgtgaatt atagcccgag taatgaagcc cactggccta ggtatcccca
     1981 tctgtgggta cgactgtacg ttcttgaact gtactgcatc atactgggcc tgcctccttg
     2041 tctcaacatt ctgagaagga agcagccaca gctgacattc tttaccatcg ctcttcagtc
     2101 ttgtcattac cagcgactgc ccccacacat tctctgggcc accgggttga aatctggtgg
     2161 ttcttctggt ggttctagcg gcagcgagac tcccgggacc tcagagtccg ccacacccga
     2221 aagttccgga gggagtagcg gcgggtctag caagctggag aagtttacaa actgctactc
     2281 cctgtctaag accctgaggt tcaaggccat ccctgtgggc aagacccagg agaacatcga
     2341 caataagcgg ctgctggtgg aggacgagaa gagagccgag gattataagg gcgtgaagaa
     2401 gctgctggat cgctactatc tgtcttttat caacgacgtg ctgcacagca tcaagctgaa
     2461 gaatctgaac aattacatca gcctgttccg gaagaaaacc agaaccgaga aggagaataa
     2521 ggagctggag aacctggaga tcaatctgcg gaaggagatc gccaaggcct tcaagggcaa
     2581 cgagggctac aagtccctgt ttaagaagga tatcatcgag acaatcctgc cagagttcct
     2641 ggacgataag gacgagatcg ccctggtgaa cagcttcaat ggctttacca cagccttcac
     2701 cggcttcttt gataacagag agaatatgtt ttccgaggag gccaagagca catccatcgc
     2761 cttcaggtgt atcaacgaga atctgacccg ctacatctct aatatggaca tcttcgagaa
     2821 ggtggacgcc atctttgata agcacgaggt gcaggagatc aaggagaaga tcctgaacag
     2881 cgactatgat gtggaggatt tctttgaggg cgagttcttt aactttgtgc tgacacagga
     2941 gggcatcgac gtgtataacg ccatcatcgg cggcttcgtg accgagagcg gcgagaagat
     3001 caagggcctg aacgagtaca tcaacctgta taatcagaaa accaagcaga agctgcctaa
     3061 gtttaagcca ctgtataagc aggtgctgag cgatcgggag tctctgagct tctacggcga
     3121 gggctataca tccgatgagg aggtgctgga ggtgtttaga aacaccctga acaagaacag
     3181 cgagatcttc agctccatca agaagctgga gaagctgttc aagaattttg acgagtactc
     3241 tagcgccggc atctttgtga agaacggccc cgccatcagc acaatctcca aggatatctt
     3301 cggcgagtgg aacgtgatcc gggacaagtg gaatgccgag tatgacgata tccacctgaa
     3361 gaagaaggcc gtggtgaccg agaagtacga ggacgatcgg agaaagtcct tcaagaagat
     3421 cggctccttt tctctggagc agctgcagga gtacgccgac gccgatctgt ctgtggtgga
     3481 gaagctgaag gagatcatca tccagaaggt ggatgagatc tacaaggtgt atggctcctc
     3541 tgagaagctg ttcgacgccg attttgtgct ggagaagagc ctgaagaaga acgacgccgt
     3601 ggtggccatc atgaaggacc tgctggattc tgtgaagagc ttcgagaatt acatcaaggc
     3661 cttctttggc gagggcaagg agacaaacag ggacgagtcc ttctatggcg attttgtgct
     3721 ggcctacgac atcctgctga aggtggacca catctacgat gccatccgca attatgtgac
     3781 ccagaagccc tactctaagg ataagttcaa gctgtatttt cagaaccctc agttcatggg
     3841 cggctgggac aaggataagg agacagacta tcgggccacc atcctgagat acggctccaa
     3901 gtactatctg gccatcatgg ataagaagta cgccaagtgc ctgcagaaga tcgacaagga
     3961 cgatgtgaac ggcaattacg agaagatcaa ctataagctg ctgcccggcc ctaataagat
     4021 gctgccaaag gtgttctttt ctaagaagtg gatggcctac tataacccca gcgaggacat
     4081 ccagaagatc tacaagaatg gcacattcaa gaagggcgat atgtttaacc tgaatgactg
     4141 tcacaagctg atcgacttct ttaaggatag catctcccgg tatccaaagt ggtccaatgc
     4201 ctacgatttc aacttttctg agacagagaa gtataaggac atcgccggct tttacagaga
     4261 ggtggaggag cagggctata aggtgagctt cgagtctgcc agcaagaagg aggtggataa
     4321 gctggtggag gagggcaagc tgtatatgtt ccagatctat aacaaggact tttccgataa
     4381 gtctcacggc acacccaatc tgcacaccat gtacttcaag ctgctgtttg acgagaacaa
     4441 tcacggacag atcaggctga gcggaggagc agagctgttc atgaggcgcg cctccctgaa
     4501 gaaggaggag ctggtggtgc acccagccaa ctcccctatc gccaacaaga atccagataa
     4561 tcccaagaaa accacaaccc tgtcctacga cgtgtataag gataagaggt tttctgagga
     4621 ccagtacgag ctgcacatcc caatcgccat caataagtgc cccaagaaca tcttcaagat
     4681 caatacagag gtgcgcgtgc tgctgaagca cgacgataac ccctatgtga tcggcatcgc
     4741 caggggcgag cgcaatctgc tgtatatcgt ggtggtggac ggcaagggca acatcgtgga
     4801 gcagtattcc ctgaacgaga tcatcaacaa cttcaacggc atcaggatca agacagatta
     4861 ccactctctg ctggacaaga aggagaagga gaggttcgag gcccgccaga actggacctc
     4921 catcgagaat atcaaggagc tgaaggccgg ctatatctct caggtggtgc acaagatctg
     4981 cgagctggtg gagaagtacg atgccgtgat cgccctggag gacctgaact ctggctttaa
     5041 gaatagccgc gtgaaggtgg agaagcaggt gtatcagaag ttcgagaaga tgctgatcga
     5101 taagctgaac tacatggtgg acaagaagtc taatccttgt gcaacaggcg gcgccctgaa
     5161 gggctatcag atcaccaata agttcgagag ctttaagtcc atgtctaccc agaacggctt
     5221 catcttttac atccctgcct ggctgacatc caagatcgat ccatctaccg gctttgtgaa
     5281 cctgctgaaa accaagtata ccagcatcgc cgattccaag aagttcatca gctcctttga
     5341 caggatcatg tacgtgcccg aggaggatct gttcgagttt gccctggact ataagaactt
     5401 ctctcgcaca gacgccgatt acatcaagaa gtggaagctg tactcctacg gcaaccggat
     5461 cagaatcttc cggaatccta agaagaacaa cgtgttcgac tgggaggagg tgtgcctgac
     5521 cagcgcctat aaggagctgt tcaacaagta cggcatcaat tatcagcagg gcgatatcag
     5581 agccctgctg tgcgagcagt ccgacaaggc cttctactct agctttatgg ccctgatgag
     5641 cctgatgctg cagatgcgga acagcatcac aggccgcacc gacgtggatt ttctgatcag
     5701 ccctgtgaag aactccgacg gcatcttcta cgatagccgg aactatgagg cccaggagaa
     5761 tgccatcctg ccaaagaacg ccgacgccaa tggcgcctat aacatcgcca gaaaggtgct
     5821 gtgggccatc ggccagttca agaaggccga ggacgagaag ctggataagg tgaagatcgc
     5881 catctctaac aaggagtggc tggagtacgc ccagaccagc gtgaagcaca aaaggccggc
     5941 ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggga tcctctggtg gttctggagg
     6001 atctggtggt tctactaatc tgtcagatat tattgaaaag gagaccggta agcaactggt
     6061 tatccaggaa tccatcctca tgctcccaga ggaggtggaa gaagtcattg ggaacaagcc
     6121 ggaaagcgat atactcgtgc acaccgccta cgacgagagc accgacgaga atgtcatgct
     6181 tctgactagc gacgcccctg aatacaagcc ttgggctctg gtcatacagg atagcaacgg
     6241 tgagaacaag attaagatgc tctctggtgg ttctcccaag aagaagagga aagtctaaag
     6301 cggccgcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     6361 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     6421 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     6481 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     6541 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     6601 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     6661 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     6721 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     6781 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
     6841 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6901 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6961 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7021 tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     7081 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7141 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     7201 aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat
     7261 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7321 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc
     7381 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7441 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7501 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7561 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7621 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7681 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7741 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7801 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7861 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7921 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7981 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8041 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8101 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8161 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8221 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8281 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8341 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8401 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8461 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8521 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8581 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8641 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8701 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8761 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8821 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8881 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8941 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9001 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9061 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9121 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9181 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9241 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9301 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9361 ctgggtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     9421 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     9481 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     9541 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     9601 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     9661 cccgcctggc attatgc
//
