LOCUS       sequence-23068-e         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Yu J, Zhang L, Hwang PM, Rago C, Kinzler KW, Vogelstein B
  TITLE     Identification and classification of p53-regulated genes.
  JOURNAL   Proc Natl Acad Sci U S A. 1999 Dec 7. 96(25):14517-22.
  PUBMED    10588737
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1999 Dec 7. 96(25):14517-22."
COMMENT     Sequence Label: tTA-IRES-Neo
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    87..639
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(249..268)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     478..495
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcagatc
       61 cgaaaatgga tatccaagct cccccatatt atcatcgtgt ttttcaaagg aaaaccacgt
      121 ccccgtggtt cggggggcct agacgttttt ttaacctcga ctaaacacat gtaaagcatg
      181 tgcaccgagg ccccagatca gatcccatac aatggggtac cttctgggca tccttcagcc
      241 ccttgttgaa tacgcttgag gagagccatt tgactctttc cacaactatc caactcacaa
      301 cgtggcactg gggttgtgcc gcctttgcag gtgtatctta tacacgtggc ttttggccgc
      361 agaggcacct gtcgccaggt ggggggttcc gctgcctgca aagggtcgct acagacgttg
      421 tttgtcttca agaagcttcc agaggaactg cttccttcac gacattcaac agaccttgca
      481 ttcctttggc gagaggggaa agacccctag gaatgctcgt caagaagaca gggccaggtt
      541 tccgggccct cacattgcca aaagacggca atatggtgga aaataacata tagacaaacg
      601 cacaccggcc ttattccaag cggcttcggc cagtaacgtt aggggggggg gggggg
//
