LOCUS       sequence-23082-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Hermeking H, Rago C, Schuhmacher M, Li Q, Barrett JF, Obaya AJ, 
            O'Connell BC, Mateyak MK, Tam W, Kohlhuber F, Dang CV, Sedivy JM, 
            Eick D, Vogelstein B, Kinzler KW
  TITLE     Identification of CDK4 as a target of c-MYC.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 Feb 29. 97(5):2229-34.
  PUBMED    10688915
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2000 Feb 29. 97(5):2229-34."
COMMENT     Sequence Label: MadMyc-HA
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     136..155
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        136..154
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      170..179
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             460..486
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             731..760
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cgtggggntt tagcagagct cgtttgtgaa ccgtcagatc gcctggagac gccatccacg
       61 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc ccgaattcga
      121 gctcggtacg cgcgctaata cgactcacta tagggagagg taccttccac catggcggcg
      181 gcggttcgga tgaacatcca gatgctgctg gaggcggccg actatctgga gcggcgggag
      241 agagaagctg aacatggtta tgcctccatg ttaccataca ataacatcga tgttgtttct
      301 gtggaaaaga ggcaggctcc tggcaaaagg tcagagtctg gatcaccttc tgctggaggc
      361 cacagcaaac ctcctcacag cccactggtc ctcaagaggt gccacgtctc cacacatcag
      421 cacaactacg cagcgcctcc ctccactcgg aaggactatc ctgctgccaa gagggtcaag
      481 ttggacagtg tcagagtcct gagacagatc agcaacaacc gaaaatgcac cagccccagg
      541 tcctcggaca ccgaggagaa tgtcaagagg cgaacacaca acgtcttgga gcgccagagg
      601 aggaacgagc taaaacggag cttttttgcc ctgcgtgacc agatcccgga gttgaaanga
      661 gcagaanncc cccccanggc gntttatcct taaaaaagcc acagcataca tcctgtccgt
      721 ccaagcagag gagcaaaagc tcatttctga agaggacttg ttgcggaacg acgagnacag
      781 ttgaaacaca acttgacagc tacggactct tgtgcgtacc tttgatgtgc agatatgcng
      841 gannc
//
