LOCUS       sequence-23085-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Hermeking H, Rago C, Schuhmacher M, Li Q, Barrett JF, Obaya AJ, 
            O'Connell BC, Mateyak MK, Tam W, Kohlhuber F, Dang CV, Sedivy JM, 
            Eick D, Vogelstein B, Kinzler KW
  TITLE     Identification of CDK4 as a target of c-MYC.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 Feb 29. 97(5):2229-34.
  PUBMED    10688915
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2000 Feb 29. 97(5):2229-34."
COMMENT     Sequence Label: pBV-Luc mut MBS1
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    353..444
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(385..404)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    458..506
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 cttcgcggat ganggcgccg ggcctttctt tatgtttttg gcgtcttcca tggtggcttt
       61 accaacagta ccggaatgcc aagcttactt agatcgcaga tctcgatacc ggatccatta
      121 tatacccgaa ttccggacgt tctgggcacg tgaccgccac ccatgcgctg aggggcggac
      181 aggacgtgct tcgacttggg aggagggcga agagtgtaag gggacggagg ggcgatggca
      241 gccacgtgat ctgttgctat cacacgtgac cgcttgacat tgctctgagg agcagaggtg
      301 tggctacgcg gcgggcaccg tgactggctg ccacaacccg gtacctatcg atagagaaat
      361 gttctggcac ctgcacttgc actggggaca gcctattttg ctagtttgtt ttgtttcgtt
      421 ttgttttgat ggagagcgta tgttagtact atcgattcac acaaaaaacc aacacacaga
      481 tgtaatgaaa ataaagatat tttattgcgg ccgctccaag tacctcccgt accttaatat
      541 tacttactta tcatggtagc ttgggctggc gtaatagcga agaggcccgc accgatcgcc
      601 cttcccaaca gttgcgcagc ctgaatggcg aatggcaaat tgtaagcgtt aatattttgt
      661 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
      721 gcaaaatccc ttataaatca aaaaaaaccc cgaaaaggtg agttgntttt ccanttgaaa
      781 aaaagtcact attaaagacg tggactccac gtcaanggcg aaaaccgcta tcaggcgatg
      841 nccctacgtg aacatc
//
