LOCUS       sequence-23086-e         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Hermeking H, Rago C, Schuhmacher M, Li Q, Barrett JF, Obaya AJ, 
            O'Connell BC, Mateyak MK, Tam W, Kohlhuber F, Dang CV, Sedivy JM, 
            Eick D, Vogelstein B, Kinzler KW
  TITLE     Identification of CDK4 as a target of c-MYC.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 Feb 29. 97(5):2229-34.
  PUBMED    10688915
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2000 Feb 29. 97(5):2229-34."
COMMENT     Sequence Label: pBV-Luc mut MBS2
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      47..56
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    351..442
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(383..402)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    456..504
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 tcgcggaang atggcgccgg gcctttcttt atgtttttgg cgtcttccat ggtggcttta
       61 ccaacagtac cggaatgcca agcttactta gatcgcagat ctcgataccg gatccattat
      121 atacccgaat tccggacgtt ctgggcacgt gaccgccacc catgcgctga ggggcggaca
      181 ggaggtgctt cgactgggag gagggcgaag agtgtaaggg ggcggagggg cgatggccgc
      241 cacgtggatc tgttgcctat cacacgggac cgcttgacat tgctctgagg agcagaggtg
      301 tggctacgcg gcgggcacgt gactggctgc cacacccggt acctatcgat agagaaatgt
      361 tctggcacct gcacttgcac tggggacagc ctattttgct agtttgtttt gtttcgtttt
      421 gttttgatgg agagcgtatg ttagtactat cgattcacac aaaaaaccaa cacacagatg
      481 taatgaaaat aaagatattt tattgcggcc gctccaagta cctcccgtac cttaatatta
      541 cttacttatc atggtagctt gggctggcgt aatagcgaag aggcccgcac cgatcgccct
      601 tcccaacagt tgcgcagcct gaatggcgaa tggcaaattg taagcgttaa tattttgtta
      661 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
      721 aaatccctta taatcaaaag atagaccntg ataggntgat gttgttcnnt ttgaacagag
      781 tcactattaa aaacgtgant ccacgtcaag gcgaaaccgt ctatcaggca ngnccctact
      841 gaac
//
