LOCUS       sequence-23087-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Hermeking H, Rago C, Schuhmacher M, Li Q, Barrett JF, Obaya AJ, 
            O'Connell BC, Mateyak MK, Tam W, Kohlhuber F, Dang CV, Sedivy JM, 
            Eick D, Vogelstein B, Kinzler KW
  TITLE     Identification of CDK4 as a target of c-MYC.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 Feb 29. 97(5):2229-34.
  PUBMED    10688915
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2000 Feb 29. 97(5):2229-34."
COMMENT     Sequence Label: pBV-Luc mut MBS1-3
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      49..58
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    351..442
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(383..402)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    456..504
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 cnttcgcgga nganggcgcc gggcctttct ttatgttttt ggcgtcttcc atggtggctt
       61 taccaacagt accggaatgc caagcttact tagatcgcag atctcgatac cggatccatt
      121 atatacccga attccggacg ttctgggcac gtgaccgcca cccatgcgct gaggggcgga
      181 caggagtgct tcgactggga ggagggcgaa gagtgtaagg gggcggaggg gcgatggcag
      241 ccaggtgatc tgttgctatc acaggtgacc gcttgacatt gctctgagga gcagaggtgt
      301 ggctacgcgg cgggcaggtg actggctgcc acaacccggt acctatcgat agagaaatgt
      361 tctggcacct gcacttgcac tggggacagc ctattttgct agtttgtttt gtttcgtttt
      421 gttttgatgg agagcgtatg ttagtactat cgattcacac aaaaaaccaa cacacagatg
      481 taatgaaaat aaagatattt tattgcggcc gctccaagta cctcccgtac cttaatatta
      541 cttacttatc atggtagctt gggctggcgt aatagcgaag aggcccgcac cgatcgccct
      601 tcccaacagt tgcgcagcct gaatggcgaa tggcaaattg taagcgttaa tattttgtta
      661 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc nnaatcggca
      721 aaatccctta taatcaaaga atagaccgag atagggttga gtgttgtncc agttggaaca
      781 gagtcactat taagaacgtg gactcnacgt caaaggcgaa accgtct
//
