LOCUS       sequence-23089-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Hermeking H, Rago C, Schuhmacher M, Li Q, Barrett JF, Obaya AJ, 
            O'Connell BC, Mateyak MK, Tam W, Kohlhuber F, Dang CV, Sedivy JM, 
            Eick D, Vogelstein B, Kinzler KW
  TITLE     Identification of CDK4 as a target of c-MYC.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 Feb 29. 97(5):2229-34.
  PUBMED    10688915
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2000 Feb 29. 97(5):2229-34."
COMMENT     Sequence Label: pBV-Luc mut MBS4
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      51..60
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    358..449
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(390..409)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    463..511
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 ctttcncggn angaatggcg ccgggccttt ctttatgttt ttggcgtctt ccatggtggc
       61 tttaccaaca gtaccggaat gccaagctta cttagatcgc agatctcgat accggatcca
      121 ttatataccc gaattccggg gacggttctg ggcaggtgac cgcccaccca tgcgctgagg
      181 ggcggacagg aggtgcttcg actgggagga gggcgaagag tgtaaggggg cggaggggcg
      241 atggcagcca cgtgatcggt tgctatcaca cgtgaccgct tgacattgct ctgaggagca
      301 gaggtgtggc tacgcggcgg gcacgtgact ggctgccaca acccggtacc tatcgataga
      361 gaaatgttct ggcacctgca cttgcactgg ggacagccta ttttgctagt ttgttttgtt
      421 tcgttttgtt ttgatggaga gcgtatgtta gtactatcga ttcacacaaa aaaccaacac
      481 acagatgtaa tgaaaataaa gatattttat tgcggccgct ccaagtacct cccgtacctt
      541 aatattactt acttatcatg gtagcttggg ctggcgtaat agcgaagagg cccgcaccga
      601 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg caaattgtaa gcgttaatat
      661 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
      721 aatcggcaaa atcctnataa tcaaaaatag accgagatag gttgagtgtt gtccagtttg
      781 gacaagagtc nctnatnaaa aacgtggact ccacgtcaaa ggcgaaaccg t
//
