LOCUS       sequence-23094-e         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Shih IM, Yu J, He TC, Vogelstein B, Kinzler KW
  TITLE     The beta-catenin binding domain of adenomatous polyposis coli is 
            sufficient for tumor suppression.
  JOURNAL   Cancer Res. 2000 Mar 15. 60(6):1671-6.
  PUBMED    10749138
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2000 Mar 15. 60(6):1671-6."
COMMENT     Sequence Label: pGL3-OF (mutant TCF-4 sites)
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    240..331
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(272..291)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    345..393
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(661..682)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tcgcggaaga tggcgccggg cctttcttta tgtttttggc gtcttccatg gtggctttac
       61 caacagtacc ggaatgccaa gcttacttag atcgcagatc tcgagtaccg gatccattat
      121 ataccctcta gacctttggc cttaccccct ttggccttac cccctttggc cctgcaggca
      181 tgcaagcttc agctgctcga gcccgggcta gcacgcgtaa gagctcggta cctatcgata
      241 gagaaatgtt ctggcacctg cacttgcact ggggacagcc tattttgcta gtttgttttg
      301 tttcgttttg ttttgatgga gagcgtatgt tagtactatc gattcacaca aaaaaccaac
      361 acacagatgt aatgaaaata aagatatttt attgcggccg ctccaagtac ctcccgtacc
      421 ttaatattac ttacttatca tggtagcttg ggctggcgta atagcgaaga ggcccgcacc
      481 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcaaattgt aagcgttaat
      541 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
      601 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
      661 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaac
//
