LOCUS       sequence-23096-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Shih IM, Torrance C, Sokoll LJ, Chan DW, Kinzler KW, Vogelstein B
  TITLE     Assessing tumors in living animals through measurement of urinary 
            beta-human chorionic gonadotropin.
  JOURNAL   Nat Med. 2000 Jun . 6(6):711-4.
  PUBMED    10835692
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med. 
            2000 Jun . 6(6):711-4."
COMMENT     Sequence Label: pCI-neo-hCG
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    606..727
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(617..636)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctagtagctt ggcattccgg tactgttggt aaagccacca tgggcatgga gatgttccag
       61 gggctgctgc tgttgctgct gctgagcatg ggcgggacat gggcatccaa ggagccgctt
      121 cggccacggt gccgccccat caatgccacc ctggctgtgg agaaggaggg ctgccccgtg
      181 tgcatcaccg tcaacaccac catctgtgcc ggctactgcc ccaccatgac ccgcgtgctg
      241 cagggggtcc tgccggccct gcctcaggtg gtgtgcaact accgcgatgt gcgcttcgag
      301 tccatccggc tccctggctg cccgcgcggc gtgaaccccg tggtctccta cgccgtggct
      361 ctcagctgtc aatgtgcact ctgccgccgc agcaccactg actgcggggg tcccaaggac
      421 caccccttga cctgtgatga cccccgcttc caggactcct cttcctcaaa ggcccctccc
      481 cccagccttc caagtccatc ccgactcccg gggccctcgg acaccccgat cctcccacaa
      541 taatctagag tcgacccggg cggccgcttc cctttagtga gggttaatgc ttcgagcaga
      601 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
      661 ctttatttgt gaaatttgtg atgctattgt tttatttgta accattataa gctgcangaa
      721 acaagttaac aacaacaatt gtttttnttt tattattcng gtggggggga antntttttt
      781 ttttttaaaa anaaaaaacc ttnaaatggg aaaaa
//
