LOCUS       sequence-231032-        9914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoter expression plasmid for human codon optimized AsBE1.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9914)
  AUTHORS   Kleinstiver BP, Sousa AA, Walton RT, Tak YE, Hsu JY, Clement K, 
            Welch MM, Horng JE, Malagon-Lopez J, Scarfo I, Maus MV, Pinello L, 
            Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas12a variants with increased activities and 
            improved targeting ranges for gene, epigenetic and base editing.
  JOURNAL   Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0.
  PUBMED    30742127
REFERENCE   2  (bases 1 to 9914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0."
COMMENT     Sequence Label: 
            pCAG-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-UGI
            -NLS(SV40)(AsBE1.1) (RTW1351)
FEATURES             Location/Qualifiers
     source          1..9914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          160..1176
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1100..1122
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1184..1203
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1269..1278
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1275..1964
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MGSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINW
                     GGRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEF
                     LSRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPS
                     NEAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPH
                     ILWATGLK"
     CDS             2061..5978
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     CDS             5979..6026
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6063..6311
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6324..6344
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6434..6453)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6499..6554
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6553..6572)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6915..6931)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6915..6931)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6928..6950)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6939..6955
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6963..6969,6970..6987,6988..6993))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7008..7029
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7088..7283
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7134..7269
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7289..7423
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7326..7345)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7380..7399
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7491..7508)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7662..8250)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7742..7761)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8421..9212,9213..9281))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9044..9063
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9282..9386)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        9417..9796
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggsgcgcgc crggsggggs ggggsggggs grgggggggg gsggggsgrg gcggrgrggt
       61 gsggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
      121 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
      181 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
      241 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
      301 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
      361 ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
      421 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
      481 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
      541 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
      601 ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag
      661 cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg
      721 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
      781 ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
      841 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
      901 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
      961 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     1021 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
     1081 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
     1141 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
     1201 gtgctgtctc atcattttgg caaagaattc tgcagtcgac ggtaccgcgg gcccgggatc
     1261 caccggtcgc caccatgggc agctcagaga ctggcccagt ggctgtggac cccacattga
     1321 gacggcggat cgagccccat gagtttgagg tattcttcga tccgagagag ctccgcaagg
     1381 agacctgcct gctttacgaa attaattggg ggggccggca ctccatttgg cgacatacat
     1441 cacagaacac taacaagcac gtcgaagtca acttcatcga gaagttcacg acagaaagat
     1501 atttctgtcc gaacacaagg tgcagcatta cctggtttct cagctggagc ccatgcggcg
     1561 aatgtagtag ggccatcact gaattcctgt caaggtatcc ccacgtcact ctgtttattt
     1621 acatcgcaag gctgtaccac cacgctgacc cccgcaatcg acaaggcctg cgggatttga
     1681 tctcttcagg tgtgactatc caaattatga ctgagcagga gtcaggatac tgctggagaa
     1741 actttgtgaa ttatagcccg agtaatgaag cccactggcc taggtatccc catctgtggg
     1801 tacgactgta cgttcttgaa ctgtactgca tcatactggg cctgcctcct tgtctcaaca
     1861 ttctgagaag gaagcagcca cagctgacat tctttaccat cgctcttcag tcttgtcatt
     1921 accagcgact gcccccacac attctctggg ccaccgggtt gaaatctggt ggttcttctg
     1981 gtggttctag cggcagcgag actcccggga cctcagagtc cgccacaccc gaaagttccg
     2041 gagggagtag cggcgggtct acacagttcg agggctttac caacctgtat caggtgagca
     2101 agacactgcg gtttgagctg atcccacagg gcaagaccct gaagcacatc caggagcagg
     2161 gcttcatcga ggaggacaag gcccgcaatg atcactacaa ggagctgaag cccatcatcg
     2221 atcggatcta caagacctat gccgaccagt gcctgcagct ggtgcagctg gattgggaga
     2281 acctgagcgc cgccatcgac tcctatagaa aggagaaaac cgaggagaca aggaacgccc
     2341 tgatcgagga gcaggccaca tatcgcaatg ccatccacga ctacttcatc ggccggacag
     2401 acaacctgac cgatgccatc aataagagac acgccgagat ctacaagggc ctgttcaagg
     2461 ccgagctgtt taatggcaag gtgctgaagc agctgggcac cgtgaccaca accgagcacg
     2521 agaacgccct gctgcggagc ttcgacaagt ttacaaccta cttctccggc ttttatgaga
     2581 acaggaagaa cgtgttcagc gccgaggata tcagcacagc catcccacac cgcatcgtgc
     2641 aggacaactt ccccaagttt aaggagaatt gtcacatctt cacacgcctg atcaccgccg
     2701 tgcccagcct gcgggagcac tttgagaacg tgaagaaggc catcggcatc ttcgtgagca
     2761 cctccatcga ggaggtgttt tccttccctt tttataacca gctgctgaca cagacccaga
     2821 tcgacctgta taaccagctg ctgggaggaa tctctcggga ggcaggcacc gagaagatca
     2881 agggcctgaa cgaggtgctg aatctggcca tccagaagaa tgatgagaca gcccacatca
     2941 tcgcctccct gccacacaga ttcatccccc tgtttaagca gatcctgtcc gataggaaca
     3001 ccctgtcttt catcctggag gagtttaaga gcgacgagga agtgatccag tccttctgca
     3061 agtacaagac actgctgaga aacgagaacg tgctggagac agccgaggcc ctgtttaacg
     3121 agctgaacag catcgacctg acacacatct tcatcagcca caagaagctg gagacaatca
     3181 gcagcgccct gtgcgaccac tgggatacac tgaggaatgc cctgtatgag cggagaatct
     3241 ccgagctgac aggcaagatc accaagtctg ccaaggagaa ggtgcagcgc agcctgaagc
     3301 acgaggatat caacctgcag gagatcatct ctgccgcagg caaggagctg agcgaggcct
     3361 tcaagcagaa aaccagcgag atcctgtccc acgcacacgc cgccctggat cagccactgc
     3421 ctacaaccct gaagaagcag gaggagaagg agatcctgaa gtctcagctg gacagcctgc
     3481 tgggcctgta ccacctgctg gactggtttg ccgtggatga gtccaacgag gtggaccccg
     3541 agttctctgc ccggctgacc ggcatcaagc tggagatgga gccttctctg agcttctaca
     3601 acaaggccag aaattatgcc accaagaagc cctactccgt ggagaagttc aagctgaact
     3661 ttcagatgcc tacactggcc tctggctggg acgtgaataa ggagaagaac aatggcgcca
     3721 tcctgtttgt gaagaacggc ctgtactatc tgggcatcat gccaaagcag aagggcaggt
     3781 ataaggccct gagcttcgag cccacagaga aaaccagcga gggctttgat aagatgtact
     3841 atgactactt ccctgatgcc gccaagatga tcccaaagtg cagcacccag ctgaaggccg
     3901 tgacagccca ctttcagacc cacacaaccc ccatcctgct gtccaacaat ttcatcgagc
     3961 ctctggagat cacaaaggag atctacgacc tgaacaatcc tgagaaggag ccaaagaagt
     4021 ttcagacagc ctacgccaag aaaaccggcg accagaaggg ctacagagag gccctgtgca
     4081 agtggatcga cttcacaagg gattttctgt ccaagtatac caagacaacc tctatcgatc
     4141 tgtctagcct gcggccatcc tctcagtata aggacctggg cgagtactat gccgagctga
     4201 atcccctgct gtaccacatc agcttccaga gaatcgccga gaaggagatc atggatgccg
     4261 tggagacagg caagctgtac ctgttccaga tctataacaa ggactttgcc aagggccacc
     4321 acggcaagcc taatctgcac acactgtatt ggaccggcct gttttctcca gagaacctgg
     4381 ccaagacaag catcaagctg aatggccagg ccgagctgtt ctaccgccct aagtccagga
     4441 tgaagaggat ggcacaccgg ctgggagaga agatgctgaa caagaagctg aaggatcaga
     4501 aaaccccaat ccccgacacc ctgtaccagg agctgtacga ctatgtgaat cacagactgt
     4561 cccacgacct gtctgatgag gccagggccc tgctgcccaa cgtgatcacc aaggaggtgt
     4621 ctcacgagat catcaaggat aggcgcttta ccagcgacaa gttctttttc cacgtgccta
     4681 tcacactgaa ctatcaggcc gccaattccc catctaagtt caaccagagg gtgaatgcct
     4741 acctgaagga gcaccccgag acacctatca tcggcatcgc ccggggcgag agaaacctga
     4801 tctatatcac agtgatcgac tccaccggca agatcctgga gcagcggagc ctgaacacca
     4861 tccagcagtt tgattaccag aagaagctgg acaacaggga gaaggagagg gtggcagcaa
     4921 ggcaggcctg gtctgtggtg ggcacaatca aggatctgaa gcagggctat ctgagccagg
     4981 tcatccacga gatcgtggac ctgatgatcc actaccaggc cgtggtggtg ctggagaacc
     5041 tgaatttcgg ctttaagagc aagaggaccg gcatcgccga gaaggccgtg taccagcagt
     5101 tcgagaagat gctgatcgat aagctgaatt gcctggtgct gaaggactat ccagcagaga
     5161 aagtgggagg cgtgctgaac ccataccagc tgacagacca gttcacctcc tttgccaaga
     5221 tgggcaccca gtctggcttc ctgttttacg tgcctgcccc atatacatct aagatcgatc
     5281 ccctgaccgg cttcgtggac cccttcgtgt ggaaaaccat caagaatcac gagagccgca
     5341 agcacttcct ggagggcttc gactttctgc actacgacgt gaaaaccggc gacttcatcc
     5401 tgcactttaa gatgaacaga aatctgtcct tccagagggg cctgcccggc tttatgcctg
     5461 catgggatat cgtgttcgag aagaacgaga cacagtttga cgccaagggc acccctttca
     5521 tcgccggcaa gagaatcgtg ccagtgatcg agaatcacag attcaccggc agataccggg
     5581 acctgtatcc tgccaacgag ctgatcgccc tgctggagga gaagggcatc gtgttcaggg
     5641 atggctccaa catcctgcca aagctgctgg agaatgacga ttctcacgcc atcgacacga
     5701 tggtggccct gatccgcagc gtgctgcaga tgcggaactc caatgccgcc acaggcgagg
     5761 actatatcaa cagccccgtg cgcgatctga atggcgtgtg cttcgactcc cggtttcaga
     5821 acccagagtg gccaatggac gccgatgcca atggcgccta ccacatcgcc ctgaagggcc
     5881 agctgctgct gaatcacctg aaggagagca aggatctgaa gctgcagaac ggcatctcca
     5941 atcaggactg gctggcctac atccaggagc tgcgcaacaa aaggccggcg gccacgaaaa
     6001 aggccggcca ggcaaaaaag aaaaagggat cctctggtgg ttctggagga tctggtggtt
     6061 ctactaatct gtcagatatt attgaaaagg agaccggtaa gcaactggtt atccaggaat
     6121 ccatcctcat gctcccagag gaggtggaag aagtcattgg gaacaagccg gaaagcgata
     6181 tactcgtgca caccgcctac gacgagagca ccgacgagaa tgtcatgctt ctgactagcg
     6241 acgcccctga atacaagcct tgggctctgg tcatacagga tagcaacggt gagaacaaga
     6301 ttaagatgct ctctggtggt tctcccaaga agaagaggaa agtctaaagc ggccgcactc
     6361 ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca
     6421 aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg
     6481 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     6541 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg
     6601 agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt
     6661 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     6721 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
     6781 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
     6841 tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc
     6901 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6961 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     7021 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7081 cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     7141 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     7201 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     7261 ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa
     7321 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7381 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc
     7441 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7501 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7561 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7621 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7681 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7741 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7801 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7861 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7921 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7981 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8041 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8101 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8161 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8221 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8281 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8341 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8401 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8461 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8521 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8581 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8641 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8701 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8761 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8821 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8881 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8941 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9001 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9061 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9121 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9181 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9241 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9301 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9361 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtcgaca
     9421 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     9481 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     9541 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     9601 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     9661 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     9721 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     9781 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
     9841 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
     9901 ggcggggggg gggg
//
