LOCUS       sequence-231034-        9685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoter expression plasmid for human codon optimized LbBE1.4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9685)
  AUTHORS   Kleinstiver BP, Sousa AA, Walton RT, Tak YE, Hsu JY, Clement K, 
            Welch MM, Horng JE, Malagon-Lopez J, Scarfo I, Maus MV, Pinello L, 
            Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas12a variants with increased activities and 
            improved targeting ranges for gene, epigenetic and base editing.
  JOURNAL   Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0.
  PUBMED    30742127
REFERENCE   2  (bases 1 to 9685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0."
COMMENT     Sequence Label: 
            pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV
            40)(LbBE1.4) (RTW1293)
FEATURES             Location/Qualifiers
     source          1..9685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          163..1179
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1103..1125
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1187..1206
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1272..1281
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1284..1304
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1314..1334
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1344..2027
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             2124..5804
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     CDS             5835..6083
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6096..6116
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6206..6225)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6271..6326
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6325..6344)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6687..6703)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6687..6703)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6700..6722)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6711..6727
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6735..6741,6742..6759,6760..6765))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6780..6801
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6860..7055
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6906..7041
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7061..7195
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7098..7117)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7152..7171
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7263..7280)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7434..8022)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7514..7533)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8193..8984,8985..9053))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8816..8835
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9054..9158)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        9189..9568
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggggggcgc gccmgggggg gggggggggg gggggggggg gggggggggg rggggggggg
       61 gggggggggg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
      121 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc
      181 cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc
      241 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc
      301 ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg
      361 gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg
      421 agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg
      481 ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc
      541 ggggggggct gcgrggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca
      601 gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct
      661 gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg
      721 ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc kcgggccggg
      781 gagggctcgg gggrggggcg cggcggcccc cggrgcgccg gcggctgtcg aggcgcggcg
      841 agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc
      901 caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg
      961 gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg
     1021 cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt
     1081 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc
     1141 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
     1201 attgtgctgt ctcatcattt tggcaaagaa ttctgcagtc gacggtaccg cgggcccggg
     1261 atccaccggt cgccaccatg ggcccaaaga aaaagaggaa agtcggcagt ggacctaaaa
     1321 agaaacgaaa ggttgggtca ggtagctcag agactggccc agtggctgtg gaccccacat
     1381 tgagacggcg gatcgagccc catgagtttg aggtattctt cgatccgaga gagctccgca
     1441 aggagacctg cctgctttac gaaattaatt gggggggccg gcactccatt tggcgacata
     1501 catcacagaa cactaacaag cacgtcgaag tcaacttcat cgagaagttc acgacagaaa
     1561 gatatttctg tccgaacaca aggtgcagca ttacctggtt tctcagctgg agcccatgcg
     1621 gcgaatgtag tagggccatc actgaattcc tgtcaaggta tccccacgtc actctgttta
     1681 tttacatcgc aaggctgtac caccacgctg acccccgcaa tcgacaaggc ctgcgggatt
     1741 tgatctcttc aggtgtgact atccaaatta tgactgagca ggagtcagga tactgctgga
     1801 gaaactttgt gaattatagc ccgagtaatg aagcccactg gcctaggtat ccccatctgt
     1861 gggtacgact gtacgttctt gaactgtact gcatcatact gggcctgcct ccttgtctca
     1921 acattctgag aaggaagcag ccacagctga cattctttac catcgctctt cagtcttgtc
     1981 attaccagcg actgccccca cacattctct gggccaccgg gttgaaatct ggtggttctt
     2041 ctggtggttc tagcggcagc gagactcccg ggacctcaga gtccgccaca cccgaaagtt
     2101 ccggagggag tagcggcggg tctagcaagc tggagaagtt tacaaactgc tactccctgt
     2161 ctaagaccct gaggttcaag gccatccctg tgggcaagac ccaggagaac atcgacaata
     2221 agcggctgct ggtggaggac gagaagagag ccgaggatta taagggcgtg aagaagctgc
     2281 tggatcgcta ctatctgtct tttatcaacg acgtgctgca cagcatcaag ctgaagaatc
     2341 tgaacaatta catcagcctg ttccggaaga aaaccagaac cgagaaggag aataaggagc
     2401 tggagaacct ggagatcaat ctgcggaagg agatcgccaa ggccttcaag ggcaacgagg
     2461 gctacaagtc cctgtttaag aaggatatca tcgagacaat cctgccagag ttcctggacg
     2521 ataaggacga gatcgccctg gtgaacagct tcaatggctt taccacagcc ttcaccggct
     2581 tctttgataa cagagagaat atgttttccg aggaggccaa gagcacatcc atcgccttca
     2641 ggtgtatcaa cgagaatctg acccgctaca tctctaatat ggacatcttc gagaaggtgg
     2701 acgccatctt tgataagcac gaggtgcagg agatcaagga gaagatcctg aacagcgact
     2761 atgatgtgga ggatttcttt gagggcgagt tctttaactt tgtgctgaca caggagggca
     2821 tcgacgtgta taacgccatc atcggcggct tcgtgaccga gagcggcgag aagatcaagg
     2881 gcctgaacga gtacatcaac ctgtataatc agaaaaccaa gcagaagctg cctaagttta
     2941 agccactgta taagcaggtg ctgagcgatc gggagtctct gagcttctac ggcgagggct
     3001 atacatccga tgaggaggtg ctggaggtgt ttagaaacac cctgaacaag aacagcgaga
     3061 tcttcagctc catcaagaag ctggagaagc tgttcaagaa ttttgacgag tactctagcg
     3121 ccggcatctt tgtgaagaac ggccccgcca tcagcacaat ctccaaggat atcttcggcg
     3181 agtggaacgt gatccgggac aagtggaatg ccgagtatga cgatatccac ctgaagaaga
     3241 aggccgtggt gaccgagaag tacgaggacg atcggagaaa gtccttcaag aagatcggct
     3301 ccttttctct ggagcagctg caggagtacg ccgacgccga tctgtctgtg gtggagaagc
     3361 tgaaggagat catcatccag aaggtggatg agatctacaa ggtgtatggc tcctctgaga
     3421 agctgttcga cgccgatttt gtgctggaga agagcctgaa gaagaacgac gccgtggtgg
     3481 ccatcatgaa ggacctgctg gattctgtga agagcttcga gaattacatc aaggccttct
     3541 ttggcgaggg caaggagaca aacagggacg agtccttcta tggcgatttt gtgctggcct
     3601 acgacatcct gctgaaggtg gaccacatct acgatgccat ccgcaattat gtgacccaga
     3661 agccctactc taaggataag ttcaagctgt attttcagaa ccctcagttc atgggcggct
     3721 gggacaagga taaggagaca gactatcggg ccaccatcct gagatacggc tccaagtact
     3781 atctggccat catggataag aagtacgcca agtgcctgca gaagatcgac aaggacgatg
     3841 tgaacggcaa ttacgagaag atcaactata agctgctgcc cggccctaat aagatgctgc
     3901 caaaggtgtt cttttctaag aagtggatgg cctactataa ccccagcgag gacatccaga
     3961 agatctacaa gaatggcaca ttcaagaagg gcgatatgtt taacctgaat gactgtcaca
     4021 agctgatcga cttctttaag gatagcatct cccggtatcc aaagtggtcc aatgcctacg
     4081 atttcaactt ttctgagaca gagaagtata aggacatcgc cggcttttac agagaggtgg
     4141 aggagcaggg ctataaggtg agcttcgagt ctgccagcaa gaaggaggtg gataagctgg
     4201 tggaggaggg caagctgtat atgttccaga tctataacaa ggacttttcc gataagtctc
     4261 acggcacacc caatctgcac accatgtact tcaagctgct gtttgacgag aacaatcacg
     4321 gacagatcag gctgagcgga ggagcagagc tgttcatgag gcgcgcctcc ctgaagaagg
     4381 aggagctggt ggtgcaccca gccaactccc ctatcgccaa caagaatcca gataatccca
     4441 agaaaaccac aaccctgtcc tacgacgtgt ataaggataa gaggttttct gaggaccagt
     4501 acgagctgca catcccaatc gccatcaata agtgccccaa gaacatcttc aagatcaata
     4561 cagaggtgcg cgtgctgctg aagcacgacg ataaccccta tgtgatcggc atcgccaggg
     4621 gcgagcgcaa tctgctgtat atcgtggtgg tggacggcaa gggcaacatc gtggagcagt
     4681 attccctgaa cgagatcatc aacaacttca acggcatcag gatcaagaca gattaccact
     4741 ctctgctgga caagaaggag aaggagaggt tcgaggcccg ccagaactgg acctccatcg
     4801 agaatatcaa ggagctgaag gccggctata tctctcaggt ggtgcacaag atctgcgagc
     4861 tggtggagaa gtacgatgcc gtgatcgccc tggaggacct gaactctggc tttaagaata
     4921 gccgcgtgaa ggtggagaag caggtgtatc agaagttcga gaagatgctg atcgataagc
     4981 tgaactacat ggtggacaag aagtctaatc cttgtgcaac aggcggcgcc ctgaagggct
     5041 atcagatcac caataagttc gagagcttta agtccatgtc tacccagaac ggcttcatct
     5101 tttacatccc tgcctggctg acatccaaga tcgatccatc taccggcttt gtgaacctgc
     5161 tgaaaaccaa gtataccagc atcgccgatt ccaagaagtt catcagctcc tttgacagga
     5221 tcatgtacgt gcccgaggag gatctgttcg agtttgccct ggactataag aacttctctc
     5281 gcacagacgc cgattacatc aagaagtgga agctgtactc ctacggcaac cggatcagaa
     5341 tcttccggaa tcctaagaag aacaacgtgt tcgactggga ggaggtgtgc ctgaccagcg
     5401 cctataagga gctgttcaac aagtacggca tcaattatca gcagggcgat atcagagccc
     5461 tgctgtgcga gcagtccgac aaggccttct actctagctt tatggccctg atgagcctga
     5521 tgctgcagat gcggaacagc atcacaggcc gcaccgacgt ggattttctg atcagccctg
     5581 tgaagaactc cgacggcatc ttctacgata gccggaacta tgaggcccag gagaatgcca
     5641 tcctgccaaa gaacgccgac gccaatggcg cctataacat cgccagaaag gtgctgtggg
     5701 ccatcggcca gttcaagaag gccgaggacg agaagctgga taaggtgaag atcgccatct
     5761 ctaacaagga gtggctggag tacgcccaga ccagcgtgaa gcactctggt ggttctggag
     5821 gatctggtgg ttctactaat ctgtcagata ttattgaaaa ggagaccggt aagcaactgg
     5881 ttatccagga atccatcctc atgctcccag aggaggtgga agaagtcatt gggaacaagc
     5941 cggaaagcga tatactcgtg cacaccgcct acgacgagag caccgacgag aatgtcatgc
     6001 ttctgactag cgacgcccct gaatacaagc cttgggctct ggtcatacag gatagcaacg
     6061 gtgagaacaa gattaagatg ctctctggtg gttctcccaa gaagaagagg aaagtctaaa
     6121 gcggccgcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg
     6181 ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca
     6241 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag
     6301 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa
     6361 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca
     6421 tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
     6481 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
     6541 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
     6601 tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc
     6661 tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6721 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6781 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6841 ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc
     6901 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6961 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     7021 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta
     7081 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7141 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg
     7201 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     7261 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7321 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7381 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7441 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7501 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7561 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7621 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7681 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7741 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7801 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7861 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7921 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7981 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8041 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8101 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8161 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8221 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8281 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8341 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8401 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8461 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8521 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8581 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8641 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8701 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8761 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8821 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8881 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8941 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9001 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9061 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9121 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9181 cctgggtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     9241 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     9301 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     9361 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     9421 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     9481 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     9541 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
     9601 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
     9661 tgcagcgatg ggggcggggg ggggg
//
