LOCUS       sequence-231038-        9915 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoter expression plasmid for human codon optimized enAsBE1.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9915)
  AUTHORS   Kleinstiver BP, Sousa AA, Walton RT, Tak YE, Hsu JY, Clement K, 
            Welch MM, Horng JE, Malagon-Lopez J, Scarfo I, Maus MV, Pinello L, 
            Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas12a variants with increased activities and 
            improved targeting ranges for gene, epigenetic and base editing.
  JOURNAL   Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0.
  PUBMED    30742127
REFERENCE   2  (bases 1 to 9915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0."
COMMENT     Sequence Label: 
            pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(n
            ucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1) (RTW1352)
FEATURES             Location/Qualifiers
     source          1..9915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          663..1679
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1603..1625
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1687..1706
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1772..1781
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1778..2467
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MGSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINW
                     GGRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEF
                     LSRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPS
                     NEAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPH
                     ILWATGLK"
     CDS             2564..6481
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     CDS             6482..6529
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6566..6814
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6827..6847
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6937..6956)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7002..7057
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7056..7075)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7418..7434)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7418..7434)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7431..7453)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7442..7458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7466..7472,7473..7490,7491..7496))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7511..7532
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7591..7786
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7637..7772
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7792..7926
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7829..7848)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7883..7902
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7994..8011)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8165..8753)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8245..8264)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8924..9715,9716..9784))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9547..9566
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9785..9889)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggncgcg cgccrggcgg ggsggggsgg ggsgrggggs
      541 ggggsggggs grggsggrgr ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttctgcagtc
     1741 gacggtaccg cgggcccggg atccaccggt cgccaccatg ggcagctcag agactggccc
     1801 agtggctgtg gaccccacat tgagacggcg gatcgagccc catgagtttg aggtattctt
     1861 cgatccgaga gagctccgca aggagacctg cctgctttac gaaattaatt gggggggccg
     1921 gcactccatt tggcgacata catcacagaa cactaacaag cacgtcgaag tcaacttcat
     1981 cgagaagttc acgacagaaa gatatttctg tccgaacaca aggtgcagca ttacctggtt
     2041 tctcagctgg agcccatgcg gcgaatgtag tagggccatc actgaattcc tgtcaaggta
     2101 tccccacgtc actctgttta tttacatcgc aaggctgtac caccacgctg acccccgcaa
     2161 tcgacaaggc ctgcgggatt tgatctcttc aggtgtgact atccaaatta tgactgagca
     2221 ggagtcagga tactgctgga gaaactttgt gaattatagc ccgagtaatg aagcccactg
     2281 gcctaggtat ccccatctgt gggtacgact gtacgttctt gaactgtact gcatcatact
     2341 gggcctgcct ccttgtctca acattctgag aaggaagcag ccacagctga cattctttac
     2401 catcgctctt cagtcttgtc attaccagcg actgccccca cacattctct gggccaccgg
     2461 gttgaaatct ggtggttctt ctggtggttc tagcggcagc gagactcccg ggacctcaga
     2521 gtccgccaca cccgaaagtt ccggagggag tagcggcggg tctacacagt tcgagggctt
     2581 taccaacctg tatcaggtga gcaagacact gcggtttgag ctgatcccac agggcaagac
     2641 cctgaagcac atccaggagc agggcttcat cgaggaggac aaggcccgca atgatcacta
     2701 caaggagctg aagcccatca tcgatcggat ctacaagacc tatgccgacc agtgcctgca
     2761 gctggtgcag ctggattggg agaacctgag cgccgccatc gactcctata gaaaggagaa
     2821 aaccgaggag acaaggaacg ccctgatcga ggagcaggcc acatatcgca atgccatcca
     2881 cgactacttc atcggccgga cagacaacct gaccgatgcc atcaataaga gacacgccga
     2941 gatctacaag ggcctgttca aggccgagct gtttaatggc aaggtgctga agcagctggg
     3001 caccgtgacc acaaccgagc acgagaacgc cctgctgcgg agcttcgaca agtttacaac
     3061 ctacttctcc ggcttttata gaaacaggaa gaacgtgttc agcgccgagg atatcagcac
     3121 agccatccca caccgcatcg tgcaggacaa cttccccaag tttaaggaga attgtcacat
     3181 cttcacacgc ctgatcaccg ccgtgcccag cctgcgggag cactttgaga acgtgaagaa
     3241 ggccatcggc atcttcgtga gcacctccat cgaggaggtg ttttccttcc ctttttataa
     3301 ccagctgctg acacagaccc agatcgacct gtataaccag ctgctgggag gaatctctcg
     3361 ggaggcaggc accgagaaga tcaagggcct gaacgaggtg ctgaatctgg ccatccagaa
     3421 gaatgatgag acagcccaca tcatcgcctc cctgccacac agattcatcc ccctgtttaa
     3481 gcagatcctg tccgatagga acaccctgtc tttcatcctg gaggagttta agagcgacga
     3541 ggaagtgatc cagtccttct gcaagtacaa gacactgctg agaaacgaga acgtgctgga
     3601 gacagccgag gccctgttta acgagctgaa cagcatcgac ctgacacaca tcttcatcag
     3661 ccacaagaag ctggagacaa tcagcagcgc cctgtgcgac cactgggata cactgaggaa
     3721 tgccctgtat gagcggagaa tctccgagct gacaggcaag atcaccaagt ctgccaagga
     3781 gaaggtgcag cgcagcctga agcacgagga tatcaacctg caggagatca tctctgccgc
     3841 aggcaaggag ctgagcgagg ccttcaagca gaaaaccagc gagatcctgt cccacgcaca
     3901 cgccgccctg gatcagccac tgcctacaac cctgaagaag caggaggaga aggagatcct
     3961 gaagtctcag ctggacagcc tgctgggcct gtaccacctg ctggactggt ttgccgtgga
     4021 tgagtccaac gaggtggacc ccgagttctc tgcccggctg accggcatca agctggagat
     4081 ggagccttct ctgagcttct acaacaaggc cagaaattat gccaccaaga agccctactc
     4141 cgtggagaag ttcaagctga actttcagat gcctacactg gccagaggct gggacgtgaa
     4201 tagagagaag aacaatggcg ccatcctgtt tgtgaagaac ggcctgtact atctgggcat
     4261 catgccaaag cagaagggca ggtataaggc cctgagcttc gagcccacag agaaaaccag
     4321 cgagggcttt gataagatgt actatgacta cttccctgat gccgccaaga tgatcccaaa
     4381 gtgcagcacc cagctgaagg ccgtgacagc ccactttcag acccacacaa cccccatcct
     4441 gctgtccaac aatttcatcg agcctctgga gatcacaaag gagatctacg acctgaacaa
     4501 tcctgagaag gagccaaaga agtttcagac agcctacgcc aagaaaaccg gcgaccagaa
     4561 gggctacaga gaggccctgt gcaagtggat cgacttcaca agggattttc tgtccaagta
     4621 taccaagaca acctctatcg atctgtctag cctgcggcca tcctctcagt ataaggacct
     4681 gggcgagtac tatgccgagc tgaatcccct gctgtaccac atcagcttcc agagaatcgc
     4741 cgagaaggag atcatggatg ccgtggagac aggcaagctg tacctgttcc agatctataa
     4801 caaggacttt gccaagggcc accacggcaa gcctaatctg cacacactgt attggaccgg
     4861 cctgttttct ccagagaacc tggccaagac aagcatcaag ctgaatggcc aggccgagct
     4921 gttctaccgc cctaagtcca ggatgaagag gatggcacac cggctgggag agaagatgct
     4981 gaacaagaag ctgaaggatc agaaaacccc aatccccgac accctgtacc aggagctgta
     5041 cgactatgtg aatcacagac tgtcccacga cctgtctgat gaggccaggg ccctgctgcc
     5101 caacgtgatc accaaggagg tgtctcacga gatcatcaag gataggcgct ttaccagcga
     5161 caagttcttt ttccacgtgc ctatcacact gaactatcag gccgccaatt ccccatctaa
     5221 gttcaaccag agggtgaatg cctacctgaa ggagcacccc gagacaccta tcatcggcat
     5281 cgcccggggc gagagaaacc tgatctatat cacagtgatc gactccaccg gcaagatcct
     5341 ggagcagcgg agcctgaaca ccatccagca gtttgattac cagaagaagc tggacaacag
     5401 ggagaaggag agggtggcag caaggcaggc ctggtctgtg gtgggcacaa tcaaggatct
     5461 gaagcagggc tatctgagcc aggtcatcca cgagatcgtg gacctgatga tccactacca
     5521 ggccgtggtg gtgctggaga acctgaattt cggctttaag agcaagagga ccggcatcgc
     5581 cgagaaggcc gtgtaccagc agttcgagaa gatgctgatc gataagctga attgcctggt
     5641 gctgaaggac tatccagcag agaaagtggg aggcgtgctg aacccatacc agctgacaga
     5701 ccagttcacc tcctttgcca agatgggcac ccagtctggc ttcctgtttt acgtgcctgc
     5761 cccatataca tctaagatcg atcccctgac cggcttcgtg gaccccttcg tgtggaaaac
     5821 catcaagaat cacgagagcc gcaagcactt cctggagggc ttcgactttc tgcactacga
     5881 cgtgaaaacc ggcgacttca tcctgcactt taagatgaac agaaatctgt ccttccagag
     5941 gggcctgccc ggctttatgc ctgcatggga tatcgtgttc gagaagaacg agacacagtt
     6001 tgacgccaag ggcacccctt tcatcgccgg caagagaatc gtgccagtga tcgagaatca
     6061 cagattcacc ggcagatacc gggacctgta tcctgccaac gagctgatcg ccctgctgga
     6121 ggagaagggc atcgtgttca gggatggctc caacatcctg ccaaagctgc tggagaatga
     6181 cgattctcac gccatcgaca cgatggtggc cctgatccgc agcgtgctgc agatgcggaa
     6241 ctccaatgcc gccacaggcg aggactatat caacagcccc gtgcgcgatc tgaatggcgt
     6301 gtgcttcgac tcccggtttc agaacccaga gtggccaatg gacgccgatg ccaatggcgc
     6361 ctaccacatc gccctgaagg gccagctgct gctgaatcac ctgaaggaga gcaaggatct
     6421 gaagctgcag aacggcatct ccaatcagga ctggctggcc tacatccagg agctgcgcaa
     6481 caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg gatcctctgg
     6541 tggttctgga ggatctggtg gttctactaa tctgtcagat attattgaaa aggagaccgg
     6601 taagcaactg gttatccagg aatccatcct catgctccca gaggaggtgg aagaagtcat
     6661 tgggaacaag ccggaaagcg atatactcgt gcacaccgcc tacgacgaga gcaccgacga
     6721 gaatgtcatg cttctgacta gcgacgcccc tgaatacaag ccttgggctc tggtcataca
     6781 ggatagcaac ggtgagaaca agattaagat gctctctggt ggttctccca agaagaagag
     6841 gaaagtctaa agcggccgca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     6901 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
     6961 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     7021 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     7081 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
     7141 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
     7201 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     7261 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     7321 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag
     7381 atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7441 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7501 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7561 agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc
     7621 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7681 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     7741 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt
     7801 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7861 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7921 tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7981 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     8041 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     8101 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8161 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8221 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8281 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8341 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8401 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8461 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8521 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8581 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     8641 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8701 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8761 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8821 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8881 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8941 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9001 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9061 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9121 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9181 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9241 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9301 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9361 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9421 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9481 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9541 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9601 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9661 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9721 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9781 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9841 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9901 gaaaagtgcc acctg
//
