LOCUS       sequence-231068-        2819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for lambda cI (repressor of pL or
            pOR1-OR2; use C64m for temperature-sensitive version). Please see 
            Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2819)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C24m
FEATURES             Location/Qualifiers
     source          1..2819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2..715
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     misc_feature    727..747
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(753..770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(924..1512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1004..1023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1683..2474,2475..2543))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2306..2325
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2544..2648)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2716..2734
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2738..2781)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2784..2805
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa
       61 agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa
      121 gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc
      181 ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc
      241 aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag
      301 tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag
      361 aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc
      421 tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag
      481 ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt
      541 ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg
      601 tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg
      661 ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gctgaaggta
      721 gagacctact agtagcggcc gctgcaggct tcctcgctca ctgactcgct gcgctcggtc
      781 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      841 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      901 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
      961 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1021 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1081 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1141 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1201 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1261 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1321 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1381 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1441 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1501 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1561 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1621 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1681 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1741 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1801 cccagtgctg caatgatacc gcgcgaccca cgctcaccgg ctccagattt atcagcaata
     1861 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1921 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1981 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2041 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2101 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2161 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     2221 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     2281 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     2341 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     2401 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     2461 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     2521 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     2581 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2641 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     2701 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
     2761 gctttcgcta aggatgattt ctggaattcg cggccgcttc tagagactag tgggtctca
//
