LOCUS       sequence-231069-        2729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for TetR (tet repressor; 
            identical to C2d but without ssrA degradation tag). Please see 
            Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2729)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C27m
FEATURES             Location/Qualifiers
     source          1..2729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     88..106
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(110..153)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    156..177
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             193..816
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(266..285)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    828..848
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(854..871)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1025..1613)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1105..1124)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1784..2575,2576..2644))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2407..2426
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(2645..2729,1..20))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
       61 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa
      121 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagagact
      181 agtgggtctc aaatgtccag attagataaa agtaaagtga ttaacagcgc attagagctg
      241 cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta
      301 gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc
      361 attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa
      421 gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga
      481 gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa
      541 ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct
      601 gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa
      661 gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta
      721 tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga
      781 ttagaaaaac aacttaaatg tgaaagtggg tcctaaaggt agagacctac tagtagcggc
      841 cgctgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
      901 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
      961 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1021 cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1081 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1141 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1201 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1261 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1321 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1381 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1441 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1501 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1561 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1621 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1681 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1741 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     1801 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1861 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     1921 cgcgcgaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     1981 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2041 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     2101 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2161 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2221 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     2281 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     2341 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     2401 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     2461 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     2521 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     2581 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     2641 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     2701 gatacatatt tgaatgtatt tagaaaaat
//
