LOCUS       sequence-231070-        3606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for Bxb1 integrase. See DOI: 
            10.1007/s00438-006-0129-5. Please see Supplemental Documents for 
            annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3606)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C31m
FEATURES             Location/Qualifiers
     source          1..3606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     395..414
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(633..737)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     805..823
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(827..870)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    873..894
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             907..2412
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MVRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDL
                     DVSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKL
                     VVSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWG
                     YLPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQ
                     GREPQGREWSATALKRSMISGAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRA
                     ELVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGT
                     VAMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRA
                     GSPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRS
                     MNVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     primer_bind     complement(2448..2465)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2619..3207)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2699..2718)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3378..3606,1..563,564..632))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
       61 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
      121 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
      181 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
      241 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
      301 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
      361 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
      421 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
      481 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
      541 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
      601 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
      661 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
      721 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
      781 attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa
      841 aaatccttag ctttcgctaa ggatgatttc tggaattcgc ggccgcttct agagactagg
      901 tctcaaatgg tgagagccct ggtagtcatc cgcctgtccc gcgtcaccga tgctacgact
      961 tcaccggagc gtcagctgga gtcttgccag cagctctgcg cccagcgcgg ctgggacgtc
     1021 gtcggggtag cggaggatct ggacgtctcc ggggcggtcg atccgttcga ccggaagcgc
     1081 agaccgaacc tggcccggtg gctagcgttc gaggagcaac cgtttgacgt gatcgtggcg
     1141 taccgggtag atcggttgac ccgatcgatc cggcatcttc agcagctggt ccactgggcc
     1201 gaggaccaca agaagctggt cgtctccgcg accgaagcgc acttcgatac gacgacgccg
     1261 tttgcggcgg tcgtcatcgc gcttatggga acggtggcgc agatggaatt agaagcgatc
     1321 aaagagcgga accgttcggc tgcgcatttc aatatccgcg ccgggaaata ccgaggatcc
     1381 ctgccgccgt ggggatacct gcctacgcgc gtggacgggg agtggcggct ggtgccggac
     1441 cctgtgcagc gagagcgcat cctcgaggtg tatcaccgcg tcgtcgacaa ccacgagccg
     1501 ctgcatctgg tggcccacga cctgaaccgg cgtggtgtcc tgtcgccgaa ggactacttc
     1561 gcgcagctgc aaggccgcga gccgcagggc cgggagtggt cggctaccgc gctgaagcga
     1621 tcgatgatct ccggggcgat gctcgggtac gcgactctga acggtaagac cgtccgagac
     1681 gacgacggag ccccgctggt gcgggctgag ccgatcctga cccgtgagca gctggaggcg
     1741 ctgcgcgccg agctcgtcaa gacctcccgg gcgaagcccg cggtgtctac cccgtcgctg
     1801 ctgctgcggg tgttgttctg cgcggtgtgc ggggagcccg cgtacaagtt cgccggggga
     1861 ggacgtaagc acccgcgcta ccgctgccgc tcgatggggt tcccgaagca ctgcgggaac
     1921 ggcacggtgg cgatggccga gtgggacgcg ttctgcgagg agcaggtact ggatctgctc
     1981 ggggacgcgg agcgtctgga gaaagtctgg gtagcgggct cggactccgc ggtcgaactc
     2041 gcggaggtga acgcggagct ggtggacctg acgtcgctga tcggctcccc ggcctaccgg
     2101 gcgggctctc cgcagcgaga agcactggat gcccgtattg cggcgctggc cgcgcggcaa
     2161 gaggagctgg agggcctgga ggctcgcccg tctggctggg agtggcgcga aaccgggcag
     2221 cggttcgggg actggtggcg ggagcaggac accgcggcaa agaacacctg gcttcggtcg
     2281 atgaacgttc ggctgacgtt cgacgtccgc ggcgggctga ctcgcacgat cgacttcggg
     2341 gatcttcagg agtacgagca gcatctcagg ctcggcagcg tggtcgaacg gctacacacc
     2401 gggatgtcgt aaaggttgag accctagtag cggccgctgc aggcttcctc gctcactgac
     2461 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2521 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2581 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct
     2641 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2701 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2761 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2821 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2881 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2941 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3001 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     3061 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3121 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3181 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3241 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3301 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3361 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     3421 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     3481 ggcttaccat ctggccccag tgctgcaatg ataccgcgcg acccacgctc accggctcca
     3541 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     3601 ttatcc
//
