LOCUS       sequence-231072-        2831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for CinR (Quorum-sensing receptor
            for 3OC14). Please see Supplemental Documents for annotated Genbank 
            file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2831)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C32m
FEATURES             Location/Qualifiers
     source          1..2831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    739..759
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(765..782)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(936..1524)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1016..1035)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1695..2486,2487..2555))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2318..2337
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2556..2660)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2728..2746
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2750..2793)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2796..2817
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatgattgag aatacctata gcgaaaagtt cgagtccgcg ttcgaacaga tcaaggcggc
       61 ggccaacgtg gatgccgcca tccgtattct ccaggcggaa tataacctcg atttcgtcac
      121 ctaccatctc gcccagacga tcgcgagcaa gatcgatccg cccttcgtgc gcaccaccta
      181 tccggatgcc tgggtttccc gctacctcct caacagctat gtgaaggtcg atccgatcgt
      241 caagcagggc ttcgaacgcc agctgccctt cgactggagc gaggtcgaac cgacgccgga
      301 ggcctatgcc atgctggtcg acgcccagaa acacggcatc ggtggcaatg gctactccat
      361 ccccgtcgcc gacaaggcgc agcgccgcgc cctgctgtcg ctgaatgccc gtataccggc
      421 cgacgaatgg accgagcttg tgcgccgctg ccgcaacgag tggatcgaga tcgcccatct
      481 gatccaccgc aaggccgtct atgagctgca tggcgaaaac gatccggtgc cggcattgtc
      541 gccgcgcgag atcgagtgtc tgcactggac cgccctcggc aaggattaca aggatatttc
      601 ggtcatcctg ggcatatcag agcataccac acgcgattac ctgaagaccg cccgcttcaa
      661 gctcggctgc gccacgatct cggccgccgc gtcgcgggct gttcaattgc gcatcatcaa
      721 tccctgaagg tagagaccta ctagtagcgg ccgctgcagg cttcctcgct cactgactcg
      781 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      841 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      901 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
      961 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1021 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1081 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1141 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1201 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1261 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1321 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     1381 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1441 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1501 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1561 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1621 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1681 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1741 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1801 ttaccatctg gccccagtgc tgcaatgata ccgcgcgacc cacgctcacc ggctccagat
     1861 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1921 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1981 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     2041 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     2101 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     2161 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     2221 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     2281 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     2341 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     2401 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     2461 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     2521 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     2581 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2641 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2701 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa
     2761 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagagact
     2821 agtgggtctc a
//
